[논문]GC/MS-Based Metabolomics Reveals Biomarkers in Asthma Murine Model Modulated by  Opuntia humifusa

Seo, Seung-Ho; Kim, Eun-Ju; Park, Seong-Eun; Byun, Sung-Hoon; Lee, Soon-Young; Bok, So-Hyeon; Park, Dae-Hun; Son, Hong-Seok

doi:10.1155/2018/1202860

[해외논문] GC/MS-Based Metabolomics Reveals Biomarkers in Asthma Murine Model Modulated by Opuntia humifusa 원문보기

Evidence-based Complementary and Alternative Medicine : eCAM, v.2018, 2018년, pp.1202860 -

Seo, Seung-Ho (School of Korean Medicine, Dongshin University, Naju 58245, Republic of Korea) , Kim, Eun-Ju (School of Korean Medicine, Dongshin University, Naju 58245, Republic of Korea) , Park, Seong-Eun (School of Korean Medicine, Dongshin University, Naju 58245, Republic of Korea) , Byun, Sung-Hoon (Department of Oral and Maxillofacial Surgery, Gyeongsang National University Hospital, Jinju 52727, Republic of Korea) , Lee, Soon-Young (School of Korean Medicine, Dongshin University, Naju 58245, Republic of Korea) , Bok, So-Hyeon (School of Korean Medicine, Dongshin University, Naju 58245, Republic of Korea) , Park, Dae-Hun (School of Korean Medicine, Dongshin University, Naju 58245, Republic of Korea) , Son, Hong-Seok (School of Korean Medicine, Dongshin University, Naju 58245, Republic of Korea)

Abstract ▼ AI-Helper

GC/MS coupled with multivariate statistical analysis was performed to identify marker metabolites in serum of mice after healing ovalbumin- (OVA-) induced asthma using Opuntia humifusa. Principal component analysis (PCA) score plot showed separation among groups, with metabolite profiles of serum showing differences according to various treatments for the asthma murine model. Levels of stearic acid and arachidic acid were significantly lower in the serum from OVA-induced group than those from the control group. Dexamethasone treatment group was characterized by higher serum levels of urea, myristic acid, and palmitic acid along with lower levels of aspartic acid compared to OVA-induced group. O. humifusa treatment mice groups showed dose-proportional higher levels of urea and glycerol than OVA-induced group. These results highlight that GC/MS-based metabolomics is a powerful technique for identifying molecular markers of asthma.

참고문헌 (45)

1 Adamko D. J. Sykes B. D. Rowe B. H. The metabolomics of asthma: Novel diagnostic potential CHEST 2012 141 5 1295 1302 2-s2.0-84860561692 10.1378/chest.11-2028 22553262

상세보기
2 Leung T. F. Ko F. W. Wong G. W. Recent advances in asthma biomarker research Therapeutic Advances in Respiratory Disease 2013 7 5 297 308 10.1177/1753465813496863 23907809

상세보기
3 Ho W. E. Xu Y. Xu F. Metabolomics reveals altered metabolic pathways in experimental asthma American Journal of Respiratory Cell and Molecular Biology 2013 48 2 204 211 10.1165/rcmb.2012-0246OC 23144334

상세보기
4 Li X.-M. Treatment of asthma and food allergy with herbal interventions from traditional Chinese medicine Mount Sinai Journal of Medicine 2011 78 5 697 716 2-s2.0-80052783955 10.1002/msj.20294 21913200

상세보기
5 Lipworth B. J. Systemic adverse effects of inhaled corticosteroid therapy: a systematic review and meta-analysis JAMA Internal Medicine 1999 159 9 941 955 10.1001/archinte.159.9.941 2-s2.0-0033542041

상세보기
6 Roland N. J. Bhalla R. K. Earis J. The local side effects of inhaled corticosteroids: current understanding and review of the literature CHEST 2004 126 1 213 219 10.1378/chest.126.1.213 2-s2.0-3242734980 15249465

