[논문]Detection and imaging of cathepsin L in cancer cells using the aggregation of conjugated polymer dots and magnetic nanoparticles

Kim, Daigeun; Lee, Yong-Deok; Jo, Seonyoung; Kim, Sehoon; Lee, Taek Seung

doi:10.1016/j.snb.2019.127641

[해외논문] Detection and imaging of cathepsin L in cancer cells using the aggregation of conjugated polymer dots and magnetic nanoparticles

Sensors and actuators. B, Chemical, v.307, 2020년, pp.127641 -

Kim, Daigeun (Organic and Optoelectronic Materials Laboratory, Department of Advanced Organic Materials and Textile System Engineering, Chungnam National University) , Lee, Yong-Deok (Center for Theragnosis, Korea Institute of Science and Technology) , Jo, Seonyoung (Organic and Optoelectronic Materials Laboratory, Department of Advanced Organic Materials and Textile System Engineering, Chungnam National University) , Kim, Sehoon (Center for Theragnosis, Korea Institute of Science and Technology) , Lee, Taek Seung (Organic and Optoelectronic Materials Laboratory, Department of Advanced Organic Materials and Textile System Engineering, Chungnam National University)

Abstract ▼ AI-Helper

Abstract New technique for cathepsin L detection and imaging was developed via controlling an aggregation between magnetic nanoparticles (MNPs) and conjugated polymer dots (CPdots). By changing the electrostatic surface charges of MNPs, the techniques provided two protocols: 1) fluorescence quenching and spin-spin relaxation (T2)-based magnetic resonance imaging (MRI); 2) fluorescence recovery for fluorescent imaging. By adding cathepsin L, the fluorescence and MRI relaxivity were altered by the formation of aggregates of CPdots and MNPs, inducing fluorescence quenching by MNP as well as a change in T2 relaxation. In addition, nonfluorescent, pre-aggregated nanohybrid (CPdots and MNPs) can be dissolved in the presence of cathepsin L, resulting in turn-on fluorescent detection and the imaging of cathepsin L in cancer cells and in mice with tumors. In all cases, the MNPs played important roles as the fluorescence quencher and as the MRI-signal generator. The surface of CPdots was covered with poly(L-lysine) (pLys) for the aggregation of CPdots and MNPs, in which pLys was specifically degraded by the enzymatic action of cathepsin L. The CPdots systems showed high sensitivity and selectivity toward cathepsin L, by which these systems can be considered as potential candidates for cathepsin L detection and imaging. Highlights A nanohybridized probe composed of magnetic particles and conjugated polymer dots was prepared. The selectivity was realized by specific interaction between cathepsin L and poly(L-lysine). A nanohybridized probe exhibited an excellent performance in vivo tumor imaging.

Keyword

참고문헌 (54)

J. Biol. Chem. Hwang 282 9556 2007 10.1074/jbc.M605510200 Cathepsin L expression is directed to secretory vesicles for enkephalin neuropeptide biosynthesis and secretion

상세보기
J. Neurochem. Funkelstein 106 384 2008 10.1111/j.1471-4159.2008.05408.x Cathepsin L participates in the production of neuropeptide Y in secretory vesicles, demonstrated by protease gene knockout and expression

상세보기
Int. Rev. Cytol. Collette 241 1 2004 10.1016/S0074-7696(04)41001-8 Biosynthesis and alternate targeting of the lysosomal cysteine protease Cathepsin L
Funkelstein 768 107 2011 The novel role of cathepsin L for neuropeptide production illustrated by research strategies in chemical biology with protease Gene knockout and expression
Biol. Chem. Sevenich 387 885 2006 10.1515/BC.2006.112 Human Cathepsin L rescues the neurodegeneration and lethality in Cathepsin B/L double-deficient mice

상세보기
Am. J. Pathol. Tobin 160 1807 2002 10.1016/S0002-9440(10)61127-3 The lysosomal protease Cathepsin L is an important regulator of keratinocyte and melanocyte differentiation during hair follicle morphogenesis and cycling

