[논문]Evaluation of Protein Expression in Housekeeping Genes across Multiple Tissues in Rats

Kim, Hye Jeong; Na, Jong In; Min, Byung Woo; Na, Joo Young; Lee, Kyung Hwa; Lee, Jae Hyuk; Lee, Young Jik; Kim, Hyung Seok; Park, Jong Tae

doi:10.4132/KoreanJPathol.2014.48.3.193

Evaluation of Protein Expression in Housekeeping Genes across Multiple Tissues in Rats 원문보기

The Korean journal of pathology, v.48 no.3, 2014년, pp.193 - 200

Kim, Hye Jeong (Department of Forensic Medicine, Chonnam National University Medical School, Gwangju, Korea.) , Na, Jong In (Department of Forensic Medicine, Chonnam National University Medical School, Gwangju, Korea.) , Min, Byung Woo (Department of Forensic Medicine, Chonnam National University Medical School, Gwangju, Korea.) , Na, Joo Young (Department of Forensic Medicine, Chonnam National University Medical School, Gwangju, Korea.) , Lee, Kyung Hwa (Department of Pathology, Chonnam National University Medical School, Gwangju, Korea.) , Lee, Jae Hyuk (Department of Pathology, Chonnam National University Medical School, Gwangju, Korea.) , Lee, Young Jik (Department of Forensic Medicine, Chonnam National University Medical School, Gwangju, Korea.) , Kim, Hyung Seok (Department of Forensic Medicine, Chonnam National University Medical School, Gwangju, Korea.) , Park, Jong Tae (Department of Forensic Medicine, Chonnam National University Medical School, Gwangju, Korea.)

Abstract ▼ AI-Helper

BackgroundHousekeeping genes, which show constant protein expression patterns between different tissue types, are very important in molecular biological studies as an internal control for protein research.MethodsThe protein expression profiles of seven housekeeping genes (HPRT1, PPIA, GYS1, TBP, YWHAZ, GAPDH and ACTB) in various rat tissues (cerebrum, cerebellum, cardiac ventricle and atrium, psoas muscle, femoral muscle, liver, spleen, kidney, and aorta) were analyzed by Western blot and compared by coefficient of variation (CV).ResultsHPRT1 was stably expressed (CV≤10%) in six tissues (cerebrum, cerebellum, ventricle, femoral muscle, spleen, and kidney), PPIA was stably expressed in five tissues (cerebrum, cerebellum, ventricle, spleen and kidney), YWHAZ was stably expressed in three tissues (cerebrum, cerebellum, and kidney), and GAPDH was stably expressed in four tissues (cerebrum, ventricle, psoas muscle, and kidney). In comparison, GYS1, TBP, and ACTB were found to have CV values over 10% in all tissues. Of the seven genes examined, four (HPRT1, PPIA, YWHAZ, and GAPDH) were found to be stably expressed across multiple organs, with low CV values (≤10%).ConclusionsThese results will provide fundamental information regarding internal controls for protein expression studies and can be used for analysis of postmortem protein degradation patterns in forensic medicine.

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Evaluation of Protein Expression in Housekeeping Genes across Multiple Tissues in Rats 원문보기

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Evaluation of Protein Expression in Housekeeping Genes across Multiple Tissues in Rats 원문보기

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