[논문]Effects of palytoxins extracted from Ostreopsis ovata on the oxidative stress and immune responses in Pacific white shrimp Litopenaeus vannamei

Cen, Jingyi; Cui, Lei; Duan, Yafei; Zhang, Hua; Lin, Yarou; Zheng, Jiping; Lu, Songhui

doi:10.1016/j.fsi.2019.11.001

[해외논문] Effects of palytoxins extracted from Ostreopsis ovata on the oxidative stress and immune responses in Pacific white shrimp Litopenaeus vannamei

Fish & shellfish immunology, v.95, 2019년, pp.670 - 678

Cen, Jingyi (Key Laboratory of Aquatic Eutrophication and Control of Harmful Algae Blooms of Guangdong Higher Education Institutes, College of Life Science and Technology, Jinan University) , Cui, Lei (Key Laboratory of Aquatic Eutrophication and Control of Harmful Algae Blooms of Guangdong Higher Education Institutes, College of Life Science and Technology, Jinan University) , Duan, Yafei (Key Laboratory of South China Sea Fishery Resources Exploitation & Utilization, Ministry of Agriculture and Rural Affairs, Guangdong Provincial Key Laboratory of Fishery Ecology and Environment, South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences) , Zhang, Hua (Key Laboratory of Aquatic Eutrophication and Control of Harmful Algae Blooms of Guangdong Higher Education Institutes, College of Life Science and Technology, Jinan University) , Lin, Yarou (Key Laboratory of Aquatic Eutrophication and Control of Harmful Algae Bloom) , Zheng, Jiping , Lu, Songhui

Abstract ▼ AI-Helper

Abstract Palytoxins (PLTXs) are a group of complex and poisonous marine natural products that are toxic to marine life and even human beings. In the present study, the oxidative stress and immune response in the hepatopancreas and gills of Litopenaeus vannamei were assessed for 72 h after injection with PLTX extracts. Chemical and physiological parameters, e.g., the respiratory burst (O2 −), activities of antioxidant enzymes, oxidative damage to lipids, carbonylation of proteins, and immune gene mRNA expression levels, were analysed. The results showed that the PLTX extract was not fatal to the shrimp but could reduce their mobility. The O2 − levels in the gills gradually increased after exposure to PLTX extracts and were significantly higher than those in the control from 6 to 72 h. The malondialdehyde content, lipid peroxidation, protein carbonyl levels, and total antioxidant capacity in the gills all peaked at 12 h. At the same time, the gills were loosely connected, there was a clear disintegration of the epithelial tissue, and the stratum corneum disappeared after 12 h. In addition, compared to those in the control group, the PLTX extract treatment increased the O2 − content, malondialdehyde content, lipid peroxidation, and protein carbonyl levels from 12 to 72 h, 24–48 h, 12–24 h, and 12–72 h after injection in the hepatopancreas of the shrimp, respectively. Both the Crustin and Toll gene expression levels significantly increased in the hepatopancreas compared to those in the control 6–72 h after injection of the toxin. In parallel, the expression levels of the manganese superoxide dismutase gene gradually decreased from 6 to 48 h and returned to normal levels after 72 h. Interestingly, the total antioxidant capacity also significantly increased compared to that in the control from 6 to 72 h. Our results indicate that although PLTX extracts cause lipid peroxidation and carbonylation of proteins in hepatopancreatic cells, leading to their damage, they did not cause a decrease in the total antioxidant capacity of the hepatopancreas. Highlights Palytoxin is a complex poisonous molecule found in the marine environment. Palytoxin was injected into the Pacific white shrimp Litopenaeus vannamei. Palytoxin was not lethal but caused shrimp immobility. Palytoxin increased markers of oxidative damage in the hepatopancreas. Palytoxin did not cause a decrease in the total antioxidant capacity of the hepatopancreas.

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