상세보기
Biochem. Chauhan 37 8584 1998 10.1021/bi972251z involvement of carboxy-terminal amino acids in secretion of human lysosomal protease Cathepsin L

상세보기
Chem. Soc. Rev. Leung 41 1911 2012 10.1039/C1CS15213K Gold and iron oxide hybrid nanocomposite materials

상세보기
Adv. Funct. Mater. Sun 27 2017 10.1002/adfm.201704079 Photosensitizers for two‐photon excited photodynamic therapy

상세보기
Chem. Rev. Lovell 110 2839 2010 10.1021/cr900236h Activatable photosensitizers for imaging and therapy

상세보기
Acc. Chem. Res. Pullen 51 3052 2018 10.1021/acs.accounts.8b00415 Mixed-ligand metal-organic frameworks and heteroleptic coordination cages as multifunctional scaffolds-A comparison

상세보기
Chem. Commun. Yu 50 11614 2014 10.1039/C4CC03984J Stimuli-responsive cancer therapy based on nanoparticles

상세보기
J. Mater. Chem. Gandhi 21 15698 2011 10.1039/c1jm12500a Superparamagnetic nanosystems based on iron oxide nanoparticles & mesoporous silica: synthesis & evaluation of their magnetic, relaxometric and biocompatability properties

상세보기
J. Control. Release Cha 155 152 2011 10.1016/j.jconrel.2011.07.019 Development of MRI/NIRF ‘activatable’ multimodal imaging probe based on iron oxide nanoparticles

상세보기
Polym. Chem. Wang 8 5795 2017 10.1039/C7PY01289F Ratiometric imaging of lysosomal hypochlorous acid enabled by FRET-based polymer dots

상세보기
Sens. Actuator B: Chem. Liu 298 2019 10.1016/j.snb.2019.126935 Block copolymer nanoparticles-based fluorescent sensor for ultrasensitive detection of tyrosinase activity and inhibitor

상세보기
Small Lin 10 3246 2014 10.1002/smll.201303593 Encapsulated Fe3O4/Ag complexed cores in hollow gold nanoshells for enhanced theranostic magnetic resonance imaging and photothermal therapy

상세보기
Adv. Mater. Jia 30 2018 A magnetofluorescent carbon dot assembly as an acidic H2O2‐driven oxygenerator to regulate tumor hypoxia for simultaneous bimodal imaging and enhanced photodynamic therapy
Biomaterials Zhao 143 1 2017 10.1016/j.biomaterials.2017.07.030 Simultaneous inhibition of growth and metastasis of hepatocellular carcinoma by Co-delivery of ursolic acid and sorafenib using lactobionic acid modified and pH-sensitive chitosan-conjugated mesoporous silica nanocomplex

상세보기
ACS Appl. Mater. Interfaces Wang 8 17166 2016 10.1021/acsami.6b05370 Charge-reversal APTES-Modified mesoporous silica nanoparticles with high drug loading and release controllability

상세보기
Adv. Sci. Jia 2 2015 10.1002/advs.201500108 Virus‐surface‐mimicking surface clustering of AuNPs onto DNA‐entrapped polymeric nanoparticle for enhanced cellular internalization and nanocluster‐induced NIR photothermal therapy

상세보기
Acc. Chem. Res. Murphy 41 1721 2008 10.1021/ar800035u Gold nanoparticles in biology: beyond toxicity to cellular imaging

상세보기
Nanomedicine Day 7 1133 2012 10.2217/nnm.11.189 Vascular-targeted photothermal therapy of an orthotopic murine glioma model

상세보기
Adv. Mater. Meng 31 2019 10.1002/adma.201900927 Light‐triggered in situ gelation to enable robust photodynamic‐immunotherapy by repeated stimulations

