[논문]Drug resistance of oral bacteria to new antibacterial dental monomer dimethylaminohexadecyl methacrylate

Wang, Suping; Wang, Haohao; Ren, Biao; Li, Xiaodong; Wang, Lin; Zhou, Han; Weir, Michael D.; Zhou, Xuedong; Masri, Radi M.; Oates, Thomas W.; Cheng, Lei; Xu, Hockin H. K.

doi:10.1038/s41598-018-23831-3

Drug resistance of oral bacteria to new antibacterial dental monomer dimethylaminohexadecyl methacrylate 원문보기

Scientific reports, v.8, 2018년, pp.5509 - 5509

Wang, Suping (State Key Laboratory of Oral Diseases & National Clinical Research Center for Oral Diseases & Deptartment of Cariology and Endodonics West China Hospital of Stomatology, Sichuan University, Chengdu, 610041, China) , Wang, Haohao (Department of Advanced Oral Sciences and Therapeutics, University of Maryland Dental School, Baltimore, MD, 21201, USA) , Ren, Biao (State Key Laboratory of Oral Diseases & National Clinical Research Center for Oral Diseases & Deptartment of Cariology and Endodonics West China Hospital of Stomatology, Sichuan University, Chengdu, 610041, China) , Li, Xiaodong (Department of Advanced Oral Sciences and Therapeutics, University of Maryland Dental School, Baltimore, MD, 21201, USA) , Wang, Lin (State Key Laboratory of Oral Diseases & National Clinical Research Center for Oral Diseases & Deptartment of Cariology and Endodonics West China Hospital of Stomatology, Sichuan University, Chengdu, 610041, China) , Zhou, Han (Department of Oral Medicine, School of Stomatology, Zhejiang University, Hangzhou, Zhejiang, China) , Weir, Michael D. (VIP Integrat) , Zhou, Xuedong , Masri, Radi M. , Oates, Thomas W. , Cheng, Lei , Xu, Hockin H. K.

Abstract ▼ AI-Helper

Only two reports exist on drug-resistance of quaternary ammonium monomers against oral bacteria; both studies tested planktonic bacteria for 10 passages, and neither study tested biofilms or resins. The objectives of this study were to investigate the drug-resistance of Streptococcus mutans, Streptococcus sanguinis and Streptococcus gordonii against dimethylaminohexadecyl methacrylate (DMAHDM), and to evaluate biofilms on resins with repeated exposures for 20 passages for the first time. DMAHDM, dimethylaminododecyl methacrylate (DMADDM) and chlorhexidine (CHX) were tested with planktonic bacteria. Biofilms were grown on a resin containing 3% DMAHDM. Minimum-inhibitory concentrations were measured. To detect drug-resistance, the survived bacteria from the previous passage were used as inoculum for the next passage for repeated exposures. S. gordonii developed drug-resistance against DMADDM and CHX, but not against DMAHDM. Biofilm colony-forming units (CFU) on DMAHDM-resin was reduced by 3–4 log; there was no difference from passages 1 to 20 (p > 0.1). No drug-resistance to DMAHDM was detected for all three bacterial species. In conclusion, this study showed that DMAHDM induced no drug-resistance, and DMAHDM-resin reduced biofilm CFU by 3–4 log, with no significant change from 1 to 20 passages. DMAHDM with potent antibacterial activities and no drug-resistance is promising for dental applications.

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표제어: PCR

동의어: Packet Collision Rate

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Drug resistance of oral bacteria to new antibacterial dental monomer dimethylaminohexadecyl methacrylate 원문보기

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