[논문]An Alternative Approach to Detecting Cancer Cells by Multi-Directional Fluorescence Detection System Using Cost-Effective LED and Photodiode

Cho, Kyoungrae; Seo, Jeong-hyeok; Heo, Gyeongyong; Choe, Se-woon

doi:10.3390/s19102301

An Alternative Approach to Detecting Cancer Cells by Multi-Directional Fluorescence Detection System Using Cost-Effective LED and Photodiode 원문보기

Sensors, v.19 no.10, 2019년, pp.2301 -

Cho, Kyoungrae (Department of Medical IT Convergence Engineering, Kumoh National Institute of Technology, Gumi 39253, Korea) , Seo, Jeong-hyeok (jkl6464@kumoh.ac.kr (K.C.)) , Heo, Gyeongyong (whiteneco@yonsei.ac.kr (J.-h.S.)) , Choe, Se-woon (Department of Medical IT Convergence Engineering, Kumoh National Institute of Technology, Gumi 39253, Korea)

Abstract ▼ AI-Helper

The enumeration of cellular proliferation by covering from hemocytometer to flow cytometer is an important procedure in the study of cancer development. For example, hemocytometer has been popularly employed to perform manual cell counting. It is easily achieved at a low-cost, however, manual cell counting is labor-intensive and prone to error for a large number of cells. On the other hand, flow cytometer is a highly sophisticated instrument in biomedical and clinical research fields. It provides detailed physical parameters of fluorescently labeled single cells or micro-sized particles depending on the fluorescence characteristics of the target sample. Generally, optical setup to detect fluorescence uses a laser, dichroic filter, and photomultiplier tube as a light source, optical filter, and photodetector, respectively. These components are assembled to set up an instrument to measure the amount of scattering light from the target particle; however, these components are costly, bulky, and have limitations in selecting diverse fluorescence dyes. Moreover, they require multiple refined and expensive modules such as cooling or pumping systems. Thus, alternative cost-effective components have been intensively developed. In this study, a low-cost and miniaturized fluorescence detection system is proposed, i.e., costing less than 100 US dollars, which is customizable by a 3D printer and light source/filter/sensor operating at a specific wavelength using a light-emitting diode with a photodiode, which can be freely replaceable. The fluorescence detection system can quantify multi-directional scattering lights simultaneously from the fluorescently labeled cervical cancer cells. Linear regression was applied to the acquired fluorescence intensities, and excellent linear correlations (R2 > 0.9) were observed. In addition, the enumeration of the cells using hemocytometer to determine its performance accuracy was analyzed by Student’s t-test, and no statistically significant difference was found. Therefore, different cell concentrations are reversely calculated, and the system can provide a rapid and cost-effective alternative to commercial hemocytometer for live cell or microparticle counting.

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참고문헌 (20)

1. Absher M. Hemocytometer counting Tissue Culture Elsevier Amsterdam, The Netherlands 1973 395 397
2. Ahrberg C.D. Lee J.M. Chung B.G. Poisson statistics-mediated particle/cell counting in microwell arrays Sci. Rep. 2018 8 2438 10.1038/s41598-018-20913-0 29403088

상세보기
3. Robinson J.P. Roederer M. Flow cytometry strikes gold Science 2015 350 739 740 10.1126/science.aad6770 26564833

상세보기
4. Adan A. Alizada G. Kiraz Y. Baran Y. Nalbant A. Flow cytometry: Basic principles and applications Crit. Rev. Biotechnol. 2017 37 163 176 10.3109/07388551.2015.1128876 26767547

상세보기
5. Kaushik S. Magnetic Flow Cytometry for Point-of-Care Applications Available online: https://cloudfront.escholarship.org/dist/prd/content/qt45b287bv/qt45b287bv.pdf?t=ony7gq (accessed on 18 May 2019)
6. Cointe S. Judicone C. Robert S. Mooberry M.J. Poncelet P. Wauben M. Nieuwland R. Key N.S. Dignat-George F. Lacroix R. Standardization of microparticle enumeration across different flow cytometry platforms: Results of a multicenter collaborative workshop J. Thromb. Haemost. 2017 15 187 193 10.1111/jth.13514 27662257