상세보기
Small Yoon 2 209 2006 10.1002/smll.200500360 Specific targeting, cell sorting, and bioimaging with smart magnetic silica core-shell nanomaterials

상세보기
Phys. Chem. Chem. Phys. Benyettou 13 10020 2011 10.1039/c0cp02034f A multimodal magnetic resonance imaging nanoplatform for cancer theranostics

상세보기
Adv. Drug Deliv. Rev. Chandra 63 1267 2011 10.1016/j.addr.2011.06.003 Oxide and hybrid nanostructures for therapeutic applications

상세보기
Adv. Mater. Zhu 31 2019 10.1002/adma.201900321 Near‐infrared‐II molecular dyes for Cancer imaging and surgery

상세보기
Chem. Rev. Gaitzsch 116 1053 2016 10.1021/acs.chemrev.5b00241 Engineering functional polymer capsules toward smart nanoreactors

상세보기
J. Magn. Magn. Mater. Shokrollahi 369 176 2014 10.1016/j.jmmm.2014.06.023 Magnetic resonance imaging by using nano-magnetic particles

상세보기
Nano Lett. Lu 7 149 2007 10.1021/nl0624263 Bifunctional magnetic silica nanoparticles for highly efficient human stem cell labeling

상세보기
J. Phys. Condens. Matter Holzapfel 18 S2581 2006 10.1088/0953-8984/18/38/S04 Synthesis and biomedical applications of functionalized fluorescent and magnetic dual reporter nanoparticles as obtained in the miniemulsion process

상세보기
Biomacromolecules Pfaff 12 3805 2011 10.1021/bm201051p Magnetic and fluorescent glycopolymer hybrid nanoparticles for intranuclear optical imaging

상세보기
Langmuir Wang 28 1657 2011 10.1021/la204494v Multifunctional magnetic mesoporous silica nanocomposites with improved sensing performance and effective removal ability toward Hg(II)

상세보기
Anal. Chem. Ali 83 2877 2011 10.1021/ac103261y Multifunctional nanoparticles for dual imaging

상세보기
Nano Lett. Wang 4 409 2004 10.1021/nl035010n Superparamagnetic Fe2O3 beads−CdSe/ZnS quantum dots core−shell nanocomposite particles for cell separation

상세보기
Langmuir Bertorelle 22 5385 2006 10.1021/la052710u Fluorescence-modified superparamagnetic nanoparticles: intracellular uptake and use in cellular imaging

상세보기
J. Am. Chem. Soc. Wu 132 15410 2010 10.1021/ja107196s Bioconjugation of ultrabright semiconducting polymer dots for specific cellular targeting

상세보기
ACS Nano Wu 2 2415 2008 10.1021/nn800590n Multicolor conjugated polymer dots for biological fluorescence imaging

상세보기
Adv. Mater. Kaeser 22 2985 2010 10.1002/adma.201000427 Fluorescent nanoparticles based on self‐assembled π‐conjugated systems

상세보기
Chem. Rev. Pecher 110 6260 2010 10.1021/cr100132y Nanoparticles of conjugated polymers

상세보기
Small Kim 14 2018 Emission tuning with size‐controllable polymer dots from a single conjugated polymer

상세보기
Chem. Commun. Chan 47 2820 2011 10.1039/c0cc04929h Copper(II) and Iron(II) ion sensing with semiconducting polymer dots

상세보기
ACS Appl. Mater. Interfaces Kim 8 34770 2016 10.1021/acsami.6b12277 Photoswitchable emission color change in nanodots containing conjugated polymer and photochrome

상세보기
J. Am. Chem. Soc. Wu 137 173 2015 10.1021/ja5123045 Squaraine-based polymer dots with narrow, bright near-infrared fluorescence for biological applications

상세보기
Sens. Actuator B: Chem. Kim 264 45 2018 10.1016/j.snb.2018.02.118 Dual-signal detection of trypsin using controlled aggregation of conjugated polymer dots and magnetic nanoparticles

