[논문]Comparison of Phenotypic and Functional Characteristics Between Canine Non-B, Non-T Natural Killer Lymphocytes and CD3 + CD5 dim CD21 − Cytotoxic Large Granular Lymphocytes

Lee, Soo-Hyeon; Shin, Dong-Jun; Kim, Yoseop; Kim, Cheol-Jung; Lee, Je-Jung; Yoon, Mee Sun; Uong, Tung Nguyen Thanh; Yu, Dohyeon; Jung, Ji-Youn; Cho, Duck; Jung, Bock-Gie; Kim, Sang-Ki; Suh, Guk-Hyun

doi:10.3389/fimmu.2018.00841

Comparison of Phenotypic and Functional Characteristics Between Canine Non-B, Non-T Natural Killer Lymphocytes and CD3 + CD5 dim CD21 − Cytotoxic Large Granular Lymphocytes 원문보기

Frontiers in immunology, v.9, 2018년, pp.841 -

Lee, Soo-Hyeon (Department of Integrated Life Science and Technology, Kongju National University , Yesan-gun , South Korea) , Shin, Dong-Jun (Department of Laboratory and Companion Animal Science, College of Industrial Science, Kongju National University , Yesan-gun , South Korea) , Kim, Yoseop (Department of Laboratory and Companion Animal Science, College of Industrial Science, Kongju National University , Yesan-gun , South Korea) , Kim, Cheol-Jung (Department of Laboratory and Companion Animal Science, College of Industrial Science, Kongju National University , Yesan-gun , South Korea) , Lee, Je-Jung (Department of Hemotology-Oncology, Chonnam National University Hwasun Hospital , Hwasun , South Korea) , Yoon, Mee Sun (Department of Radiation Oncology, Chonnam National University Hwasun Hospital, Chonnam National University Medical School , Gwangju , South Korea) , Uong, Tung Nguyen Thanh (Department of Radiation) , Yu, Dohyeon , Jung, Ji-Youn , Cho, Duck , Jung, Bock-Gie , Kim, Sang-Ki , Suh, Guk-Hyun

Abstract ▼ AI-Helper

Natural killer (NK) cells play a pivotal role in the immune response against infections and malignant transformation, and adopted transfer of NK cells is thought to be a promising therapeutic approach for cancer patients. Previous reports describing the phenotypic features of canine NK cells have produced inconsistent results. Canine NK cells are still defined as non-B and non-T (CD3−CD21−) large granular lymphocytes. However, a few reports have demonstrated that canine NK cells share the phenotypic characteristics of T lymphocytes, and that CD3+CD5dimCD21− lymphocytes are putative canine NK cells. Based on our previous reports, we hypothesized that phenotypic modulation could occur between these two populations during activation. In this study, we investigated the phenotypic and functional differences between CD3+CD5dimCD21− (cytotoxic large granular lymphocytes) and CD3−CD5−CD21− NK lymphocytes before and after culture of peripheral blood mononuclear cells isolated from normal dogs. The results of this study show that CD3+CD5dimCD21− lymphocytes can be differentiated into non-B, non-T NK (CD3−CD5−CD21−TCRαβ−TCRγδ−GranzymeB+) lymphocytes through phenotypic modulation in response to cytokine stimulation. In vitro studies of purified CD3+CD5dimCD21− cells showed that CD3−CD5−CD21− cells are derived from CD3+CD5dimCD21− cells through phenotypic modulation. CD3+CD5dimCD21− cells share more NK cell functional characteristics compared with CD3−CD5−CD21− cells, including the expression of T-box transcription factors (Eomes, T-bet), the production of granzyme B and interferon-γ, and the expression of NK cell-related molecular receptors such as NKG2D and NKp30. In conclusion, the results of this study suggest that CD3+CD5dimCD21− and CD3−CD5−CD21− cells both contain a subset of putative NK cells, and the difference between the two populations may be due to the degree of maturation.

