[논문]A new approach for routine quantification of microplastics using Nile Red and automated software (MP-VAT)

Prata, Joana C.; Reis, Vanessa; Matos, Joã; o T.V.; da Costa, Joã; o P.; Duarte, Armando C.; Rocha-Santos, Teresa

doi:10.1016/j.scitotenv.2019.07.060

[해외논문] A new approach for routine quantification of microplastics using Nile Red and automated software (MP-VAT)

The Science of the total environment, v.690, 2019년, pp.1277 - 1283

Prata, Joana C. (Corresponding author.) , Reis, Vanessa , Matos, João T.V. , da Costa, João P. , Duarte, Armando C. , Rocha-Santos, Teresa

Abstract ▼ AI-Helper

Abstract Microplastics are widespread contaminants in the environment. However, most identification protocols rely on long and subjective visual counting, which could be improved using staining dyes. Thus, the objective of this work is to identify the best staining dye protocol and create an objective and quick automated counting software for microplastics. Tests were conducted to identify the most appropriate of eight staining dye solutions and of six wavelengths for virgin and weathered synthetic polymers, textile fibers, natural organic matter and filters. Nile Red produced the best results (without interfering in infrared spectra) rendering microplastics fluorescent at 254 nm, but with limited number of fluorescent polymers, and at 470 nm (with orange filter), with fluorescence of plastics as well as natural organic matter (requiring a digestion step). Next, a script was developed in ImageJ for the automatic quantification and characterization in shape (fiber, fragment, particle) and size of fluorescent microplastics, the Microplastics Visual Analysis Tool (MP-VAT). MP-VAT was evaluated, producing recovery rates in the range of 89.0–111.1% in spiked filters under 470 nm. Furthermore, this package is accompanied by a script that sets a scale from a known filter diameter, MP-SCALE, and a script that allows user threshold setting, MP-ACT. Highlights Nile Red was the best of eight dyes for staining microplastics. Wavelengths of 254 and 470 nm produce the best results for Nile Red. MP-VAT was developed for automatic quantification of fluorescent microplastics. MP-VAT quantifies and characterizes microplastics based on shape and size. Recovery rates of 89.0 to 111.1% deem MP-VAT accurate for microplastic quantification. Graphical abstract [DISPLAY OMISSION]

Keyword

참고문헌 (23)

Polym. Int. Christie 34 351 1994 10.1002/pi.1994.210340401 Pigments, dyes and fluorescent brightening agents for plastics: an overview

상세보기
J. Environ. Sci. Health A Da Costa 53 9 866 2018 10.1080/10934529.2018.1455381 Degradation of polyethyelene microplastics in seawater: insights into the environmental degradation of polymers

상세보기
Mar. Pollut. Bull. Davidson 62 1821 2012 10.1016/j.marpolbul.2012.06.005 Boring crustaceans damage polystyrene floats under docks polluting marine waters with microplastics

상세보기
Sci. Total Environ. Dris 618 157 2018 10.1016/j.scitotenv.2017.11.009 Synthetic and non-synthetic anthropogenic fibers in a river under the impact of Paris Megacity: sampling methodological aspects and flux estimations

상세보기
Environ. Sci. Technol. Erni-Cassola 51 23 13641 2017 10.1021/acs.est.7b04512 Lost, but found with Nile red; a novel method to detect and quantify small microplastics (20 um-1mm) in environmental samples

상세보기
PLoS One Forero 4 5 2009 10.1371/journal.pone.0005441 DeadEasy caspase: automatic counting of apoptotic cells in drosophila

상세보기
Anal. Biochem. Grishagin 473 63 2015 10.1016/j.ab.2014.12.007 Automatic cell counting with ImageJ

상세보기
Mol. Cell. Toxicol. Ha 14 4 353 2018 10.1007/s13273-018-0039-8 The environmental effects of microplastics on aquatic ecosystems

상세보기
Mar. Pollut. Bull. Isobe 89 1-2 324 2014 10.1016/j.marpolbul.2014.09.041 Selective transporto f microplastics and mesoplastics by drifting in coastal waters

상세보기
Mar. Pollut. Bull. Isobe 114 1 623 2017 10.1016/j.marpolbul.2016.09.037 Microplastics in the Southern Ocean

상세보기
PLoS One Kosuth 13 4 2018 10.1371/journal.pone.0194970 Anthropogenic contamination of tap water, beer, and sea salt

상세보기
Food Addit. Contam. Part A Liebezeit 31 9 2014 10.1080/19440049.2014.945099 Synthetic particles as contaminants in German bears

