[논문]Atopic dermatitis-like skin lesions are suppressed in fat-1 transgenic mice through the inhibition of inflammasomes

Jang, Hyun-Young; Koo, Jeung-Hyun; Lee, Sang-Myeong; Park, Byung-Hyun

doi:10.1038/s12276-018-0104-3

[국내논문] Atopic dermatitis-like skin lesions are suppressed in fat-1 transgenic mice through the inhibition of inflammasomes 원문보기

Experimental & molecular medicine : EMM, v.50 no.6, 2018년, pp.1 - 9

Jang, Hyun-Young (Department of Biochemistry, Chonbuk National University Medical School, Jeonju, Jeonbuk 54896 Republic of Korea) , Koo, Jeung-Hyun (Department of Biochemistry, Chonbuk National University Medical School, Jeonju, Jeonbuk 54896 Republic of Korea) , Lee, Sang-Myeong (Division of Biotechnology, College of Environmental and Bioresource Sciences, Chonbuk National University, Iksan, Jeonbuk 54596 Republic of Korea) , Park, Byung-Hyun (Department of Biochemistry, Chonbuk National University Medical School, Jeonju, Jeonbuk 54896 Republic of Korea)

Abstract ▼ AI-Helper

Previous clinical trials have addressed the beneficial effects of fish oil supplementation on atopic dermatitis. Recently, we reported that fat-1 mice, which can convert n-6 to n-3 polyunsaturated fatty acids (PUFAs), are protected against allergic airway inflammation because their Th2 immune responses are suppressed. Here, we examined the effects of endogenously synthesized n-3 PUFAs on atopic dermatitis, a representative Th2-dominant allergic inflammatory disease. Mouse models of atopic dermatitis-like skin lesions were prepared by epicutaneous application of 2,4-dinitrochlorobenzene (DNCB) or house dust mite (HDM) extract to the ears. DNCB-treated fat-1 mice exhibited markedly reduced epidermal thickening, lower mast cell infiltration, and lower serum IgE and histamine compared with wild-type mice. The draining lymph nodes of fat-1 mice were substantially smaller and contained significantly smaller proportions of activated CD4+ T cells and IL-4-producing Th2 cells than those of wild-type mice. Consistent with these findings, the mRNA levels of Th2 cytokines were significantly decreased in DNCB-sensitized skin lesions of fat-1 mice. Lastly, inflammasome activation, IL-1β production, and pyroptotic cell injury were suppressed in fat-1 mice. Similar results were observed in HDM-challenged fat-1 mice. This study confirms the results of previous clinical studies and suggests fish oil supplementation as a therapeutic strategy for atopic dermatitis-like skin lesions.Skin disease: Omega-3 fatty acid reduces inflammationA component of fish oil reduces the severity of atopic dermatitis in mice by dampening the immune response. Omega-3 fatty acids found in fish oil are essential nutrients that humans and other mammals need but cannot produce. Byung-Hyun Park at Chonbuk National University Medical School, Jeonju, South Korea, and colleagues show that genetically engineered mice expressing a gene which enables them to synthesize their own omega-3 fatty acids are protected from atopic dermatitis. Following exposure to a dermatitis-inducing chemical or house dust mite extract these mice developed smaller skin lesions and mounted a weaker inflammatory response than wild-type mice. This study confirms the anti-inflammatory effects of omega-3 fatty acids and highlights the use of fish oil supplements as a useful strategy for the treatment of atopic dermatitis and potentially other chronic inflammatory diseases.

