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NTIS 바로가기Zeitschrift für Naturforschung. C, A journal of biosciences, v.74 no.7/8, 2019년, pp.167 - 174
Kim, Mihyang (Department of Food and Nutrition, College of Medical and Life Science, Silla University, Busan 46958, Republic of Korea) , Kang, Jeong Hyeon (Department of Food and Nutrition, College of Medical and Life Science, Silla University, Busan 46958, Republic of Korea) , Oh, Geun Hye (Department of Food and Nutrition, College of Medical and Life Science, Silla University, Busan 46958, Republic of Korea) , Park, Mi Hwa
AbstractOsteoporosis is one of the most common bone diseases, occurring due to an imbalance between bone formation and bone resorption. The aim of this study was to investigate the effects ofIshige sinicola, a brown alga, on osteoblast differentiation through the activation of the bone morphogenetic protein 2 (BMP-2)/runt-related transcription factor 2 (Runx2) signalling pathway in MC3T3-E1 cells. A cell proliferation assay, alkaline phosphatase (ALP) activity assay, alizarin red staining, and expression analysis of osteoblastic genes were carried out to assess MC3T3-E1 cell proliferation and osteoblastic differentiation. We found thatI. sinicolaextract (ISE) increased cell proliferation in a dose-dependent manner.Ishige sinicolaextract markedly promoted ALP activity and mineralization. Alizarin red S staining demonstrated that ISE treatment tended to increase extracellular matrix calcium accumulation. Moreover, ISE up-regulated the osteoprotegerin/receptor activator of nuclear factor κB ligand ratio.Ishige sinicolaextract also increased the protein expression levels of type 1 collagen, ALP, osteocalcin, osterix, BMP-2, and Runx2. Therefore, ISE showed potential in stimulating osteoblastic bone formation, and it might be useful for the prevention and treatment of osteoporosis.
10.1126/science.289.5484.1508 Rodan GA, Martin TJ. Therapeutic approaches to bone diseases. Science 2000;289:1508-14.1096878110.1126/science.289.5484.1508
10.1248/jhs.48.209 Yamaguchi M. Isoflavone and bone metabolism: its cellular mechanism and preventive role in bone loss. J Health Sci 2002;48:209-22.10.1248/jhs.48.209
10.1126/science.289.5484.1501 Ducy P, Schinke T, Karsenty G. The osteoblast: a sophisticated fibroblast under central surveillance. Science 2000;289:1501-4.1096877910.1126/science.289.5484.1501
10.1056/NEJM199502023320506 Manolagas SC, Jilka RL. Bone marrow, cytokines and bone remodeling. Emerging insights into the pathophysiology of osteoporosis. N Engl J Med 1995;332:305-311.781606710.1056/NEJM199502023320506
10.1016/j.ejphar.2010.10.048 Yonezawa T, Hasequwa S, Asai M, Ninomiya T, Sasaki T, Cha BY, et al. Harmine, a β-carboline alkaloid, inhibits osteoclast differentiation and bone resorption in vitro and in vivo. Eur J Pharmacol 2011;650:511-8.10.1016/j.ejphar.2010.10.04821047508
10.1210/edrv.21.4.0403 Yamaguchi A, Komori T, Suda T. Regulation of osteoblast differentiation mediated by bone morphogenetic proteins, hedgehogs, and Cbfa1. Endocr Rev 2000;21:393-411.10.1210/edrv.21.4.040310950158
10.1016/S0070-2153(06)78004-8 Garrett IR. Anabolic agents and the bone morphogenetic protein pathway. Curr Top Dev Biol 2007;78:127-71.1733891610.1016/S0070-2153(06)78004-8
