[논문]Polyphenolic Profile, Antioxidant and Anti-Inflammatory Activity of Eastern Teaberry ( Gaultheria procumbens L.) Leaf Extracts

Michel, Piotr; Dobrowolska, Anna; Kicel, Agnieszka; Owczarek, Aleksandra; Bazylko, Agnieszka; Granica, Sebastian; Piwowarski, Jakub P.; Olszewska, Monika A.

doi:10.3390/molecules191220498

[해외논문] Polyphenolic Profile, Antioxidant and Anti-Inflammatory Activity of Eastern Teaberry ( Gaultheria procumbens L.) Leaf Extracts 원문보기

Molecules a journal of synthetic chemistry and natural product chemistry, v.19 no.12, 2014년, pp.20498 - 20520

Michel, Piotr (Department of Pharmacognosy, Faculty of Pharmacy, Medical University of Lodz, 1 Muszyń) , Dobrowolska, Anna (skiego St., Lodz 90-151, Poland) , Kicel, Agnieszka (E-Mails: piotr.michel@umed.lodz.pl (P.M.)) , Owczarek, Aleksandra (a.m.dobrowolska@wp.pl (A.D.)) , Bazylko, Agnieszka (agnieszka.kicel@umed.lodz.pl (A.K.)) , Granica, Sebastian (aleksandra.owczarek@umed.lodz.pl (A.O.)) , Piwowarski, Jakub P. (Department of Pharmacognosy, Faculty of Pharmacy, Medical University of Lodz, 1 Muszyń) , Olszewska, Monika A. (skiego St., Lodz 90-151, Poland)

Abstract ▼ AI-Helper

Dry leaf extracts of eastern teaberry (Gaultheria procumbens L.) were evaluated as a source of bioactive phytocompounds through systematic activity testing and phytochemical profiling. The antioxidant efficiency was tested using five complementary in vitro models (DPPH; FRAP; linoleic acid (LA) peroxidation assay; O2•− and H2O2 scavenging tests) in parallel with standard antioxidants. The 75% methanol extract and its diethyl ether, ethyl acetate (EAF), n-butanol and water fractions exhibited the dose-dependent responses in all assays, with the highest capacities found for EAF (DPPH EC50 = 2.9 μg/mL; FRAP = 12.8 mmol Fe2+/g; IC50 for LA-peroxidation = 123.9 μg/mL; O2•− SC50 = 3.9 μg/mL; H2O2 SC50 = 7.2 μg/mL). The EAF had also the highest anti-inflammatory activity in the inhibition tests of lipoxygenase and hyaluronidase (60.14% and 21.83% effects, respectively, at the concentration of 100 μg/mL). Activity parameters of the extracts correlated strongly with the levels of total phenolics (72.4–270.7 mg GAE/g), procyanidins, and phenolic acids, whereas for flavonoids only moderate effects were observed. Comprehensive UHPLC-PDA-ESI-MS3 and HPLC-PDA studies led to the identification of 35 polyphenols with a procyanidin A-type trimer, quercetin 3-O-glucuronide, isomers of caffeoylquinic acids, and (‒)-epicatechin being the dominant components. Significant activity levels, high phenolic contents and high extraction yields (39.4%–42.5% DW for defatted and crude methanol extracts, respectively) indicate the value of eastern teaberry leaves as bioactive products.

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