[논문]Effect of Different Proteases on the Degree of Hydrolysis and Angiotensin I-Converting Enzyme-Inhibitory Activity in Goat and Cow Milk

Shu, Guowei; Huang, Jie; Bao, Chunju; Meng, Jiangpeng; Chen, He; Cao, Jili

doi:10.3390/biom8040101

Effect of Different Proteases on the Degree of Hydrolysis and Angiotensin I-Converting Enzyme-Inhibitory Activity in Goat and Cow Milk 원문보기

Biomolecules, v.8 no.4, 2018년, pp.101 -

Shu, Guowei (School of Food and Biological Engineering, Shaanxi University of Science and Technology, Xi’an 710021, China) , Huang, Jie (huangjie9319@gmail.com (J.H.)) , Bao, Chunju (lhm542527328@163.com (C.B.)) , Meng, Jiangpeng (chenhe419@gmail.com (H.C.)) , Chen, He (School of Food and Biological Engineering, Shaanxi University of Science and Technology, Xi’an 710021, China) , Cao, Jili (huangjie9319@gmail.com (J.H.))

Abstract ▼ AI-Helper

Angiotensin I-converting enzyme (ACE) peptides are bioactive peptides that have important value in terms of research and application in the prevention and treatment of hypertension. While widespread literature is concentrated on casein or whey protein for production of ACE-inhibitory peptides, relatively little information is available on selecting the proper proteases to hydrolyze the protein. In this study, skimmed cow and goat milk were hydrolyzed by four commercial proteases, including alkaline protease, trypsin, bromelain, and papain. Angiotensin I-converting enzyme-inhibitory peptides and degree of hydrolysis (DH) of hydrolysates were measured. Moreover, we compared the difference in ACE-inhibitory activity between cow and goat milk. The results indicated that the DH increased with the increase in hydrolysis time. The alkaline protease-treated hydrolysates exhibited the highest DH value and ACE-inhibitory activity. Additionally, the ACE-inhibitory activity of hydrolysates from goat milk was higher than that of cow milk-derived hydrolysates. Therefore, goat milk is a good source to obtain bioactive peptides with ACE-inhibitory activity, as compared with cow milk. A proper enzyme to produce ACE-inhibitory peptides is important for the development of functional milk products and will provide the theoretical basis for industrial production.

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Effect of Different Proteases on the Degree of Hydrolysis and Angiotensin I-Converting Enzyme-Inhibitory Activity in Goat and Cow Milk 원문보기

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