참고문헌 (44)

1. Liang Y Chang C Lu Q The genetics and epigenetics of atopic dermatitis-filaggrin and other polymorphisms Clin. Rev. Allergy Immunol. 2016 51 315 328 26385242

상세보기
2. Brandt EB Sivaprasad U Th2 cytokines and atopic dermatitis J. Clin. Cell Immunol. 2011 2 110 21994899
3. Kabashima K New concept of the pathogenesis of atopic dermatitis: interplay among the barrier, allergy, and pruritus as a trinity J. Dermatol. Sci. 2013 70 3 11 23473856

상세보기
4. Weidinger S Novak N Atopic dermatitis Lancet 2016 387 1109 1122 26377142
5. Ito T TSLP-activated dendritic cells induce an inflammatory T helper type 2 cell response through OX40 ligand J. Exp. Med 2005 202 1213 1223 16275760

상세보기
6. Soumelis V Human epithelial cells trigger dendritic cell mediated allergic inflammation by producing TSLP Nat. Immunol. 2002 3 673 680 12055625

상세보기
7. Tatsuno K Fujiyama T Yamaguchi H Waki M Tokura Y TSLP directly interacts with skin-homing Th2 cells highly expressing its receptor to enhance IL-4 production in atopic dermatitis J. Invest Dermatol. 2015 135 3017 3024 26288354

상세보기
8. Grewe M Analysis of the cytokine pattern expressed in situ in inhalant allergen patch test reactions of atopic dermatitis patients J. Invest Dermatol. 1995 105 407 410 7665922

상세보기
9. Jiao D NOD2 and TLR2 ligands trigger the activation of basophils and eosinophils by interacting with dermal fibroblasts in atopic dermatitis-like skin inflammation Cell Mol. Immunol. 2016 13 535 550 26388234
10. Arend WP Palmer G Gabay C IL-1, IL-18, and IL-33 families of cytokines Immunol. Rev. 2008 223 20 38 18613828

상세보기
11. Macaluso F Polymorphisms in NACHT-LRR (NLR) genes in atopic dermatitis Exp. Dermatol. 2007 16 692 698 17620097

상세보기
12. Zhang Q Fan HW Zhang JZ Wang YM Xing HJ NLRP3rs35829419 polymorphism is associated with increased susceptibility to multiple diseases in humans Genet Mol. Res 2015 14 13968 13980 26535712

상세보기
13. Bivik C Genetic variation in the inflammasome and atopic dermatitis susceptibility J. Invest Dermatol. 2013 133 2486 2489 23563199

상세보기
14. Belloni B Low-dose anti-IgE therapy in patients with atopic eczema with high serum IgE levels J. Allergy Clin. Immunol. 2007 120 1223 1225 17936892

상세보기
15. He A Feldman SR Fleischer AB Trends in atopic dermatitis management: comparison of 1990-1997 to 2003-2012 J. Drugs Dermatol. 2018 17 135 140 29462220
16. Chong M Fonacier L Treatment of eczema: corticosteroids and beyond Clin. Rev. Allergy Immunol. 2016 51 249 262 25869743

상세보기
17. Hageman JH The impact of dietary long-chain polyunsaturated fatty acids on respiratory illness in infants and children Curr. Allergy Asthma Rep. 2012 12 564 573 23001718
18. Faber J Supplementation with a fish oil-enriched, high-protein medical food leads to rapid incorporation of EPA into white blood cells and modulates immune responses within one week in healthy men and women J. Nutr. 2011 141 964 970 21430245

상세보기
19. Jang HY Lim K Lee SM Park BH Effects of n-3 PUFA on the CD4 + type 2 helper T-cell-mediated immune responses in Fat-1 mice Mol. Nutr. Food Res 2014 58 365 375 24019303

상세보기
20. Brown AL Omega-3 fatty acids ameliorate atherosclerosis by favorably altering monocyte subsets and limiting monocyte recruitment to aortic lesions Arterioscler. Thromb. Vasc. Biol. 2012 32 2122 2130 22814747

상세보기
21. Song MY Enhanced M2 macrophage polarization in high n-3 polyunsaturated fatty acid transgenic mice fed a high-fat diet Mol. Nutr. Food Res 2016 60 2481 2492 27306613

