최소 단어 이상 선택하여야 합니다.
최대 10 단어까지만 선택 가능합니다.
다음과 같은 기능을 한번의 로그인으로 사용 할 수 있습니다.
NTIS 바로가기Microbial cell factories, v.11, 2012년, pp.97 - 97
Klatt, Stephan (Max Planck Institute for Molecular Genetics, Ihnestraße 63-73, 14195, Berlin, Germany) , Konthur, Zoltán (Max Planck Institute for Molecular Genetics, Ihnestraße 63-73, 14195, Berlin, Germany)
BackgroundSecretory signal peptides (SPs) are well-known sequence motifs targeting proteins for translocation across the endoplasmic reticulum membrane. After passing through the secretory pathway, most proteins are secreted to the environment. Here, we describe the modification of an expression vec...
Basak A Shervani NJ Mbikay M Kolajova M Recombinant proprotein convertase 4 (PC4) from Leishmania tarentolae expression system: purification, biochemical study and inhibitor design Protein Expr Purif 2008 60 2 117 126 10.1016/j.pep.2008.03.013 18485734
Breton M Zhao C Ouellette M Tremblay MJ Papadopoulou B A recombinant non-pathogenic Leishmania vaccine expressing human immunodeficiency virus 1 (HIV-1) Gag elicits cell-mediated immunity in mice and decreases HIV-1 replication in human tonsillar tissue following exposure to HIV-1 infection J Gen Virol 2007 88 Pt 1 217 225 17170454
Yakovich AJ Ragone FL Alfonzo JD Sackett DL Werbovetz KA Leishmania tarentolae: purification and characterization of tubulin and its suitability for antileishmanial drug screening Exp Parasitol 2006 114 4 289 296 10.1016/j.exppara.2006.04.008 16753146
Breitling R Klingner S Callewaert N Pietrucha R Geyer A Ehrlich G Hartung R Muller A Contreras R Beverley SM Non-pathogenic trypanosomatid protozoa as a platform for protein research and production Protein Expr Purif 2002 25 2 209 218 10.1016/S1046-5928(02)00001-3 12135552
Fritsche C Sitz M Weiland N Breitling R Pohl HD Characterization of the growth behavior of Leishmania tarentolae: a new expression system for recombinant proteins J Basic Microbiol 2007 47 5 384 393 10.1002/jobm.200710111 17910102
Fritsche C Sitz M Wolf M Pohl HD Development of a defined medium for heterologous expression in Leishmania tarentolae J Basic Microbiol 2008 48 6 488 495 10.1002/jobm.200700389 18759244
Kushnir S Gase K Breitling R Alexandrov K Development of an inducible protein expression system based on the protozoan host Leishmania tarentolae Protein Expr Purif 2005 42 1 37 46 10.1016/j.pep.2005.03.004 15939291
Mureev S Kushnir S Kolesnikov AA Breitling R Alexandrov K Construction and analysis of Leishmania tarentolae transgenic strains free of selection markers Mol Biochem Parasitol 2007 155 2 71 83 10.1016/j.molbiopara.2007.05.007 17658188
Bird RE Hardman KD Jacobson JW Johnson S Kaufman BM Lee SM Lee T Pope SH Riordan GS Whitlow M Single-chain antigen-binding proteins Science 1988 242 4877 423 426 10.1126/science.3140379 3140379
Rapoport TA Transport of proteins across the endoplasmic reticulum membrane Science 1992 258 5084 931 936 10.1126/science.1332192 1332192
von Heijne G The signal peptide J Membr Biol 1990 115 3 195 201 10.1007/BF01868635 2197415
von Heijne G Life and death of a signal peptide Nature 1998 396 6707 111 113 10.1038/24036 9823886
Emanuelsson O Brunak S von Heijne G Nielsen H Locating proteins in the cell using TargetP, SignalP and related tools Nat Protoc 2007 2 4 953 971 10.1038/nprot.2007.131 17446895
Sakaguchi M Eukaryotic protein secretion Curr Opin Biotechnol 1997 8 5 595 601 10.1016/S0958-1669(97)80035-3 9353225
Borrero J Jimenez JJ Gutiez L Herranz C Cintas LM Hernandez PE Protein expression vector and secretion signal peptide optimization to drive the production, secretion, and functional expression of the bacteriocin enterocin A in lactic acid bacteria J Biotechnol 2011 156 1 76 86 10.1016/j.jbiotec.2011.07.038 21839785