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참고문헌 (48)

1 Morice WG . The immunophenotypic attributes of NK cells and NK-cell lineage lymphoproliferative disorders . Am J Clin Pathol ( 2007 ) 127 ( 6 ): 881 – 6 . 10.1309/Q49CRJ030L22MHLF 17509985

상세보기
2 Vivier E Tomasello E Baratin M Walzer T Ugolini S . Functions of natural killer cells . Nat Immunol ( 2008 ) 9 ( 5 ): 503 – 10 . 10.1038/ni1582 18425107

상세보기
3 Seidel UJ Schlegel P Lang P . Natural killer cell mediated antibody-dependent cellular cytotoxicity in tumor immunotherapy with therapeutic antibodies . Front Immunol ( 2013 ) 4 : 76 . 10.3389/fimmu.2013.00076 23543707

상세보기
4 Ljunggren HG Malmberg KJ . Prospects for the use of NK cells in immunotherapy of human cancer . Nat Rev Immunol ( 2007 ) 7 ( 5 ): 329 – 39 . 10.1038/nri2073 17438573

상세보기
5 McKenna DH Kadidlo DM Cooley S Miller JS . Clinical production and therapeutic applications of alloreactive natural killer cells . Methods Mol Biol ( 2012 ) 882 : 491 – 507 . 10.1007/978-1-61779-842-9_28 22665252

상세보기
6 Iliopoulou EG Kountourakis P Karamouzis MV Doufexis D Ardavanis A Baxevanis CN A phase I trial of adoptive transfer of allogeneic natural killer cells in patients with advanced non-small cell lung cancer . Cancer Immunol Immunother ( 2010 ) 59 ( 12 ): 1781 – 9 . 10.1007/s00262-010-0904-3 20703455

상세보기
7 Chouaib S Pittari G Nanbakhsh A El Ayoubi H Amsellem S Bourhis JH Improving the outcome of leukemia by natural killer cell-based immunotherapeutic strategies . Front Immunol ( 2014 ) 5 : 95 . 10.3389/fimmu.2014.00095 24672522

상세보기
8 Mair KH Mullebner A Essler SE Duvigneau JC Storset AK Saalmuller A Porcine CD8alphadim/-NKp46high NK cells are in a highly activated state . Vet Res ( 2013 ) 44 : 13 10.1186/1297-9716-44-13 23452562

상세보기
9 Graham EM Thom ML Howard CJ Boysen P Storset AK Sopp P Natural killer cell number and phenotype in bovine peripheral blood is influenced by age . Vet Immunol Immunopathol ( 2009 ) 132 ( 2–4 ): 101 – 8 . 10.1016/j.vetimm.2009.05.002 19525013

상세보기
10 Inngjerdingen M Kveberg L Naper C Vaage JT . Natural killer cell subsets in man and rodents . Tissue Antigens ( 2011 ) 78 ( 2 ): 81 – 8 . 10.1111/j.1399-0039.2011.01714.x 21726202

상세보기
11 Walter L . Major histocompatibility complex class-I-interacting natural killer cell receptors of nonhuman primates . J Innate Immun ( 2011 ) 3 ( 3 ): 236 – 41 . 10.1159/000323932 21411971

상세보기
12 Caligiuri MA . Human natural killer cells . Blood ( 2008 ) 112 ( 3 ): 461 – 9 . 10.1182/blood-2007-09-077438 18650461

상세보기
13 Sojka DK Tian Z Yokoyama WM . Tissue-resident natural killer cells and their potential diversity . Semin Immunol ( 2014 ) 26 ( 2 ): 127 – 31 . 10.1016/j.smim.2014.01.010 24548893

상세보기
14 Grondahl-Rosado C Bonsdorff TB Brun-Hansen HC Storset AK . NCR1+ cells in dogs show phenotypic characteristics of natural killer cells . Vet Res Commun ( 2015 ) 39 ( 1 ): 19 – 30 . 10.1007/s11259-014-9624-z 25434421

