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Secretory signal peptide modification for optimized antibody-fragment expression-secretion in Leishmania tarentolae 원문보기

Microbial cell factories, v.11, 2012년, pp.97 - 97  

Klatt, Stephan (Max Planck Institute for Molecular Genetics, Ihnestraße 63-73, 14195, Berlin, Germany) ,  Konthur, Zoltán (Max Planck Institute for Molecular Genetics, Ihnestraße 63-73, 14195, Berlin, Germany)

Abstract AI-Helper 아이콘AI-Helper

BackgroundSecretory signal peptides (SPs) are well-known sequence motifs targeting proteins for translocation across the endoplasmic reticulum membrane. After passing through the secretory pathway, most proteins are secreted to the environment. Here, we describe the modification of an expression vec...

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참고문헌 (34)

  1. Basak A Shervani NJ Mbikay M Kolajova M Recombinant proprotein convertase 4 (PC4) from Leishmania tarentolae expression system: purification, biochemical study and inhibitor design Protein Expr Purif 2008 60 2 117 126 10.1016/j.pep.2008.03.013 18485734 

  2. Breton M Zhao C Ouellette M Tremblay MJ Papadopoulou B A recombinant non-pathogenic Leishmania vaccine expressing human immunodeficiency virus 1 (HIV-1) Gag elicits cell-mediated immunity in mice and decreases HIV-1 replication in human tonsillar tissue following exposure to HIV-1 infection J Gen Virol 2007 88 Pt 1 217 225 17170454 

  3. Yakovich AJ Ragone FL Alfonzo JD Sackett DL Werbovetz KA Leishmania tarentolae: purification and characterization of tubulin and its suitability for antileishmanial drug screening Exp Parasitol 2006 114 4 289 296 10.1016/j.exppara.2006.04.008 16753146 

  4. Breitling R Klingner S Callewaert N Pietrucha R Geyer A Ehrlich G Hartung R Muller A Contreras R Beverley SM Non-pathogenic trypanosomatid protozoa as a platform for protein research and production Protein Expr Purif 2002 25 2 209 218 10.1016/S1046-5928(02)00001-3 12135552 

  5. Fritsche C Sitz M Weiland N Breitling R Pohl HD Characterization of the growth behavior of Leishmania tarentolae: a new expression system for recombinant proteins J Basic Microbiol 2007 47 5 384 393 10.1002/jobm.200710111 17910102 

  6. Fritsche C Sitz M Wolf M Pohl HD Development of a defined medium for heterologous expression in Leishmania tarentolae J Basic Microbiol 2008 48 6 488 495 10.1002/jobm.200700389 18759244 

  7. Kushnir S Gase K Breitling R Alexandrov K Development of an inducible protein expression system based on the protozoan host Leishmania tarentolae Protein Expr Purif 2005 42 1 37 46 10.1016/j.pep.2005.03.004 15939291 

  8. Mureev S Kushnir S Kolesnikov AA Breitling R Alexandrov K Construction and analysis of Leishmania tarentolae transgenic strains free of selection markers Mol Biochem Parasitol 2007 155 2 71 83 10.1016/j.molbiopara.2007.05.007 17658188 

  9. Bird RE Hardman KD Jacobson JW Johnson S Kaufman BM Lee SM Lee T Pope SH Riordan GS Whitlow M Single-chain antigen-binding proteins Science 1988 242 4877 423 426 10.1126/science.3140379 3140379 

  10. Rapoport TA Transport of proteins across the endoplasmic reticulum membrane Science 1992 258 5084 931 936 10.1126/science.1332192 1332192 

  11. von Heijne G The signal peptide J Membr Biol 1990 115 3 195 201 10.1007/BF01868635 2197415 

  12. von Heijne G Life and death of a signal peptide Nature 1998 396 6707 111 113 10.1038/24036 9823886 

  13. Emanuelsson O Brunak S von Heijne G Nielsen H Locating proteins in the cell using TargetP, SignalP and related tools Nat Protoc 2007 2 4 953 971 10.1038/nprot.2007.131 17446895 

  14. Sakaguchi M Eukaryotic protein secretion Curr Opin Biotechnol 1997 8 5 595 601 10.1016/S0958-1669(97)80035-3 9353225 

  15. Borrero J Jimenez JJ Gutiez L Herranz C Cintas LM Hernandez PE Protein expression vector and secretion signal peptide optimization to drive the production, secretion, and functional expression of the bacteriocin enterocin A in lactic acid bacteria J Biotechnol 2011 156 1 76 86 10.1016/j.jbiotec.2011.07.038 21839785 

