[논문]Ishige sinicola extract stimulates osteoblast proliferation and differentiation via the bone morphogenetic protein 2/runt-related gene 2 signalling pathway

Kim, Mihyang; Kang, Jeong Hyeon; Oh, Geun Hye; Park, Mi Hwa

doi:10.1515/znc-2018-0044

Ishige sinicola extract stimulates osteoblast proliferation and differentiation via the bone morphogenetic protein 2/runt-related gene 2 signalling pathway

Zeitschrift für Naturforschung. C, A journal of biosciences, v.74 no.7/8, 2019년, pp.167 - 174

Kim, Mihyang (Department of Food and Nutrition, College of Medical and Life Science, Silla University, Busan 46958, Republic of Korea) , Kang, Jeong Hyeon (Department of Food and Nutrition, College of Medical and Life Science, Silla University, Busan 46958, Republic of Korea) , Oh, Geun Hye (Department of Food and Nutrition, College of Medical and Life Science, Silla University, Busan 46958, Republic of Korea) , Park, Mi Hwa

Abstract

AbstractOsteoporosis is one of the most common bone diseases, occurring due to an imbalance between bone formation and bone resorption. The aim of this study was to investigate the effects ofIshige sinicola, a brown alga, on osteoblast differentiation through the activation of the bone morphogenetic protein 2 (BMP-2)/runt-related transcription factor 2 (Runx2) signalling pathway in MC3T3-E1 cells. A cell proliferation assay, alkaline phosphatase (ALP) activity assay, alizarin red staining, and expression analysis of osteoblastic genes were carried out to assess MC3T3-E1 cell proliferation and osteoblastic differentiation. We found thatI. sinicolaextract (ISE) increased cell proliferation in a dose-dependent manner.Ishige sinicolaextract markedly promoted ALP activity and mineralization. Alizarin red S staining demonstrated that ISE treatment tended to increase extracellular matrix calcium accumulation. Moreover, ISE up-regulated the osteoprotegerin/receptor activator of nuclear factor κB ligand ratio.Ishige sinicolaextract also increased the protein expression levels of type 1 collagen, ALP, osteocalcin, osterix, BMP-2, and Runx2. Therefore, ISE showed potential in stimulating osteoblastic bone formation, and it might be useful for the prevention and treatment of osteoporosis.

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참고문헌 (39)

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Ishige sinicola extract stimulates osteoblast proliferation and differentiation via the bone morphogenetic protein 2/runt-related gene 2 signalling pathway

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Ishige sinicola extract stimulates osteoblast proliferation and differentiation via the bone morphogenetic protein 2/runt-related gene 2 signalling pathway

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