Jonet MA Mahadi NM Murad AM Rabu A Bakar FD Rahim RA Low KO Illias RM Optimization of a heterologous signal peptide by site-directed mutagenesis for improved secretion of recombinant proteins in Escherichia coli J Mol Microbiol Biotechnol 2012 22 1 48 58 10.1159/000336524 22456489
Liu FC Chen HL Chong KY Hsu AL Chen CM Production of recombinant porcine colipase secreted by Pichia pastoris and its application to improve dietary fat digestion and growth of postweaning piglets Biotechnol Prog 2008 24 6 1333 1341 10.1002/btpr.46 19194948
Rakestraw JA Sazinsky SL Piatesi A Antipov E Wittrup KD Directed evolution of a secretory leader for the improved expression of heterologous proteins and full-length antibodies in Saccharomyces cerevisiae Biotechnol Bioeng 2009 103 6 1192 1201 10.1002/bit.22338 19459139
Futatsumori-Sugai M Tsumoto K Signal peptide design for improving recombinant protein secretion in the baculovirus expression vector system Biochem Biophys Res Commun 2009 391 1 931 935 19962965
de Wildt RM Mundy CR Gorick BD Tomlinson IM Antibody arrays for high-throughput screening of antibody-antigen interactions Nat Biotechnol 2000 18 9 989 994 10.1038/79494 10973222
Nielsen H Engelbrecht J Brunak S von Heijne G A neural network method for identification of prokaryotic and eukaryotic signal peptides and prediction of their cleavage sites Int J Neural Syst 1997 8 5–6 581 599 10065837
Wiese M Ilg T Lottspeich F Overath P Ser/Thr-rich repetitive motifs as targets for phosphoglycan modifications in Leishmania mexicana secreted acid phosphatase EMBO J 1995 14 6 1067 1074 7720697
Dortay H Schmockel SM Fettke J Mueller-Roeber B Expression of human c-reactive protein in different systems and its purification from Leishmania tarentolae Protein Expr Purif 2011 78 1 55 60 10.1016/j.pep.2011.03.010 21440634
Sugino M Niimi T Expression of multisubunit proteins in Leishmania tarentolae Methods Mol Biol 2012 824 317 325 10.1007/978-1-61779-433-9_16 22160906
Choo KH Tan TW Ranganathan S A comprehensive assessment of N-terminal signal peptides prediction methods BMC Bioinforma 2009 10 Suppl 15 S2 10.1186/1471-2105-10-S15-S2
Petersen TN Brunak S von Heijne G Nielsen H SignalP 4.0: discriminating signal peptides from transmembrane regions Nat Methods 2011 8 10) 785 786 21959131
Jacquemin MG Desqueper BG Benhida A Vander Elst L Hoylaerts MF Bakkus M Thielemans K Arnout J Peerlinck K Gilles JG Mechanism and kinetics of factor VIII inactivation: study with an IgG4 monoclonal antibody derived from a hemophilia A patient with inhibitor Blood 1998 92 2 496 506 9657749
Bendtsen JD Nielsen H von Heijne G Brunak S Improved prediction of signal peptides: SignalP 3.0 J Mol Biol 2004 340 4 783 795 10.1016/j.jmb.2004.05.028 15223320
Lei SP Lin HC Wang SS Callaway J Wilcox G Characterization of the Erwinia carotovora pelB gene and its product pectate lyase J Bacteriol 1987 169 9 4379 4383 3040692
von Heijne G Patterns of amino acids near signal-sequence cleavage sites Eur J Biochem 1983 133 1 17 21 10.1111/j.1432-1033.1983.tb07424.x 6852022
von Heijne G Analysis of the distribution of charged residues in the N-terminal region of signal sequences: implications for protein export in prokaryotic and eukaryotic cells EMBO J 1984 3 10 2315 2318 6499832
von Heijne G How signal sequences maintain cleavage specificity J Mol Biol 1984 173 2 243 251 10.1016/0022-2836(84)90192-X 6423828
Edman P A method for the determination of amino acid sequence in peptides Arch Biochem 1949 22 3 475 18134557
*원문 PDF 파일 및 링크정보가 존재하지 않을 경우 KISTI DDS 시스템에서 제공하는 원문복사서비스를 사용할 수 있습니다.
오픈액세스 학술지에 출판된 논문
※ AI-Helper는 부적절한 답변을 할 수 있습니다.