상세보기
15 Grondahl-Rosado C Boysen P Johansen GM Brun-Hansen H Storset AK . NCR1 is an activating receptor expressed on a subset of canine NK cells . Vet Immunol Immunopathol ( 2016 ) 177 : 7 – 15 . 10.1016/j.vetimm.2016.05.001 27436439

상세보기
16 Foltz JA Somanchi SS Yang Y Aquino-Lopez A Bishop EE Lee DA . NCR1 expression identifies canine natural killer cell subsets with phenotypic similarity to human natural killer cells . Front Immunol ( 2016 ) 7 : 521 . 10.3389/fimmu.2016.00521 27933061

상세보기
17 Shin DJ Park JY Jang YY Lee JJ Lee YK Shin MG Ex vivo expansion of canine cytotoxic large granular lymphocytes exhibiting characteristics of natural killer cells . Vet Immunol Immunopathol ( 2013 ) 153 ( 3–4 ): 249 – 59 . 10.1016/j.vetimm.2013.03.006 23548866

상세보기
18 Michael HT Ito D McCullar V Zhang B Miller JS Modiano JF . Isolation and characterization of canine natural killer cells . Vet Immunol Immunopathol ( 2013 ) 155 ( 3 ): 211 – 7 . 10.1016/j.vetimm.2013.06.013 23876304

상세보기
19 Ringler SS Krakowka S . Cell surface markers of the canine natural killer (NK) cell . Vet Immunol Immunopathol ( 1985 ) 9 ( 1 ): 1 – 11 . 10.1016/0165-2427(85)90125-4 2862730

상세보기
20 Huang YC Hung SW Jan TR Liao KW Cheng CH Wang YS CD5-low expression lymphocytes in canine peripheral blood show characteristics of natural killer cells . J Leukoc Biol ( 2008 ) 84 ( 6 ): 1501 – 10 . 10.1189/jlb.0408255 18708592

상세보기
21 Loughran TP Jr Deeg HJ Storb R . Morphologic and phenotypic analysis of canine natural killer cells: evidence for T-cell lineage . Cell Immunol ( 1985 ) 95 ( 2 ): 207 – 17 . 10.1016/0008-8749(85)90309-0 3876157

상세보기
22 Lin YC Huang YC Wang YS Juang RH Liao KW Chu RM . Canine CD8 T cells showing NK cytotoxic activity express mRNAs for NK cell-associated surface molecules . Vet Immunol Immunopathol ( 2010 ) 133 ( 2–4 ): 144 – 53 . 10.1016/j.vetimm.2009.07.013 19709755

상세보기
23 Nakada Y Tokumitu K Kosaka T Kuwabara M Tanaka S Koide F . Correlation between canine NK cell mediated cytotoxicity and radical production . Vet Immunol Immunopathol ( 1995 ) 45 ( 3–4 ): 285 – 95 . 10.1016/0165-2427(94)05350-2 7676611

상세보기
24 Shin DJ Lee SH Park JY Kim JS Lee JJ Suh GH Interleukin-21 induces proliferation and modulates receptor expression and effector function in canine natural killer cells . Vet Immunol Immunopathol ( 2015 ) 165 ( 1–2 ): 22 – 33 . 10.1016/j.vetimm.2015.03.004 25819349

상세보기
25 Lee SH Shin DJ Kim SK . Generation of recombinant canine interleukin-15 and evaluation of its effects on the proliferation and function of canine NK cells . Vet Immunol Immunopathol ( 2015 ) 165 ( 1–2 ): 1 – 13 . 10.1016/j.vetimm.2015.04.002 25890849