  16. Jonet MA Mahadi NM Murad AM Rabu A Bakar FD Rahim RA Low KO Illias RM Optimization of a heterologous signal peptide by site-directed mutagenesis for improved secretion of recombinant proteins in Escherichia coli J Mol Microbiol Biotechnol 2012 22 1 48 58 10.1159/000336524 22456489 

  17. Liu FC Chen HL Chong KY Hsu AL Chen CM Production of recombinant porcine colipase secreted by Pichia pastoris and its application to improve dietary fat digestion and growth of postweaning piglets Biotechnol Prog 2008 24 6 1333 1341 10.1002/btpr.46 19194948 

  18. Rakestraw JA Sazinsky SL Piatesi A Antipov E Wittrup KD Directed evolution of a secretory leader for the improved expression of heterologous proteins and full-length antibodies in Saccharomyces cerevisiae Biotechnol Bioeng 2009 103 6 1192 1201 10.1002/bit.22338 19459139 

  19. Futatsumori-Sugai M Tsumoto K Signal peptide design for improving recombinant protein secretion in the baculovirus expression vector system Biochem Biophys Res Commun 2009 391 1 931 935 19962965 

  20. de Wildt RM Mundy CR Gorick BD Tomlinson IM Antibody arrays for high-throughput screening of antibody-antigen interactions Nat Biotechnol 2000 18 9 989 994 10.1038/79494 10973222 

  21. Holt LJ Bussow K Walter G Tomlinson IM By-passing selection: direct screening for antibody-antigen interactions using protein arrays Nucleic Acids Res 2000 28 15 E72 10.1093/nar/28.15.e72 10908365 

  22. Nielsen H Engelbrecht J Brunak S von Heijne G A neural network method for identification of prokaryotic and eukaryotic signal peptides and prediction of their cleavage sites Int J Neural Syst 1997 8 5–6 581 599 10065837 

  23. Wiese M Ilg T Lottspeich F Overath P Ser/Thr-rich repetitive motifs as targets for phosphoglycan modifications in Leishmania mexicana secreted acid phosphatase EMBO J 1995 14 6 1067 1074 7720697 

  24. Dortay H Schmockel SM Fettke J Mueller-Roeber B Expression of human c-reactive protein in different systems and its purification from Leishmania tarentolae Protein Expr Purif 2011 78 1 55 60 10.1016/j.pep.2011.03.010 21440634 

  25. Sugino M Niimi T Expression of multisubunit proteins in Leishmania tarentolae Methods Mol Biol 2012 824 317 325 10.1007/978-1-61779-433-9_16 22160906 

  26. Choo KH Tan TW Ranganathan S A comprehensive assessment of N-terminal signal peptides prediction methods BMC Bioinforma 2009 10 Suppl 15 S2 10.1186/1471-2105-10-S15-S2 

  27. Petersen TN Brunak S von Heijne G Nielsen H SignalP 4.0: discriminating signal peptides from transmembrane regions Nat Methods 2011 8 10) 785 786 21959131 

  28. Jacquemin MG Desqueper BG Benhida A Vander Elst L Hoylaerts MF Bakkus M Thielemans K Arnout J Peerlinck K Gilles JG Mechanism and kinetics of factor VIII inactivation: study with an IgG4 monoclonal antibody derived from a hemophilia A patient with inhibitor Blood 1998 92 2 496 506 9657749 

  29. Bendtsen JD Nielsen H von Heijne G Brunak S Improved prediction of signal peptides: SignalP 3.0 J Mol Biol 2004 340 4 783 795 10.1016/j.jmb.2004.05.028 15223320 

  30. Lei SP Lin HC Wang SS Callaway J Wilcox G Characterization of the Erwinia carotovora pelB gene and its product pectate lyase J Bacteriol 1987 169 9 4379 4383 3040692 

  31. von Heijne G Patterns of amino acids near signal-sequence cleavage sites Eur J Biochem 1983 133 1 17 21 10.1111/j.1432-1033.1983.tb07424.x 6852022 

  32. von Heijne G Analysis of the distribution of charged residues in the N-terminal region of signal sequences: implications for protein export in prokaryotic and eukaryotic cells EMBO J 1984 3 10 2315 2318 6499832 

  33. von Heijne G How signal sequences maintain cleavage specificity J Mol Biol 1984 173 2 243 251 10.1016/0022-2836(84)90192-X 6423828 

  34. Edman P A method for the determination of amino acid sequence in peptides Arch Biochem 1949 22 3 475 18134557 

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