[논문]Phase contrast microscopy of living cells within the whole lens: spatial correlations and morphological dynamics

Kong, Zhiying; Zhu, Xiangjia; Zhang, Shenghai; Wu, Jihong; Luo, Yi

Phase contrast microscopy of living cells within the whole lens: spatial correlations and morphological dynamics 원문보기

Molecular vision, v.18, 2012년, pp.2165 - 2173

Kong, Zhiying (Department of Ophthalmology, Eye & ENT Hospital, Fudan University, Shanghai, P.R. China) , Zhu, Xiangjia (Department of Ophthalmology, Eye & ENT Hospital, Fudan University, Shanghai, P.R. China) , Zhang, Shenghai (Experimental Research Center, Eye & ENT Hospital, Fudan University, Shanghai, P.R. China) , Wu, Jihong (Experimental Research Center, Eye & ENT Hospital, Fudan University, Shanghai, P.R. China) , Luo, Yi (Department of Ophthalmology, Eye & ENT Hospital, Fudan University, Shanghai, P.R. China)

Abstract ▼ AI-Helper

PurposeImages from cultured lens cells do not convey enough spatial information, and imaging of fixed lens specimens cannot reveal dynamic changes in the cells. As such, a real-time, convenient approach for monitoring label-free imaging of dynamic processes of living cells within the whole lens is urgently needed.MethodsFemale Wistar rat lenses were kept in organ culture. Insulin-like growth factor-I was added to the culture medium to induce cell mitosis. A novel method of ultraviolet (UV) irradiation was used to induce cell apoptosis and fiber damage. The cellular morphological dynamics within the whole lens were monitored by inverted phase contrast microscopy. Apoptosis was assessed using a commercial kit with Hoechst 33342/YO-PRO®-1/propidium iodide (PI).ResultsThe intrinsic transparency and low-light scattering property of the rat lens permitted direct imaging of the lens epithelial cells (LECs) and the superficial fiber cells. We visualized the processes of mitosis and apoptosis of the LECs, and we obtained dynamic images of posterior fiber cells following UVA irradiation.ConclusionsThis method opens a new window for observing lens cells in their physiologic location, and it can be readily applied in studies on lens physiology and pathology.

참고문헌 (25)

1 Taylor VL Al-Ghoul KJ Lane CW Davis VA Kuszak JR Costello MJ Morphology of the normal human lens. Invest Ophthalmol Vis Sci 1996 37 1396 410 8641842

상세보기
2 Schmolze DB Standley C Fogarty KE Fischer AH Advances in microscopy techniques. Arch Pathol Lab Med 2011 135 255 63 21284447

상세보기
3 Bantseev VL Herbert KL Trevithick JR Sivak JG Mitochondria of rat lenses: distribution near and at the sutures. Curr Eye Res 1999 19 506 16 10550793

상세보기
4 Ji N Shroff H Zhong H Betzig E Advances in the speed and resolution of light microscopy. Curr Opin Neurobiol 2008 18 605 16 19375302
5 Verschueren H Interference reflection microscopy in cell biology: methodology and applications. J Cell Sci 1985 75 279 301 3900106
6 Unwin PN Phase contrast and interference microscopy with the electron microscope. Philos Trans R Soc Lond B Biol Sci 1971 261 95 104 4399209
7 Delaye M Tardieu A Short-range order of crystallin proteins accounts for eye lens transparency. Nature 1983 302 415 7 6835373

상세보기
8 Bassnett S Shi Y Vrensen GF Biological glass: structural determinants of eye lens transparency. Philos Trans R Soc Lond B Biol Sci 2011 366 1250 64 21402584
9 Wormstone IM Wride MA The ocular lens: a classic model for development, physiology and disease. Philos Trans R Soc Lond B Biol Sci 2011 366 1190 2 21402579
10 Gierthy JF Borrow SN Rothstein H Microscopy of living epithelial cells upon the intact ocular lens in culture. Exp Cell Res 1968 50 476 9 4925966

상세보기
11 Sugiyama Y Prescott AR Tholozan F Ohno S Quinlan RA Expression and localisation of apical junctional complex proteins in lens epithelial cells. Exp Eye Res 2008 87 64 70 18508048

상세보기
12 Lo WK Harding CV Tight junctions in the lens epithelia of human and frog: freeze-fracture and protein tracer studies. Invest Ophthalmol Vis Sci 1983 24 396 402 6601087
13 McAvoy JW Cell division, cell elongation and distribution of alpha-, beta- and gamma-crystallins in the rat lens. J Embryol Exp Morphol 1978 44 149 65 650132

상세보기
14 Griep AE Cell cycle regulation in the developing lens. Semin Cell Dev Biol 2006 17 686 97 17218126

상세보기
15 Arnold DR Moshayedi P Schoen TJ Jones BE Chader GJ Waldbillig RJ Distribution of IGF-I and -II, IGF binding proteins (IGFBPs) and IGFBP mRNA in ocular fluids and tissues: potential sites of synthesis of IGFBPs in aqueous and vitreous. Exp Eye Res 1993 56 555 65 7684697

상세보기
16 Reddan JR Wilson-Dziedzic D Insulin growth factor and epidermal growth factor trigger mitosis in lenses cultured in a serum-free medium. Invest Ophthalmol Vis Sci 1983 24 409 16 6339437
17 Shui YB Sasaki H Pan JH Hata I Kojima M Yamada Y Hirai KI Takahashia N Sasaki K Morphological observation on cell death and phagocytosis induced by ultraviolet irradiation in a cultured human lens epithelial cell line. Exp Eye Res 2000 71 609 18 11095913

상세보기
18 Michael R Vrensen GF van Marle J Gan L Soderberg PG Apoptosis in the rat lens after in vivo threshold dose ultraviolet irradiation. Invest Ophthalmol Vis Sci 1998 39 2681 7 9856778
19 Varma SD Kovtun S Hegde KR Role of ultraviolet irradiation and oxidative stress in cataract formation-medical prevention by nutritional antioxidants and metabolic agonists. Eye Contact Lens 2011 37 233 45 21670697

상세보기
20 Mafia K Gupta R Kirk M Wilson L Srivastava OP Barnes S UV-A-induced structural and functional changes in human lens deamidated alphaB-crystallin. Mol Vis 2008 14 234 48 18334940
21 Williams DL Oxidation, antioxidants and cataract formation: a literature review. Vet Ophthalmol 2006 9 292 8 16939456
22 Balasubramanian D Bansal AK Basti S Bhatt KS Murthy JS Rao CM The biology of cataract. The Hyderabad Cataract Research Group. Indian J Ophthalmol 1993 41 153 71 8005647
23 Meyer LM Dong X Wegener A Soderberg P Light scattering in the C57BL/6 mouse lens. Acta Ophthalmol Scand 2007 85 178 82 17305731
24 Swamy MS Abraham EC Lens protein composition, glycation and high molecular weight aggregation in aging rats. Invest Ophthalmol Vis Sci 1987 28 1693 701 3654142
25 Massignani M Canton I Sun T Hearnden V Macneil S Blanazs A Armes SP Lewis A Battaglia G Enhanced fluorescence imaging of live cells by effective cytosolic delivery of probes. PLoS ONE 2010 5 e10459 20454666

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Phase contrast microscopy of living cells within the whole lens: spatial correlations and morphological dynamics 원문보기

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