상세보기
22. Teague H Rockett BD Harris M Brown DA Shaikh SR Dendritic cell activation, phagocytosis and CD69 expression on cognate T cells are suppressed by n-3 long-chain polyunsaturated fatty acids Immunology 2013 139 386 394 23373457

상세보기
23. Kang JX Wang J Wu L Kang ZB Transgenic mice: fat-1 mice convert n-6 to n-3 fatty acids Nature 2004 427 504 14765186

상세보기
24. Weylandt KH Reduction of inflammation and chronic tissue damage by omega-3 fatty acids in fat-1 transgenic mice with pancreatitis Biochim Biophys. Acta 2008 1782 634 641 18832028
25. Woo SJ Endogenous conversion of n-6 to n-3 polyunsaturated fatty acids attenuates K/BxN serum-transfer arthritis in fat-1 mice J. Nutr. Biochem 2015 26 713 720 25910895

상세보기
26. Kurashima Y The enzyme Cyp26b1 mediates inhibition of mast cell activation by fibroblasts to maintain skin-barrier homeostasis Immunity 2014 40 530 541 24726878

상세보기
27. Dai X Mite allergen is a danger signal for the skin via activation of inflammasome in keratinocytes J. Allergy Clin. Immunol. 2011 127 806 14. e1-4 21272927

상세보기
28. Iversen L Johansen C Inflammasomes and inflammatory caspases in skin inflammation Expert Rev. Mol. Diagn. 2008 8 697 705 18999922

상세보기
29. Miao EA Rajan JV Aderem A Caspase-1-induced pyroptotic cell death Immunol. Rev. 2011 243 206 214 21884178

상세보기
30. Furuhjelm C Allergic disease in infants up to 2 years of age in relation to plasma omega-3 fatty acids and maternal fish oil supplementation in pregnancy and lactation Pediatr. Allergy Immunol. 2011 22 505 514 21332799

상세보기
31. Denburg JA Fish oil supplementation in pregnancy modifies neonatal progenitors at birth in infants at risk of atopy Pediatr. Res 2005 57 276 281 15585690
32. Birch EE The impact of early nutrition on incidence of allergic manifestations and common respiratory illnesses in children J. Pediatr. 2010 156 902 906 20227721
33. McCusker MM Grant-Kels JM Healing fats of the skin: the structural and immunologic roles of the omega-6 and omega-3 fatty acids Clin. Dermatol. 2010 28 440 451 20620762
34. Han SC Docosahexaenoic acid alleviates atopic dermatitis by generating Tregs and IL-10/TGF-β-modified macrophages via a TGF-β-dependent mechanism J. Invest Dermatol. 2015 135 1556 1564 25405323
35. Park BK Omega-3 fatty acids suppress Th2-associated cytokine gene expressions and GATA transcription factors in mast cells J. Nutr. Biochem 2013 24 868 876 22902330

상세보기
36. Herberth G Endogenous metabolites and inflammasome activity in early childhood and links to respiratory diseases J. Allergy Clin. Immunol. 2015 136 495 497 25754623

상세보기
37. Wen H Fatty acid-induced NLRP3-ASC inflammasome activation interferes with insulin signaling Nat. Immunol. 2011 12 408 415 21478880

상세보기
38. Reynolds CM Dietary saturated fatty acids prime the NLRP3 inflammasome via TLR4 in dendritic cells-implications for diet-induced insulin resistance Mol. Nutr. Food Res 2012 56 1212 1222 22700321

상세보기
39. Yan Y Omega-3 fatty acids prevent inflammation and metabolic disorder through inhibition of NLRP3 inflammasome activation Immunity 2013 38 1154 1163 23809162

