[논문]Recombinant agarase increases the production of reducing sugars from HCl-treated Gracilaria verrucosa, a red algae

Kim, Se Won; Kim, Yong-Woon; Hong, Chae-Hwan; Lyo, In-Woong; Lim, Ho-Dong; Kim, Geun-Joong; Shin, Hyun-Jae

doi:10.1016/j.algal.2017.01.008

[해외논문] Recombinant agarase increases the production of reducing sugars from HCl-treated Gracilaria verrucosa, a red algae

Algal research, v.31, 2018년, pp.517 - 524

Kim, Se Won (Department of Biochemical and Polymer Engineering, Chosun University) , Kim, Yong-Woon (Department of Biochemical and Polymer Engineering, Chosun University) , Hong, Chae-Hwan (Research and Development Division, Hyundai Motor Group) , Lyo, In-Woong (Research and Development Division, Hyundai Motor Group) , Lim, Ho-Dong (Department of Biological Sciences, Chonnam National University) , Kim, Geun-Joong (Department of Biological Sciences, Chonnam National University) , Shin, Hyun-Jae (Department of Biochemical and Polymer Engineering, Chosun University)

Abstract ▼ AI-Helper

Abstract One of the most intensively studied species in red algae is Gracilaria spp., which is also a highly abundantly produced species annually in Asian countries including Korea and Japan. Much of the recent work on production of reducing sugars from Gracilaria spp. has concentrated on a combined process of physicochemical and biochemical treatments using acids and commercial enzymes. To the best of our knowledge, this study is first demonstration of combined acid and enzymatic hydrolysis using recombinant agarase to produce total reducing sugar (TRS) from Gracilaria verrucosa. By performing acid hydrolysis under the specified condition (2% biomass, 0.1N HCl, 121°C, 15min), a 34.9% TRS yield was obtained. Subsequent enzymatic hydrolysis using two recombinant agarases (aga 50D 0.5U/mg+NABH 0.5U/mg), resulted in a 47.4% TRS yield was achieved. The TRS yield obtained from acid and enzymatic hydrolysates was 5% higher than that of previous study with commercial enzyme. These results served to demonstrate that feasibility of enzymatic hydrolysis using the recombinant agarases to produce TRS from G. verrucosa for bio-refinery applications. Highlights Recombinant agarases were used to produce reducing sugar from Gracilaria verrucosa. Three agarases from Saccharophagus degradans were successfully expressed in E. coli. A higher yield (47.4%) of reducing sugar was obtained than with commercial enzyme. This study proposes combined hydrolysis using weak acids and recombinant enzymes. Graphical abstract [DISPLAY OMISSION]

Keyword

참고문헌 (29)

Bioresour. Technol. Jeong 181 1 2015 10.1016/j.biortech.2015.01.038 Application of solid-acid catalyst and marine macro-algae Gracilaria verrucosa to production of fermentable sugars

상세보기
Renew. Sust. Energ. Rev. Saxena 13 167 2009 10.1016/j.rser.2007.07.011 Biomass-based energy fuel through biochemical routes: a review

상세보기
Renew. Energy Hoekman 34 14 2009 10.1016/j.renene.2008.04.030 Biofuels in the U.S. - challenges and opportunities

상세보기
Bioprocess Biosyst. Eng. Jang 35 11 2012 10.1007/s00449-011-0611-2 Optimization of saccharification and ethanol production by simultaneous saccharification and fermentation (SSF) from seaweed Saccharina japonica

상세보기
Algal Res. Jiang 14 48 2016 10.1016/j.algal.2016.01.001 Macroalgae (seaweed) for liquid transportation biofuel production: what is next?

