초록

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Agrobacterium sp.에 의해 생산되는 커들란은 2차 부산물이기 때문에 이의 생산을 증대시키기 위해서는 고농도 세포배양이 필요하다. 본 연구에서는 고농도 세포배양을 실현하기 위하여 탄소원 과 질소원을 제어 공급하는 방법을 이용하였다. 초기 탄소원의 농도가 20g/L일 때 24시간 내에 모두 소모되었으며 생산된 세포의 농도는 6g/L였다. 고농도의 탄소원을 이용하여 배양조내의 설탕의 농도가 10g/L 이상이 되도록 제어하는 방법을 사용하였다.

Abstract ▼ AI-Helper

Agrobacterium sp. ATCC 31750( formerly Alcaligenes faecalis subsp myxogenes) was used to produce curdlan. Since the curdlan is secondary metabolite, it is important for curdlan production to increase cell concentration. The fedbatch operation was used to increase cell concentration with addition of ...

Agrobacterium sp. ATCC 31750( formerly Alcaligenes faecalis subsp myxogenes) was used to produce curdlan. Since the curdlan is secondary metabolite, it is important for curdlan production to increase cell concentration. The fedbatch operation was used to increase cell concentration with addition of carbon and nitrogen sources. When the initial sucrose concentration was 20g/L, it was consumed in 24 hrs and the cell concentration was 6g/L in a batch culture. The sucrose solution(200g/L) was fed to control the sucrose concentration above 10g/L.