상세보기
26 Park JY Shin DJ Lee SH Lee JJ Suh GH Cho D The anti-canine distemper virus activities of ex vivo-expanded canine natural killer cells . Vet Microbiol ( 2015 ) 176 ( 3–4 ): 239 – 49 . 10.1016/j.vetmic.2015.01.021 25680810

상세보기
27 Fujisaki H Kakuda H Shimasaki N Imai C Ma J Lockey T Expansion of highly cytotoxic human natural killer cells for cancer cell therapy . Cancer Res ( 2009 ) 69 ( 9 ): 4010 – 7 . 10.1158/0008-5472.CAN-08-3712 19383914

상세보기
28 Rothe K Bismarck D Buttner M Alber G von Buttlar H Canine peripheral blood CD4(+)CD8(+) double-positive Tcell subpopulations exhibit distinct Tcell phenotypes and effector functions . Vet Immunol Immunopathol ( 2017 ) 185 : 48 – 56 . 10.1016/j.vetimm.2017.01.005 28242002

상세보기
29 Park YK Shin DJ Cho D Kim SK Lee JJ Shin MG Interleukin-21 increases direct cytotoxicity and IFN-gamma production of ex vivo expanded NK cells towards breast cancer cells . Anticancer Res ( 2012 ) 32 ( 3 ): 839 – 46 . 22399602
30 Knox JJ Cosma GL Betts MR McLane LM . Characterization of T-bet and eomes in peripheral human immune cells . Front Immunol ( 2014 ) 5 : 217 . 10.3389/fimmu.2014.00217 24860576

상세보기
31 Simonetta F Pradier A Roosnek E T-bet and eomesodermin in NK cell development, maturation, and function . Front Immunol ( 2016 ) 7 : 241 10.3389/fimmu.2016.00241 27379101

상세보기
32 Canter RJ Grossenbacher SK Foltz JA Sturgill IR Park JS Luna JI Radiotherapy enhances natural killer cell cytotoxicity and localization in pre-clinical canine sarcomas and first-in-dog clinical trial . J Immunother Cancer ( 2017 ) 5 ( 1 ): 98 . 10.1186/s40425-017-0305-7 29254507

상세보기
33 Mair KH Stadler M Talker SC Forberg H Storset AK Mullebner A Porcine CD3(+)NKp46(+) lymphocytes have NK-cell characteristics and are present in increased frequencies in the lungs of influenza-infected animals . Front Immunol ( 2016 ) 7 : 263 . 10.3389/fimmu.2016.00263 27471504

상세보기
34 Connelley TK Longhi C Burrells A Degnan K Hope J Allan AJ NKp46+ CD3+ cells: a novel nonconventional T cell subset in cattle exhibiting both NK cell and T cell features . J Immunol ( 2014 ) 192 ( 8 ): 3868 – 80 . 10.4049/jimmunol.1302464 24639352

상세보기
35 Moretta L Bottino C Pende D Mingari MC Biassoni R Moretta A . Human natural killer cells: their origin, receptors and function . Eur J Immunol ( 2002 ) 32 ( 5 ): 1205 – 11 . 10.1002/1521-4141(200205)32:5 3.0.CO;2-Y 11981807

상세보기
36 Lanier LL Chang C Spits H Phillips JH . Expression of cytoplasmic CD3 epsilon proteins in activated human adult natural killer (NK) cells and CD3 gamma, delta, epsilon complexes in fetal NK cells. Implications for the relationship of NK and T lymphocytes . J Immunol ( 1992 ) 149 ( 6 ): 1876 – 80 . 1387664
37 Meresse B Curran SA Ciszewski C Orbelyan G Setty M Bhagat G Reprogramming of CTLs into natural killer-like cells in celiac disease . J Exp Med ( 2006 ) 203 ( 5 ): 1343 – 55 . 10.1084/jem.20060028 16682498

상세보기
38 Stewart CA Walzer T Robbins SH Malissen B Vivier E Prinz I . Germ-line and rearranged Tcrd transcription distinguish bona fide NK cells and NK-like gammadelta T cells . Eur J Immunol ( 2007 ) 37 ( 6 ): 1442 – 52 . 10.1002/eji.200737354 17492716