10.1083/jcb.127.6.1755 Katagiri T, Yamaguchi A, Komaki M, Abe E, Takahashi N, Ikeda T, et al. Bone morphogenetic protein-2 converts the differentiation pathway of C2C12 myoblasts into the osteoblast lineage. J Cell Biol 1994;127:1755-66.7798324
10.1083/jcb.113.3.681 Yamaguchi A, Katagiri T, Ikeda T, Wozney JM, Rosen V, Wang EA, et al. Recombinant human bone morphogenetic protein-2 stimulates osteoblastic maturation and inhibits myogenic differentiation in vitro. J Cell Biol 1991;113:681-7.10.1083/jcb.113.3.6811849907
10.2106/00004623-200307000-00010 Meyer RA Jr, Meyer MH, Tenholder M, Wondracek S, Wasseman R, Garges P. Gene expression in older rats with delayed union of femoral fractures. J Bone Joint Surg Am 2003;85:1243-54.10.2106/00004623-200307000-0001012851349
10.1016/j.foodchem.2007.08.081 Chandini SK, Ganesan P, Bhaskar N. In vitro antioxidant activities of three selected brown seaweeds of India. Food Chem 2008;107:707-13.10.1016/j.foodchem.2007.08.081
10.1016/j.jep.2007.10.032 Kang JY, Khan MN, Park NH, Cho JY, Lee MC, Fujii H, et al. Antipyretic, analgesic, and anti-inflammatory activities of the seaweed Sargassum fulvellum and Sargassum thunbergii in mice. J Ethnopharmacol 2008;116:187-90.1807907710.1016/j.jep.2007.10.032
10.1016/j.fct.2011.12.032 Lee SH, Min KH, Han JS, Lee DH, Park DB, Jung WK, et al. Effects of brown alga, Ecklonia cava on glucose and lipid metabolism in C57BL/KsJ-db/db mice, a model of type 2 diabetes mellitus. Food Chem Toxicol 2012;50:575-82.10.1016/j.fct.2011.12.03222227338
Choi JS, Bae HJ, Kim SJ, Choi IS. In vitro antibacterial and anti-inflammatory properties of seaweed extracts against acne inducing bacteria, Propionibacterium acnes. J Environ Biol 2011;32:313-8.22167943
10.3390/md11061783 Kang JI, Kim EJ, Kim MK, Jeon YJ, Kang SM, Koh YS, et al. The promotion effect of Ishige sinicola on hair growth. Mar Drugs 2013;11:1783-99.10.3390/md11061783
10.1093/clinchem/29.5.751 Tietz NW, Burtis CA, Duncan P, Ervin K, Petitclerc CJ, Rinker AD, et al. A reference method for measurement of alkaline phosphatase activity in human serum. Clin Chem 1983;29:751-61.6404566
10.1016/j.clinbiochem.2008.02.011 Pilichou A, Papassotiriou I, Michalakakou K, Fessatou S, Fandridis E, Papachristou G, et al. High levels of synovial fluid osteoprotegerin (OPG) and increased serum ratio of receptor activator of nuclear factor-kappa B ligand (RANKL) to OPG correlate with disease severity in patients with primary knee osteoarthritis. Clin Biochem 2008;41:746-9.1835545310.1016/j.clinbiochem.2008.02.011
10.1007/s00280-006-0300-z Wang W, Zhao Y, Rayburn ER, Hill DL, Wang H, Zhang R. In vitro anti-cancer activity and structure-activity relationships of natural products isolated from fruits of Panax ginseng. Cancer Chemother Pharmacol 2007;59:589-601.10.1007/s00280-006-0300-z16924497
10.1038/nrd916 Goltzman D. Discoveries, drugs and skeletal disorders. Nat Rev Drug Discov 2002;1:784-96.1236025610.1038/nrd916
10.1016/j.phymed.2009.08.007 Hsieh TP, Sheu SY, Sun JS, Chen MH, Liu MH. Icariin isolated from Epimedium pubescens regulates osteoblasts anabolism through BMP-2 SMAD4, and Cbfa 1 expression. Phytomedicine 2010;17:414-23.10.1016/j.phymed.2009.08.007
10.1016/j.bbrc.2003.12.073 Bai XC, Lu D, Bai J, Zheng H, Ke ZY, Li XM, et al. Oxidative stress inhibits osteoblastic differentiation of bone cells by ERK and NF-kappaB. Biochem Biophys Res Commun 2004;314:197-207.10.1016/j.bbrc.2003.12.07314715266
