[논문]Simultaneous Determination of Pyrethroid, Organophosphate and Carbamate Metabolites in Human Urine by Gas Chromatography-Mass Spectrometry (GCMS)

Hung, Chien-Che; Simaremare, Sailent Rizki Sari; Hsieh, Chia-Jung; Yiin, Lih-Ming

doi:10.3390/app9050879

Simultaneous Determination of Pyrethroid, Organophosphate and Carbamate Metabolites in Human Urine by Gas Chromatography-Mass Spectrometry (GCMS) 원문보기

Applied sciences, v.9 no.5, 2019년, pp.879 -

Hung, Chien-Che (Institute of Medical Sciences, Tzu Chi University, 701, Sec. 3, Zhongyang Road, Hualien City 97004, Taiwan) , Simaremare, Sailent Rizki Sari (Institute of Medical Sciences, Tzu Chi University, 701, Sec. 3, Zhongyang Road, Hualien City 97004, Taiwan) , Hsieh, Chia-Jung (Department of Public Health, Tzu Chi University, 701, Sec. 3, Zhongyang Road, Hualien City 97004, Taiwan) , Yiin, Lih-Ming (Department of Public Health, Tzu Chi University, 701, Sec. 3, Zhongyang Road, Hualien City 97004, Taiwan)

Abstract ▼ AI-Helper

We have developed a rapid, sensitive, and reliable method for simultaneous determination of the urinary metabolites of common insecticides in a single analytical run using gas chromatography-mass spectrometry (GCMS). Thirteen metabolites, one originating from carbamate, six from organophosphates, and seven from pyrethroids, were selected for method validation. Samples at different concentrations (0.5-15 µg/L) were prepared by mixing working solutions containing the analytes with blank urine. After acid hydrolysis for 45 min at 90 °C, samples were processed with liquid-liquid extraction and derivatization by N-tert-butyldimethylsilyl-N-methyltrifluoroacetamide (MTBSTFA) before analysis on GCMS. The limits of detection for all thirteen analytes were below 0.1 µg/L. The recovery rates, evaluated at two concentrations (1, 10 µg/L), were found to be 90.48%, on average. The precision of multiple analyses at three different concentrations (0.5, 5, 15 µg/L) within one day or between 10 days was evaluated, and the resultant relative standard deviations were 8.1% or under. We also applied this method to analyze genuine urine samples collected from 30 human subjects, and successfully detected all the metabolites, with detection frequencies more than 50% for pyrethroid metabolites. In summary, this method is not only as good as others in performance, but is advantageous in terms of cost effectiveness and multiplicity of analytes.

참고문헌 (29)

10.1039/b108601b Glynne-Jones (2001). Pyrethrum, The Royal Society of Chemistry.

상세보기
Yu Increased risk of attention-deficit/hyperactivity disorder associated with exposure to organophosphate pesticide in Taiwanese children Andrology 2016 10.1111/andr.12183 4 695

상세보기
Howard, P.H. (1989). Handbook of Environmental Fate and Exposure Data for Organic Chemicals, Lewis Publishers. [2nd ed.].
10.1016/B978-0-12-374367-1.00066-5 Timchalk, C. (2010). Organophosphorus Insecticide Pharmacokinetics. Hayes’ Handbook of Pesticide Toxicology (Third Edition), Academic Press. [2nd ed.]. Chapter 66.
Eyer Management of acute organophosphorus pesticide poisoning Lancet 2008 10.1016/S0140-6736(07)61202-1 371 597

상세보기
WHO (2017). Evaluation of Five Organophosphate Insecticides and Herbicides, World Health Organization. Iarc Monog Eval Carc.
IPCS (2009). The WHO Recommended Classification of Pesticides by Hazard and Guidelines to Classification, World Health Organization.
Lenga Biomarker of pyrethrum exposure Toxicol. Lett. 2006 10.1016/j.toxlet.2005.09.030 162 195

상세보기
(2018, November 06). Permethrin Facts (Reregistration Eligibility Decision (RED) Fact Sheet), Available online: https://archive.epa.gov/pesticides/reregistration/web/html/permethrin_fs.html.
Duarte Insecticide Reproductive Toxicity Profile: Organophosphate, Carbamate and Pyrethroids J. Toxins 2017 4 1
Predictors of exposure to organophosphate pesticides in schoolchildren in the Province of Talca, Chile Environ. Int. 2012 10.1016/j.envint.2012.06.002 47 28