상세보기
J. Mol. Pharm. Org. Process Res. Mehta 15 4568 2018 Reducing dendrimer generation and PEG chain length increases drug release and promotes anticancer activity of PE Gylated polylysine dendrimers conjugated with doxorubicin via a cathepsin-cleavable peptide linker
J. Control. Release Chu 157 445 2012 10.1016/j.jconrel.2011.10.016 Cathepsin B-sensitive polymers for compartment-specific degradation and nucleic acid release

상세보기
Oncol. Lett. Chen 2 693 2011 10.3892/ol.2011.302 Detection of Cathepsin B, Cathepsin L, Cystatin C, urokinase plasminogen activator and urokinase plasminogen activator receptor in the sera of lung cancer patients

상세보기
J. Colloid Sci. Biotechnol. Macková 1 235 2012 10.1166/jcsb.2012.1018 The use of magnetic poly(N-isopropylacrylamide) microspheres for separation of DNA from probiotic dairy products

상세보기
Dalton Trans. Smolensky 41 8039 2012 10.1039/c2dt30416c A responsive particulate MRI contrast agent for Copper(I): a cautionary Aale

상세보기
J. Am. Chem. Soc. Smolensky 135 8966 2013 10.1021/ja402107x Magnetoluminescent light switches - dual modality in DNA detection

상세보기
J. Magn. Magn. Mater. Roch 293 532 2005 10.1016/j.jmmm.2005.01.070 Superparamagnetic colloid suspensions: water magnetic relaxation and clustering

상세보기
Eur. J. Biochem. Brinker 216 5085 2000 10.1046/j.1432-1327.2000.01570.x Highly potent inhibitors of human cathepsin L identified by screening combinatorial pentapeptide amide collection

상세보기

LOADING...

활용도 분석정보

상세보기

다운로드

내보내기

활용도 Top5 논문

해당 논문의 주제분야에서 활용도가 높은 상위 5개 콘텐츠를 보여줍니다.
더보기 버튼을 클릭하시면 더 많은 관련자료를 살펴볼 수 있습니다.

표제어: PCR

동의어: Packet Collision Rate

용어 설명 출처 목록 (6)

용어 설명: PCR은 세균 특이성이 있는 primer를 이용하여 적은 수의 세균이 있을지라도 쉽게 검출할 수 있는 유용한 방법이며, 이를 이용하여 구강 내 치면세균막이나 타액에서 직접 세균을 검출할 수 있게 되었다[8].

내보내기 구분	파일저장 인쇄 메일전송
구성항목	기본정보 상세정보 관리번호, 논문명, 저널/프로시딩명, 저자 , 발행년, 권, 호, 시작페이지, 끝페이지, 발행기관 관리번호, 논문명, 대등논문명, 저자 , 저널/프로시딩명, 발행기관, 발행년, 발행언어, 권, 호, 시작페이지, 끝페이지, ISBN, ISSN, 주제분야, 키워드, 초록(한글), 초록(영문), 저자(소속기관)
저장형식	Text(ASCII format) Excel format RefWorks Direct Export RIS format (for Reference Manager, ProCite, EndNote), Scholar's Aids, Mendeley
메일정보	받는사람 (필수) @ 보내는사람 (선택) @ 제목 내용 KISTI 검색결과 이메일 서비스
안내	총 건의 자료가 검색되었습니다. 다운받으실 자료의 인덱스를 입력하세요. (1-10,000) 검색결과의 순서대로 최대 10,000건 까지 다운로드가 가능합니다. 데이타가 많을 경우 속도가 느려질 수 있습니다.(최대 2~3분 소요) 다운로드 파일은 UTF-8 형태로 저장됩니다. 파일의 내용이 제대로 보이지 않을실 때는 웹브라우저 상단의 보기 -> 인코딩 -> 자동선택 여부를 확인하십시오. ~ Text(ASCII format) Excel format

연합인증