상세보기
J. Appl. Phycol. Meinita 25 1957 2013 10.1007/s10811-013-0041-4 Comparison of agarophytes (Gelidium, Gracilaria, and Gracilariopsis), as potential resources for bioethanol production

상세보기
Appl. Microbiol. Biotechnol. Chi 94 917 2012 10.1007/s00253-012-4023-2 Agar degradation by microorganisms and agar-degrading enzymes

상세보기
Appl. Environ. Microbiol. Ekborg 72 3396 2006 10.1128/AEM.72.5.3396-3405.2006 Genomic and proteomic analyses of the agarolytic system expressed by Saccharophagus degradans 2-40

상세보기
Cellul. Chem. Technol. Brosse 44 71 2010 Dilute sulfuric acid and ethanol organosolv pretreatment of Miscanthus×giganteus
Bioresour. Technol. Park 108 83 2011 10.1016/j.biortech.2011.12.065 Use of Gelidium amansii as a promising resource for bioethanol: a practical approach for continuous dilute-acid hydrolysis and fermentation

상세보기
Bioresour. Technol. Kim 196 634 2015 10.1016/j.biortech.2015.08.016 High-yield production of reducing sugars from Gracilaria verrucosa by acid and enzymatic hydrolysis processes

상세보기
Anal. Chem. Miller 31 1959 426 1959 10.1021/ac60147a030 Use of dinitrosalicylic acid reagent for determination of reducing sugar

상세보기
Appl. Microbiol. Biotechnol. Kim 86 227 2010 10.1007/s00253-009-2256-5 Overexpression and molecular characterization of Aga50D from Saccharophagus degradans 2-40: an exo-type β-agarase producing neoagarobiose

상세보기
Bioresour. Technol. Kim 136 582 2013 10.1016/j.biortech.2013.03.038 High temperature and low acid pretreatment and agarase treatment of agarose for the production of sugar and ethanol from red seaweed biomass

상세보기
Process Biochem. Yun 46 88 2011 10.1016/j.procbio.2010.07.019 Production of 3,6-anhydro-l-galactose from agarose by agarolytic enzymes of Saccharophagus degradans 2-40

상세보기
Bioresour. Technol. Gao 179 490 2015 10.1016/j.biortech.2014.12.040 Enhanced hydrolysis of Macrocystis pyrifera by integrated hydroxyl radicals and hot water pretreatment

상세보기
Bioresour. Technol. Lee 127 119 2013 10.1016/j.biortech.2012.09.122 Ethanol production from Saccharina japonica using an optimized extremely low acid pretreatment followed by simultaneous saccharification and fermentation

상세보기
Bioprocess Biosyst. Eng. Jeong 38 207 2015 10.1007/s00449-014-1259-5 Conversion of red-algae Gracilaria verrucosa to sugars, levulinic acid and 5-hydroxymethylfurfural

상세보기
J. Taiwan Fish. Res. Wu 18 65 2010 Bioethanol from acid-hydrolyzed Gacilaria
Bioprocess Biosyst. Eng. Kwon 39 1173 2016 10.1007/s00449-016-1593-x Potential of phosphoric acid-catalyzed pretreatment and subsequent enzymatic hydrolysis for reducing sugar production from Gracilaria verrucosa

상세보기
Bioresour. Technol. Wu 156 123 2014 10.1016/j.biortech.2014.01.024 Sequential acid and enzymatic hydrolysis in situ and bioethanol production from Gracilaria biomass

상세보기
Bioresour. Technol. Jeong 116 435 2012 10.1016/j.biortech.2012.03.104 Behaviors of glucose decomposition during acid-catalyzed hydrothermal hydrolysis of pretreated Gelidium amansii

상세보기
Algal Res. Kwon 13 293 2016 10.1016/j.algal.2015.12.011 Production of sugars from macro-algae Gracilaria verrucosa using combined process of citric acid-catalyzed pretreatment and enzymatic hydrolysis

상세보기
Bioresources Feng 7 3755 2012 10.15376/biores.7.3.3755-3766 Effect of inhibitors on enzymatic hydrolysis and simultaneous saccharification fermentation for lactic acid production from steam explosion pretreated Lespedeza stalks

상세보기
Biomass Bioenergy Scordia 59 540 2013 10.1016/j.biombioe.2013.09.011 Effectiveness of dilute oxalic acid pretreatment of Miscanthus×giganteus biomass for ethanol production