상세보기
7. Kutschera A. Lamb J.J. Cost-Effective Live Cell Density Determination of Liquid Cultured Microorganisms Curr. Microbiol. 2018 75 231 236 10.1007/s00284-017-1370-3 29022067

상세보기
8. Wind L. Szymanski W. Quantification of scattering corrections to the Beer-Lambert law for transmittance measurements in turbid media Meas. Sci. Technol. 2002 13 270 10.1088/0957-0233/13/3/306

상세보기
9. Wilkerson M.J. Principles and Applications of Flow Cytometry and Cell Sorting in Companion Animal Medicine Vet. Clin. N. Am. Small Anim. Pract. 2012 42 53 71 10.1016/j.cvsm.2011.09.012 22285157

상세보기
10. Pospichalova V. Svoboda J. Dave Z. Kotrbova A. Kaiser K. Klemova D. Ilkovics L. Hampl A. Crha I. Jandakova E. Simplified protocol for flow cytometry analysis of fluorescently labeled exosomes and microvesicles using dedicated flow cytometer J. Extracell. Vesicles 2015 4 25530 10.3402/jev.v4.25530 25833224

상세보기
11. Yang F.-B. Pan J.-Z. Zhang T. Fang Q. A low-cost light-emitting diode induced fluorescence detector for capillary electrophoresis based on an orthogonal optical arrangement Talanta 2009 78 1155 1158 10.1016/j.talanta.2009.01.033 19269486

상세보기
12. Macka M. Piasecki T. Dasgupta P.K. Light-Emitting Diodes for Analytical Chemistry Annu. Rev. Anal. Chem. 2014 7 183 207 10.1146/annurev-anchem-071213-020059 24818811

상세보기
13. Geng X. Gao Y. Feng C. Guan Y. A facile and high sensitive micro fluorimeter based on light emitting diode and photodiode Talanta 2017 175 183 188 10.1016/j.talanta.2017.07.022 28841976

상세보기
14. Poncelet P. Robert S. Bailly N. Garnache-Ottou F. Bouriche T. Devalet B. Segatchian J.H. Saas P. Mullier F. Tips and tricks for flow cytometry-based analysis and counting of microparticles Transfus. Apher. Sci. 2015 53 110 126 10.1016/j.transci.2015.10.008 26603057

상세보기
15. Knowlton S. Joshi A. Syrrist P. Coskun A.F. Tasoglu S. 3D-printed smartphone-based point of care tool for fluorescence- and magnetophoresis-based cytometry Lab Chip 2017 17 2839 2851 10.1039/C7LC00706J 28726914

상세보기
16. Sintsov M. Suchkov D. Khazipov R. Minlebaev M. Improved Recordings of the Optical Intrinsic Signals in the Neonatal Rat Barrel Cortex BioNanoScience 2017 7 333 337 10.1007/s12668-016-0359-x

상세보기
17. Ammara N. Noreen J. Misfa T. Madiha M. Sammar N. Fluorescence Activated Cell Sorting (FACS): An Advanced Cell Sorting Technique PSM Biol. Res. 2017 2 83 88
18. Arneodo F. Benabderrahmane M.L. Dahal S. Di Giovanni A. Pazos Clemens L. Candela A. D’Incecco M. Sablone D. Franchi G. Muon tracking system with Silicon Photomultipliers Nucl. Instrum. Methods Phys. Res. Sect. A 2015 799 166 171 10.1016/j.nima.2015.08.003

상세보기
19. Choe S.-W. Park K. Park C. Ryu J. Choi H. Combinational light emitting diode-high frequency focused ultrasound treatment for HeLa cell Comput. Assist. Surg. 2017 22 79 85 10.1080/24699322.2017.1379158 28956464

상세보기
20. Cassens U. Gohde W. Kuling G. Groning A. Schlenke P. Lehman L.G. Traoré Y. Servais J. Henin Y. Reichelt D. Simplified volumetric flow cytometry allows feasible and accurate determination of CD4 T lymphocytes in immunodeficient patients worldwide Antivir. Ther. 2004 9 395 406 15259902

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An Alternative Approach to Detecting Cancer Cells by Multi-Directional Fluorescence Detection System Using Cost-Effective LED and Photodiode 원문보기

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An Alternative Approach to Detecting Cancer Cells by Multi-Directional Fluorescence Detection System Using Cost-Effective LED and Photodiode 원문보기

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