상세보기
7 Toogood J. H. Crilly R. G. Jones G. Nadeau J. Wells G. A. Effect of High-dose Inhaled Budesonide on Calcium and Phosphate Metabolism and the Risk of Osteoporosis American Review of Respiratory Disease 1988 138 1 57 61 10.1164/ajrccm/138.1.57 3202401

상세보기
8 Packe G. E. Robb O. Robins S. P. Reid D. M. Douglas J. G. Bone density in asthmatic patients taking inhaled corticosteroids: Comparison of budesonide and beclomethasone dipropionate Journal of the Royal College of Physicians 1996 30 2 128 132 2-s2.0-0029884478 8709058
9 Zöllner E. W. Hypothalamic-pituitary-adrenal axis suppression in asthmatic children on inhaled corticosteroids (Part 2) - The risk as determined by gold standard adrenal function tests: A systematic review Pediatric Allergy and Immunology 2007 18 6 469 474 2-s2.0-34547634019 10.1111/j.1399-3038.2007.00539.x 17680905

상세보기
10 Wang J. J. Rochtchina E. Tan A. G. Cumming R. G. Leeder S. R. Mitchell P. Use of Inhaled and Oral Corticosteroids and the Long-term Risk of Cataract Ophthalmology 2009 116 4 652 657 2-s2.0-63149120447 10.1016/j.ophtha.2008.12.001 19243828

상세보기
11 Cordina M. Fenech A. G. Vassallo J. Cacciottolo J. M. Anxiety and the management of asthma in an adult outpatient population Therapeutic Advances in Respiratory Disease 2009 3 5 227 233 2-s2.0-70449971740 10.1177/1753465809347038 19736295

상세보기
12 Bhalla R. K. Taylor W. Jones A. S. Roland N. J. The inflammation produced by corticosteroid inhalers in the pharynx in asthmatics Clinical Otolaryngology 2008 33 6 581 586 2-s2.0-58149109074 10.1111/j.1749-4486.2008.01837.x 19126133

상세보기
13 Zhou D.-Y. Du Q. Li R.-R. Huang M. Zhang Q. Wei G.-Z. Grape seed proanthocyanidin extract attenuates airway inflammation and hyperresponsiveness in a murine model of asthma by downregulating inducible nitric oxide synthase Planta Medica 2011 77 14 1575 1581 2-s2.0-80053304241 10.1055/s-0030-1270957 21452107

상세보기
14 Lu Y. Cai S. Nie J. The natural compound nujiangexanthone A suppresses mast cell activation and allergic asthma Biochemical Pharmacology 2015 100 61 72 2-s2.0-84947308048 10.1016/j.bcp.2015.11.004 26571438

상세보기
15 Huang M.-T. Lysz T. Ferraro T. Abidi T. F. Laskin J. D. Conney A. H. Inhibitory effects of curcumin on in vitro lipoxygenase and cyclooxygenase activities in mouse epidermis Cancer Research 1991 51 3 813 819 2-s2.0-0026029402 1899046

상세보기
16 Bauer J. Kuehnl S. Rollinger J. M. Carnosol and carnosic acids from Salvia officinalis inhibit microsomal prostaglandin E2 synthase-1 The Journal of Pharmacology and Experimental Therapeutics 2012 342 1 169 176 10.1124/jpet.112.193847 2-s2.0-84862489794 22511203

상세보기
17 Poeckel D. Greiner C. Verhoff M. Carnosic acid and carnosol potently inhibit human 5-lipoxygenase and suppress pro-inflammatory responses of stimulated human polymorphonuclear leukocytes Biochemical Pharmacology 2008 76 1 91 97 10.1016/j.bcp.2008.04.013 2-s2.0-45049087994 18508031

상세보기
18 Cho J. Y. Park S.-C. Kim T.-W. Radical scavenging and anti-inflammatory activity of extracts from Opuntia humifusa Raf. Journal of Pharmacy and Pharmacology 2006 58 1 113 119 2-s2.0-29944435604 10.1211/jpp.58.1.0014 16393471