상세보기
Food Hydrocoll. Abd-Rahim 42 309 2014 10.1016/j.foodhyd.2014.05.017 Production of high yield sugars from Kappaphycus alvarezii using combined methods of chemical and enzymatic hydrolysis

상세보기
Bioresour. Technol. Ra 140 421 2013 10.1016/j.biortech.2013.04.122 Biotransformation of 5-hydroxymethylfurfural (HMF) by Scheffersomyces stipitis during ethanol fermentation of hydrolysate of the seaweed Gelidium amansii

상세보기
Microbiol. Biotechnol. Lett. Ra 43 9 2015 10.4014/mbl.1410.10007 Thermal acid hydrolysis pretreatment, enzymatic saccharification and ethanol fermentation from red seaweed, Gracilaria verrucosa

원문보기 상세보기
Process Biochem. Seo 51 759 2016 10.1016/j.procbio.2016.03.011 Agarose hydrolysis by two-stage enzymatic process and bioethanol production from the hydrolysate

상세보기

LOADING...

활용도 분석정보

상세보기

다운로드

내보내기

활용도 Top5 논문

해당 논문의 주제분야에서 활용도가 높은 상위 5개 콘텐츠를 보여줍니다.
더보기 버튼을 클릭하시면 더 많은 관련자료를 살펴볼 수 있습니다.

내보내기 구분	파일저장 인쇄 메일전송
구성항목	기본정보 상세정보 관리번호, 논문명, 저널/프로시딩명, 저자 , 발행년, 권, 호, 시작페이지, 끝페이지, 발행기관 관리번호, 논문명, 대등논문명, 저자 , 저널/프로시딩명, 발행기관, 발행년, 발행언어, 권, 호, 시작페이지, 끝페이지, ISBN, ISSN, 주제분야, 키워드, 초록(한글), 초록(영문), 저자(소속기관)
저장형식	Text(ASCII format) Excel format RefWorks Direct Export RIS format (for Reference Manager, ProCite, EndNote), Scholar's Aids, Mendeley
메일정보	받는사람 (필수) @ 보내는사람 (선택) @ 제목 내용 KISTI 검색결과 이메일 서비스
안내	총 건의 자료가 검색되었습니다. 다운받으실 자료의 인덱스를 입력하세요. (1-10,000) 검색결과의 순서대로 최대 10,000건 까지 다운로드가 가능합니다. 데이타가 많을 경우 속도가 느려질 수 있습니다.(최대 2~3분 소요) 다운로드 파일은 UTF-8 형태로 저장됩니다. 파일의 내용이 제대로 보이지 않을실 때는 웹브라우저 상단의 보기 -> 인코딩 -> 자동선택 여부를 확인하십시오. ~ Text(ASCII format) Excel format

연합인증

[해외논문] Recombinant agarase increases the production of reducing sugars from HCl-treated Gracilaria verrucosa, a red algae

Abstract ▼ AI-Helper

Keyword

참고문헌 (29)

이 논문을 인용한 문헌

연구과제 타임라인

활용도 분석정보

활용도 Top5 논문

관련 콘텐츠

원문 URL 링크

이 논문과 함께 이용한 콘텐츠

AI-Helper ※ AI-Helper는 오픈소스 모델을 사용합니다.

선택된 텍스트

연합인증

[해외논문] Recombinant agarase increases the production of reducing sugars from HCl-treated Gracilaria verrucosa, a red algae

Abstract ▼ AI-Helper

Keyword

참고문헌 (29)

이 논문을 인용한 문헌

연구과제 타임라인

전체(0) 논문(0) 특허(0) 보고서(0)

전체(0) 논문(0) 특허(0) 보고서(0)

활용도 분석정보

활용도 Top5 논문 더보기

관련 콘텐츠

원문 URL 링크

이 논문과 함께 이용한 콘텐츠

AI-Helper ※ AI-Helper는 오픈소스 모델을 사용합니다.

선택된 텍스트

활용도 Top5 논문