상세보기
40. Finucane OM Monounsaturated fatty acid-enriched high-fat diets impede adipose NLRP3 inflammasome-mediated IL-1β secretion and insulin resistance despite obesity Diabetes 2015 64 2116 2128 25626736
41. Bauernfeind FG Cutting edge: NF-κB activating pattern recognition and cytokine receptors license NLRP3 inflammasome activation by regulating NLRP3 expression J. Immunol. 2009 183 787 791 19570822
42. Guttman-Yassky E Krueger JG Atopic dermatitis and psoriasis: two different immune diseases or one spectrum? Curr. Opin. Immunol. 2017 48 68 73 28869867

상세보기
43. Di Cesare A Di Meglio P Nestle FO A role for Th17 cells in the immunopathogenesis of atopic dermatitis? J. Invest Dermatol. 2008 128 2569 2571 18927538

상세보기
44. Pennino D IL-17 amplifies human contact hypersensitivity by licensing hapten nonspecific Th1 cells to kill autologous keratinocytes J. Immunol. 2010 184 4880 4888 20357258

상세보기

LOADING...

표제어: PCR

동의어: Packet Collision Rate

용어 설명 출처 목록 (6)

용어 설명: PCR은 세균 특이성이 있는 primer를 이용하여 적은 수의 세균이 있을지라도 쉽게 검출할 수 있는 유용한 방법이며, 이를 이용하여 구강 내 치면세균막이나 타액에서 직접 세균을 검출할 수 있게 되었다[8].

내보내기 구분	파일저장 인쇄 메일전송
구성항목	기본정보 상세정보 관리번호, 논문명, 저널/프로시딩명, 저자 , 발행년, 권, 호, 시작페이지, 끝페이지, 발행기관 관리번호, 논문명, 대등논문명, 저자 , 저널/프로시딩명, 발행기관, 발행년, 발행언어, 권, 호, 시작페이지, 끝페이지, ISBN, ISSN, 주제분야, 키워드, 초록(한글), 초록(영문), 저자(소속기관)
저장형식	Text(ASCII format) Excel format RefWorks Direct Export RIS format (for Reference Manager, ProCite, EndNote), Scholar's Aids, Mendeley
메일정보	받는사람 (필수) @ 보내는사람 (선택) @ 제목 내용 KISTI 검색결과 이메일 서비스
안내	총 건의 자료가 검색되었습니다. 다운받으실 자료의 인덱스를 입력하세요. (1-10,000) 검색결과의 순서대로 최대 10,000건 까지 다운로드가 가능합니다. 데이타가 많을 경우 속도가 느려질 수 있습니다.(최대 2~3분 소요) 다운로드 파일은 UTF-8 형태로 저장됩니다. 파일의 내용이 제대로 보이지 않을실 때는 웹브라우저 상단의 보기 -> 인코딩 -> 자동선택 여부를 확인하십시오. ~ Text(ASCII format) Excel format

연합인증

[국내논문] Atopic dermatitis-like skin lesions are suppressed in fat-1 transgenic mice through the inhibition of inflammasomes 원문보기

Abstract ▼ AI-Helper

참고문헌 (44)

이 논문을 인용한 문헌

연구과제 타임라인

관련 콘텐츠

원문 보기

원문 URL 링크

오픈액세스(OA) 유형

이 논문과 함께 이용한 콘텐츠

AI-Helper ※ AI-Helper는 오픈소스 모델을 사용합니다.

선택된 텍스트

연합인증

[국내논문] Atopic dermatitis-like skin lesions are suppressed in fat-1 transgenic mice through the inhibition of inflammasomes 원문보기

Abstract ▼ AI-Helper

참고문헌 (44)

이 논문을 인용한 문헌

연구과제 타임라인

전체(0) 논문(0) 특허(0) 보고서(0)

전체(0) 논문(0) 특허(0) 보고서(0)

관련 콘텐츠

원문 보기

원문 URL 링크

오픈액세스(OA) 유형

이 논문과 함께 이용한 콘텐츠

AI-Helper ※ AI-Helper는 오픈소스 모델을 사용합니다.

선택된 텍스트