상세보기
19 Lee KS. CS Oh. Lee KY. Antioxidative effect of the fractions extracted from a cactus Cheonnyuncho (Opuntia humifusa Korean Journal of Food Science and Technology 2005 37 3 474 478
20 Lee S. Bae C. Choi Y. Food & Nutrition Research 2017 61 1 p. 1393307 10.1080/16546628.2017.1393307

상세보기
21 Ober C. Yao T.-C. The genetics of asthma and allergic disease: A 21st century perspective Immunological Reviews 2011 242 1 10 30 10.1111/j.1600-065X.2011.01029.x 2-s2.0-79959354208 21682736

상세보기
22 Kelly R. S. Dahlin A. McGeachie M. J. Asthma Metabolomics and the Potential for Integrative Omics in Research and the Clinic CHEST 2017 151 2 262 277 10.1016/j.chest.2016.10.008 2-s2.0-85011586130 27776981

상세보기
23 Yang P. Li Z. Zhang L. Li H. Li Z. Analysis of metabonomic profiling alterations in a mouse model of colitis-associated cancer and 2-deoxy-d-glucose treatment RSC Advances 2016 6 64 58862 58870 2-s2.0-84976444893 10.1039/C6RA01718E

상세보기
24 Jansson J. Willing B. Lucio M. Metabolomics reveals metabolic biomarkers of Crohn's disease PLoS ONE 2009 4 7 2-s2.0-68149099597

상세보기
25 Radhakutty A. Mangelsdorf B. L. Drake S. M. Opposing effects of rheumatoid arthritis and low dose prednisolone on arginine metabolomics Atherosclerosis 2017 266 190 195 2-s2.0-85031090848 10.1016/j.atherosclerosis.2017.10.004 29035782

상세보기
26 Zabek A. Swierkot J. Malak A. Application of 1H NMR-based serum metabolomic studies for monitoring female patients with rheumatoid arthritis Journal of Pharmaceutical and Biomedical Analysis 2016 117 544 550 2-s2.0-84944463857 10.1016/j.jpba.2015.10.007 26476882

상세보기
27 Wang Q. Xu R. MetabolitePredict: A de novo human metabolomics prediction system and its applications in rheumatoid arthritis Journal of Biomedical Informatics 2017 71 222 228 2-s2.0-85020854069 10.1016/j.jbi.2017.06.002 28600026

상세보기
28 Patel S. Ahmed S. Emerging field of metabolomics: Big promise for cancer biomarker identification and drug discovery Journal of Pharmaceutical and Biomedical Analysis 2015 107 63 74 2-s2.0-84920733760 10.1016/j.jpba.2014.12.020 25569286

상세보기
29 Kang J. Zhu L. Lu J. Zhang X. Application of metabolomics in autoimmune diseases: Insight into biomarkers and pathology Journal of Neuroimmunology 2015 279 C 25 32 2-s2.0-84927171571 10.1016/j.jneuroim.2015.01.001 25669996

상세보기
30 Velenosi T. J. Hennop A. Feere D. A. Untargeted plasma and tissue metabolomics in rats with chronic kidney disease given AST-120 Scientific Reports 2016 6 2-s2.0-84959512909

상세보기
31 Wishart D. S. Emerging applications of metabolomics in drug discovery and precision medicine Nature Reviews Drug Discovery 2016 10.1038/nrd.2016.32

상세보기
32 Ried J. S. Baurecht H. Stückler F. Integrative genetic and metabolite profiling analysis suggests altered phosphatidylcholine metabolism in asthma Allergy: European Journal of Allergy and Clinical Immunology 2013 68 5 629 636 2-s2.0-84876678696 10.1111/all.12110 23452035

상세보기
33 Chang C. Guo Z.-G. He B. Yao W.-Z. Metabolic alterations in the sera of Chinese patients with mild persistent asthma: A GC-MS-based metabolomics analysis Acta Pharmacologica Sinica 2015 36 11 1356 1366 2-s2.0-84946542228 10.1038/aps.2015.102 26526201

