[논문]Inhibitory Effects of Raw-Extract Centella asiatica (RECA) on Acetylcholinesterase, Inflammations, and Oxidative Stress Activities via In Vitro and In Vivo

Hafiz, Zetty Zulikha; Amin, Muhammad ‘Afif Mohd; Johari James, Richard Muhammad; Teh, Lay Kek; Salleh, Mohd Zaki; Adenan, Mohd Ilham

doi:10.3390/molecules25040892

Inhibitory Effects of Raw-Extract Centella asiatica (RECA) on Acetylcholinesterase, Inflammations, and Oxidative Stress Activities via In Vitro and In Vivo 원문보기

Molecules a journal of synthetic chemistry and natural product chemistry, v.25 no.4, 2020년, pp.892 -

Hafiz, Zetty Zulikha (Faculty of Applied Sciences, Universiti Teknologi MARA, 40450 Shah Alam, Selangor Darul Ehsan, Malaysia) , Amin, Muhammad ‘Afif Mohd (zulikha87@gmail.com) , Johari James, Richard Muhammad (Integrative Pharmacogenomics Institute (iPROMISE), Universiti Teknologi MARA Selangor Branch, Puncak Alam Campus, 42300 Puncak Alam, Selangor Darul Ehsan, Malaysia richard@puncakalam.uitm.edu.my (R.M.J.J.)) , Teh, Lay Kek (tehlaykek@puncakalam.uitm.edu.my (L.K.T.)) , Salleh, Mohd Zaki (zakisalleh@puncakalam.uitm.edu.my (M.Z.S.)) , Adenan, Mohd Ilham (Integrative Pharmacogenomics Institute (iPROMISE), Universiti Teknologi MARA Selangor Branch, Puncak Alam Campus, 42300 Puncak Alam, Selangor Darul Ehsan, Malaysia richard@puncakalam.uitm.edu.my (R.M.J.J.))

Abstract ▼ AI-Helper

Centella asiatica (C. asiatica) is one of the medicinal plants that has been reported to exert comprehensive neuroprotection in vitro and in vivo. In view of this, the present study was performed to investigate the effect of ethanolic extract of C. asiatica, designated as raw-extract of C. asiatica (RECA) in reducing the acetylcholinesterase (AChE), inflammations, and oxidative stress activities via both in vitro (SH-SY5Y and RAW 264.7 cells) and in vivo (Sprague Dawley rats). Quantitative high-performance liquid chromatography analysis reveals that RECA contains a significantly high proportion of glycosides than the aglycones with madecassoside as the highest component, followed by asiaticoside. Treatment of SH-SY5Y cells with RECA significantly reduced the AChE activity in a concentration-dependent manner with an IC50 value of 31.09 ± 10.07 µg/mL. Furthermore, the anti-inflammatory and antioxidant effects of RECA were evaluated by lipopolysaccharides (LPS)-stimulated RAW 264.7 cells. Our results elucidated that treatment with RECA significantly suppressed the level of pro-inflammatory cytokine/mediators and oxidative stress released in a concentration-dependent manner. Interestingly, these patterns of inhibition were consistent as observed in the LPS-induced neuroinflammation Sprague Dawley rats’ model. The highest concentration used in the two models presented the most significant results. Herein, our findings strongly suggest that RECA may offer therapeutic potential for the treatment of Alzheimer’s disease through inhibiting the AChE, inflammation, and oxidative stress activities.

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참고문헌 (75)

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표제어: PCR

동의어: Packet Collision Rate

용어 설명 출처 목록 (6)

용어 설명: PCR은 세균 특이성이 있는 primer를 이용하여 적은 수의 세균이 있을지라도 쉽게 검출할 수 있는 유용한 방법이며, 이를 이용하여 구강 내 치면세균막이나 타액에서 직접 세균을 검출할 수 있게 되었다[8].

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Inhibitory Effects of Raw-Extract Centella asiatica (RECA) on Acetylcholinesterase, Inflammations, and Oxidative Stress Activities via In Vitro and In Vivo 원문보기

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