상세보기
Sci. Rep. Maes 7 2017 10.1038/srep44501 A rapid-screening approach to detect and quantify microplastics based on fluorescent tagging with Nile Red

상세보기
Front. Mar. Sci. Maes 4 136 2017 10.3389/fmars.2017.00135 Microplastic baseline surveys at the water surface and in sediments of the North-East Atlantic

상세보기
J. Nanopart. Res. Mahedevan 15 1477 2013 10.1007/s11051-013-1477-9 Diffusion transport of nanoparticles at nanochannel boundaries

상세보기
Front. Chem. Mason 6 407 2018 10.3389/fchem.2018.00407 Synthetic polymer contamination in bottled water

상세보기
Sci. Rep. McQuaid 6 2016 10.1038/srep19442 Imaging and radiation effects of gold nanoparticles in tumor cells

상세보기
Trends Anal. Chem. Prata 110 150 2019 10.1016/j.trac.2018.10.029 Methods for sampling and detection of microplastics in water and sediment: a critical review

상세보기
Sci. Total Environ. Prata 686 131 2019 10.1016/j.scitotenv.2019.05.456 Identifying a quick and efficient method of removing organic matter without damaging microplastic samples

상세보기
Mar. Pollut. Bull. Shim 113 469 2016 10.1016/j.marpolbul.2016.10.049 Identification and quantification of microplastics using Nile Red staining

상세보기
SDRP J. Earth Sci. Environ. Stud. Tamminga 2 2 2017 Nile Red staining as a subsidiary method for microplastic quantification: a comparison of three solvents and factors influencing application reliability
Textile World
Water Res. Ziajahromi 112 93 2017 10.1016/j.watres.2017.01.042 Wastewater treatment plants as a pathway for microplastics: development of a new approach to sample wastewater-based microplastics

상세보기

활용도 분석정보

상세보기

다운로드

내보내기

활용도 Top5 논문

해당 논문의 주제분야에서 활용도가 높은 상위 5개 콘텐츠를 보여줍니다.
더보기 버튼을 클릭하시면 더 많은 관련자료를 살펴볼 수 있습니다.

내보내기 구분	파일저장 인쇄 메일전송
구성항목	기본정보 상세정보 관리번호, 논문명, 저널/프로시딩명, 저자 , 발행년, 권, 호, 시작페이지, 끝페이지, 발행기관 관리번호, 논문명, 대등논문명, 저자 , 저널/프로시딩명, 발행기관, 발행년, 발행언어, 권, 호, 시작페이지, 끝페이지, ISBN, ISSN, 주제분야, 키워드, 초록(한글), 초록(영문), 저자(소속기관)
저장형식	Text(ASCII format) Excel format RefWorks Direct Export RIS format (for Reference Manager, ProCite, EndNote), Scholar's Aids, Mendeley
메일정보	받는사람 (필수) @ 보내는사람 (선택) @ 제목 내용 KISTI 검색결과 이메일 서비스
안내	총 건의 자료가 검색되었습니다. 다운받으실 자료의 인덱스를 입력하세요. (1-10,000) 검색결과의 순서대로 최대 10,000건 까지 다운로드가 가능합니다. 데이타가 많을 경우 속도가 느려질 수 있습니다.(최대 2~3분 소요) 다운로드 파일은 UTF-8 형태로 저장됩니다. 파일의 내용이 제대로 보이지 않을실 때는 웹브라우저 상단의 보기 -> 인코딩 -> 자동선택 여부를 확인하십시오. ~ Text(ASCII format) Excel format

연합인증

[해외논문] A new approach for routine quantification of microplastics using Nile Red and automated software (MP-VAT)

Abstract ▼ AI-Helper

Keyword

참고문헌 (23)

이 논문을 인용한 문헌

활용도 분석정보

활용도 Top5 논문

관련 콘텐츠

원문 URL 링크

이 논문과 함께 이용한 콘텐츠

AI-Helper ※ AI-Helper는 오픈소스 모델을 사용합니다.

선택된 텍스트

연합인증

[해외논문] A new approach for routine quantification of microplastics using Nile Red and automated software (MP-VAT)

Abstract ▼ AI-Helper

Keyword

참고문헌 (23)

이 논문을 인용한 문헌

활용도 분석정보

활용도 Top5 논문 더보기

관련 콘텐츠

원문 URL 링크

이 논문과 함께 이용한 콘텐츠

AI-Helper ※ AI-Helper는 오픈소스 모델을 사용합니다.

선택된 텍스트

활용도 Top5 논문