상세보기
34 Mastrangelo A. Ferrarini A. Rey-Stolle F. García A. Barbas C. From sample treatment to biomarker discovery: A tutorial for untargeted metabolomics based on GC-(EI)-Q-MS Analytica Chimica Acta 2015 900 21 35 2-s2.0-84960445225 10.1016/j.aca.2015.10.001 26572836

상세보기
35 Pan H.-C. Kao T.-K. Ou Y.-C. Protective effect of docosahexaenoic acid against brain injury in ischemic rats The Journal of Nutritional Biochemistry 2009 20 9 715 725 2-s2.0-68249126664 10.1016/j.jnutbio.2008.06.014 18805685

상세보기
36 Pan P.-H. Lin S.-Y. Ou Y.-C. Stearic acid attenuates cholestasis-induced liver injury Biochemical and Biophysical Research Communications 2010 391 3 1537 1542 2-s2.0-73949084659 10.1016/j.bbrc.2009.12.119 20036638

상세보기
37 Nanji A. A. Jokelainen K. Tipoe G. L. Rahemtulla A. Dannenberg A. J. Dietary saturated fatty acids reverse inflammatory and fibrotic changes in rat liver despite continued ethanol administration The Journal of Pharmacology and Experimental Therapeutics 2001 299 2 638 644 2-s2.0-0034757922 11602676

상세보기
38 Nanji A. A. Zakim D. Rahemtulla A. Dietary saturated fatty acids down-regulate cyclooxygenase-2 and tumor necrosis factor alfa and reverse fibrosis in alcohol-induced liver disease in the rat Hepatology 1997 26 6 1538 1545 2-s2.0-0030665615 10.1002/hep.510260622 9397995

상세보기
39 Wang Z.-J. Liang C.-L. Li G.-M. Yu C.-Y. Yin M. Stearic acid protects primary cultured cortical neurons against oxidative stress Acta Pharmacologica Sinica 2007 28 3 315 326 2-s2.0-33847183532 10.1111/j.1745-7254.2007.00512.x 17302992

상세보기
40 Nishitani Y. Okazaki S. Imabayashi K. Saturated and monounsaturated fatty acids increase interleukin-10 production in rat hepatocytes. Nihon Arukōru Yakubutsu Igakkai zasshi = Japanese journal of alcohol studies & drug dependence 2007 42 1 32 35 2-s2.0-34250199370 17447468
41 Schaeffler A. Gross P. Buettner R. Fatty acid-induced induction of Toll-like receptor-4/nuclear factor- κ B pathway in adipocytes links nutritional signalling with innate immunity The Journal of Immunology 2009 126 2 233 245 2-s2.0-58149310977 10.1111/j.1365-2567.2008.02892.x 18624726

상세보기
42 Ho W. E. Xu Y.-J. Xu F. Anti-malarial drug artesunate restores metabolic changes in experimental allergic asthma Metabolomics 2015 11 2 380 390 2-s2.0-84944750031 10.1007/s11306-014-0699-x

상세보기
43 Xu W. Ghosh S. Comhair S. A. A. Increased mitochondrial arginine metabolism supports bioenergetics in asthma The Journal of Clinical Investigation 2016 126 7 2465 2481 2-s2.0-84978402760 10.1172/JCI82925 27214549

상세보기
44 Meurs H. McKay S. Maarsingh H. Increased arginase activity underlies allergen-induced deficiency of cNos-derived nitric oxide and airway hyperresponsiveness British Journal of Pharmacology 2002 136 3 391 398 2-s2.0-0036016110 10.1038/sj.bjp.0704725 12023942

상세보기
45 Lara A. Khatri S. B. Wang Z. Alterations of the arginine metabolome in asthma American Journal of Respiratory and Critical Care Medicine 2008 178 7 673 681 2-s2.0-52749084926 10.1164/rccm.200710-1542OC 18635886

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