상세보기
39 Li P Burke S Wang J Chen X Ortiz M Lee SC Reprogramming of T cells to natural killer-like cells upon Bcl11b deletion . Science ( 2010 ) 329 ( 5987 ): 85 – 9 . 10.1126/science.1188063 20538915

상세보기
40 Rabinowich H Sedlmayr P Herberman RB Whiteside TL . Increased proliferation, lytic activity, and purity of human natural killer cells cocultured with mitogen-activated feeder cells . Cell Immunol ( 1991 ) 135 ( 2 ): 454 – 70 . 10.1016/0008-8749(91)90290-R 1709827

상세보기
41 Norian R Delirezh N Azadmehr A . Evaluation of proliferation and cytokines production by mitogen-stimulated bovine peripheral blood mononuclear cells . Vet Res Forum ( 2015 ) 6 ( 4 ): 265 – 71 . 26973760
42 Katial RK Sachanandani D Pinney C Lieberman MM . Cytokine production in cell culture by peripheral blood mononuclear cells from immunocompetent hosts . Clin Diagn Lab Immunol ( 1998 ) 5 ( 1 ): 78 – 81 . 9455885

상세보기
43 Gordon SM Chaix J Rupp LJ Wu J Madera S Sun JC The transcription factors T-bet and Eomes control key checkpoints of natural killer cell maturation . Immunity ( 2012 ) 36 ( 1 ): 55 – 67 . 10.1016/j.immuni.2011.11.016 22261438

상세보기
44 Gill S Vasey AE De Souza A Baker J Smith AT Kohrt HE Rapid development of exhaustion and down-regulation of eomesodermin limit the antitumor activity of adoptively transferred murine natural killer cells . Blood ( 2012 ) 119 ( 24 ): 5758 – 68 . 10.1182/blood-2012-03-415364 22544698

상세보기
45 Simonetta F Pradier A Bosshard C Masouridi-Levrat S Chalandon Y Roosnek ENK Cell functional impairment after allogeneic hematopoietic stem cell transplantation is associated with reduced levels of T-bet and eomesodermin . J Immunol ( 2015 ) 195 ( 10 ): 4712 – 20 . 10.4049/jimmunol.1501522 26438526

상세보기
46 Malaise M Rovira J Renner P Eggenhofer E Sabet-Baktach M Lantow M KLRG1+ NK cells protect T-bet-deficient mice from pulmonary metastatic colorectal carcinoma . J Immunol ( 2014 ) 192 ( 4 ): 1954 – 61 . 10.4049/jimmunol.1300876 24415778

상세보기
47 Tachibana Y Nakano Y Nagaoka K Kikuchi M Nambo Y Haneda S Expression of endometrial immune-related genes possibly functioning during early pregnancy in the mare . J Reprod Dev ( 2013 ) 59 ( 1 ): 85 – 91 . 10.1262/jrd.2012-142 23138119

상세보기
48 Liu H Liu L Liu K Bizargity P Hancock WW Visner GA . Reduced cytotoxic function of effector CD8+ T cells is responsible for indoleamine 2,3-dioxygenase-dependent immune suppression . J Immunol ( 2009 ) 183 ( 2 ): 1022 – 31 . 10.4049/jimmunol.0900408 19564344

상세보기

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Comparison of Phenotypic and Functional Characteristics Between Canine Non-B, Non-T Natural Killer Lymphocytes and CD3 + CD5 dim CD21 − Cytotoxic Large Granular Lymphocytes 원문보기

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Comparison of Phenotypic and Functional Characteristics Between Canine Non-B, Non-T Natural Killer Lymphocytes and CD3 + CD5 dim CD21 − Cytotoxic Large Granular Lymphocytes 원문보기

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