10.1002/jor.21147 Serigano K, Sakai D, Hiyama A, Tamura F, Tanaka M, Mochida J. Effect of cell number on mesenchymal stem cell transplantation in a canine disc degeneration model. J Orthop Res 2010;28:1267-75.10.1002/jor.21147
10.1007/s11010-008-9788-3 Orimo H, Shimada T. The role of tissue-nonspecific alkaline phosphatase in the phosphate-induced activation of alkaline phosphatase and mineralization in SaOS-2 human osteoblast-like cells. Mol Cell Biochem 2008;315:51-60.1850065710.1007/s11010-008-9788-3
10.1359/jbmr.2000.15.10.1879 Wennberg C, Hessle L, Jundberg P, Mauro S, Narisawa S, Lerner UH, et al. Functional characterization of osteoblasts and osteoclasts from alkaline phosphatase knockout mice. J Bone Miner Res 2000;15:1879-88.10.1359/jbmr.2000.15.10.187911028439
10.1016/j.biomaterials.2005.01.006 Maeno S, Niki Y, Matsumoto H, Morioka H, Yatabe T, Funayama A, et al. The effect of calcium ion concentration on osteoblast viability, proliferation, and differentiation in monolayer and 3D culture. Biomaterials 2005;26:4847-55.10.1016/j.biomaterials.2005.01.00615763264
10.2302/kjm.52.1 Miyamoto T, Suda T. Differentiation and function of osteoclasts. Keio J Med 2003;52:1-7.1271301610.2302/kjm.52.1
10.1111/j.1600-079X.2011.00875.x Park KH, Kang JW, Lee EM, Kim JS, Rhee YH, Kim M, et al. Melatonin promotes osteoblastic differentiation through the BMP/ERK/Wnt signaling pathways. J Pineal Res 2011;51: 187-94.10.1111/j.1600-079X.2011.00875.x21470302
10.1089/ten.2006.12.3459 Sumanasinghe RD, Bernacki SH, Loboa EG. Osteogenic differentiation of human mesenchymal stem cells in collagen matrices: effect of uniaxial cyclic tensile strain on bone morphogenetic protein (BMP-2) mRNA expression. Tissue Eng 2006;12:3459-65.10.1089/ten.2006.12.3459
10.1615/CritRevEukaryotGeneExpr.v14.i12.10 Lian JB, Javed A, Zaidi SK, Lengner C, Montecino M, van Wijnen AJ, et al. Regulatory controls of osteoblast growth and differentiation: role of Runx/Cbfa/AMI factors. Crit Rev Eukaryot Gene Expr 2004;14:1-41.10.1615/CritRevEukaryotGeneExpr.v14.i12.10
10.1016/S0092-8674(00)80257-3 Ducy P, Zhang R, Geoffroy V, Ridall AL, Karsenty G. Osf2/Cbfa1: a transcriptional activator of osteoblast differentiation. Cell 1997;89:747-54.10.1016/S0092-8674(00)80257-39182762
10.1016/S0092-8674(01)00622-5 Nakashima K, Zhou X, Kunkel G, Zhang Z, Deng JM, Behringer RR, et al. The novel zinc finger-containing transcription factor osterix is required for osteoblast differentiation and bone formation. Cell 2002;108:17-29.10.1016/S0092-8674(01)00622-511792318
10.1177/10454411920030030501 Lian J, Stein G. Concepts of osteoblast differentiation: basis for modulation of bone cell development and tissue formation. Crit Rev Oral Biol Med 1992;3:269-305.10.1177/104544119200300305011571474
10.3892/mmr.2016.6062 Jin X, Zhu L, Li X, Jia J, Zhang Y, Sun X, et al. Low-molecular weight fucoidan inhibits the differentiation of osteoclasts and reduces osteoporosis in ovariectomized rats. Mol Med Rep 2017;15:890-8.10.3892/mmr.2016.6062
10.1016/j.foodchem.2012.11.026 Balboa EM, Conde E, Moure A, Falque E, Dominguez H. In vitro antioxidant properties of crude extracts and compounds from brown algae. Food Chem 2013;138: 1764-85.2341130910.1016/j.foodchem.2012.11.026
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