[논문]Apigenin-7-O-glucoside versus apigenin: Insight into the modes of anticandidal and cytotoxic actions

Smiljkovic, Marija; Stanisavljevic, Danijela; Stojkovic, Dejan; Petrovic, Isidora; Marjanovic Vicentic, Jelena; Popovic, Jelena; Golic Grdadolnik, Simona; Markovic, Dejan; Sankovic-Babice, Snež; ana; Glamoclija, Jasmina; Stevanovic, Milena; Sokovic, Marina

doi:10.17179/excli2017-300

Apigenin-7-O-glucoside versus apigenin: Insight into the modes of anticandidal and cytotoxic actions 원문보기

EXCLI journal : experimental and clinical sciences, v.16, 2017년, pp.795 - 807

Smiljkovic, Marija (Department of Plant Physiology, Institute for Biological Research "Siniš) , Stanisavljevic, Danijela (a Stankovic", University of Belgrade, Bulevar Despota Stefana 142, 11000 Belgrade, Serbia) , Stojkovic, Dejan (Institute of Molecular Genetics and Genetic Engineering, University of Belgrade, Vojvode Stepe 444a, PO Box 23, 11010 Belgrade, Serbia) , Petrovic, Isidora (Department of Plant Physiology, Institute for Biological Research "Siniš) , Marjanovic Vicentic, Jelena (a Stankovic", University of Belgrade, Bulevar Despota Stefana 142, 11000 Belgrade, Serbia) , Popovic, Jelena (Institute of Molecular Genetics and Genetic Engineering, University of Belgrade, Vojvode Stepe 444a, PO Box 23, 11010 Belgrade, Serbia) , Golic Grdadolnik, Simona (Institute of Molecular Genetics and Genetic Engineering, University of Belgrade, Vojvode Stepe 444a, PO Box 23, 11010 Belgrade, Serbia) , Markovic, Dejan (Institute of Molecular Genetics and Genetic Engineering, University of Belgrade, Vojvode Stepe 444a, PO Box 23, 11010 Belgrade, Serbia) , Sankovic-Babice, Snežana (Laboratory of Biomolecular Structure, National Institute of Chemistry, Hajdrihova 19, 1000 Ljubljana, Slovenia) , Glamoclija, Jasmina , Stevanovic, Milena , Sokovic, Marina

Abstract ▼ AI-Helper

Bioactive potential of apigenin derivative apigenin-7-O-glucoside related to its antifungal activity on Candida spp. and cytotoxic effect on colon cancer cells was studied and compared with bioactive potential of apigenin. Antifungal activity was tested on 14 different isolates of Candida spp. using membrane permeability assay, measuring inhibition of reactive oxidative species and inhibition of CYP51 C. albicans enzyme. Cytotoxic potential of apigenin-7-O-glucoside was tested on colon cancer HCT116 cells by measuring cell viability, apoptosis rate and apoptosis- and colon cancer-related gene expression. Obtained results indicated considerable antifungal activity of apigenin-7-O-glucoside towards all Candida isolates. Breakdown of C. albicans plasma membrane was achieved upon treatment with apigenin-7-O-glucoside for shorter period of time then with apigenin. Reduction of intra- and extracellular reactive oxidative species was achieved with minimum inhibitory concentrations of both compounds, suggesting that reactive oxidative species inhibition could be a mechanism of antifungal action. None of the compounds exhibited binding affinity to C. albicans CYP51 protein. Besides, apigenin-7-O-glucoside was more effective compared to apigenin in reduction of cell's viability and induction of cell death of HCT116 cells. Treatment with both compounds resulted in chromatin condensation, apoptotic bodies formation and apoptotic genes expression in HCT116 cells, but the apigenin-7-O-glucoside required a lower concentration to achieve the same effect. Compounds apigenin-7-O-glucoside and apigenin displayed prominent antifungal potential and cytotoxic effect on HCT116 cells. However, our results showed that apigenin-7-O-glucoside has more potent activity compared to apigenin in all assays that we used.

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참고문헌 (38)

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Apigenin-7-O-glucoside versus apigenin: Insight into the modes of anticandidal and cytotoxic actions 원문보기

Abstract ▼ AI-Helper

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참고문헌 (38)

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