상세보기
Oulhote Urinary metabolites of organophosphate and pyrethroid pesticides and behavioral problems in canadian children Environ. Health Perspect. 2013 10.1289/ehp.1306667 121 1378

상세보기
Petropoulou Gas chromatographic-tandem mass spectrometric method for the quantitation of carbofuran, carbaryl and their main metabolites in applicators’ urine J. Chromatogr. A 2006 10.1016/j.chroma.2005.12.058 1108 99

상세보기
Scollon In Vitro Metabolism of Pyrethroid Pesticides by Rat and Human Hepatic Microsomes and Cytochrome P450 Isoforms Drug Metab. Dispos. 2009 10.1124/dmd.108.022343 37 221

상세보기
Crow Hydrolysis of pyrethroids by human and rat tissues: Examination of intestinal, liver and serum carboxylesterases Toxicol. Appl. Pharm. 2007 10.1016/j.taap.2007.03.002 221 1

상세보기
Ahn Immunoassays and Biosensors for Monitoring Environmental and Human Exposure to Pyrethroid Insecticides J. Agric. Food Chem. 2011 10.1021/jf1033569 59 2792

상세보기
Olsson A Liquid Chromatography-Tandem Mass Spectrometry Multiresidue Method for Quantification of Specific Metabolites of Organophosphorus Pesticides, Synthetic Pyrethroids, Selected Herbicides, and DEET in Human Urine Anal. Chem. 2004 10.1021/ac0355404 76 2453

상세보기
Angerer Determination of metabolites of pyrethroids in human urine using solid-phase extraction and gas chromatography-mass spectrometry J. Chromatogr. B 1997 10.1016/S0378-4347(97)00174-6 695 217

상세보기
Bravo Quantitation of Dialkyl Phosphate Metabolites of Organophosphate Pesticides in Human Urine Using GC-MS-MS with Isotopic Internal Standards J. Anal. Toxicol. 2002 10.1093/jat/26.5.245 26 245

상세보기
Guo Simultaneous determination of eight metabolites of organophosphate and pyrethroid pesticides in urine J. Environ. Sci. Health B 2017 10.1080/03601234.2016.1224695 52 1

상세보기
Hung Pesticides in indoor and outdoor residential dust: A pilot study in a rural county of Taiwan Environ. Sci. Pollut. Res. 2018 10.1007/s11356-018-2413-4 23 23349

상세보기
Arrebola Monitoring of pyrethroid metabolites in human urine using solid-phase extraction followed by gas chromatography-tandem mass spectrometry Anal. Chim. Acta 1999 10.1016/S0003-2670(99)00519-X 401 45

상세보기
Needham Determination of Dialkyl Phosphate Metabolites of Organophosphorus Pesticides in Human Urine by Automated Solid-Phase Extraction, Derivatization, and Gas Chromatography-Mass Spectrometry J. Anal. Toxicol. 2008 10.1093/jat/32.9.721 32 721

상세보기
Schettgen New gas chromatographic-mass spectrometric method for the determination of urinary pyrethroid metabolites in environmental medicine J. Chromatogr. B 2002 10.1016/S0378-4347(01)00452-2 778 121

상세보기
Leng Simultaneous determination of pyrethroid and pyrethrin metabolites in human urine by gas chromatography-high resolution mass spectrometry J. Chromatogr. B 2005 10.1016/j.jchromb.2004.10.044 814 285

상세보기
Dulaurent Simultaneous determination of five synthetic pyrethroid metabolites in urine by liquid chromatography-tandem mass spectrometry: Application to 39 persons without known exposure to pyrethroids Toxicol. Lett. 2012 10.1016/j.toxlet.2011.08.016 210 248

상세보기
Sinha Analysis of dialkyl urine metabolites of organophosphate pesticides by a liquid chromatography mass spectrometry technique Anal. Methods 2014 10.1039/C3AY41958D 6 1825

상세보기
INSQ (2009). Analytical Method for the Determination of Pyrethroid Metabolites in Urine by GC-MS, Condensed Version for CHMS, Analytical Chemistry for Environmental Sciences, Quebec, Canada, 08.25.2009, Institut national de santé publique.
Davis Semi-automated solid phase extraction method for the mass spectrometric quantification of 12 specific metabolites of organophosphorus pesticides, synthetic pyrethroids, and select herbicides in human urine J. Chromatogr. B 2013 10.1016/j.jchromb.2013.04.005 929 18

상세보기

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Simultaneous Determination of Pyrethroid, Organophosphate and Carbamate Metabolites in Human Urine by Gas Chromatography-Mass Spectrometry (GCMS) 원문보기

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