초록 ▼

An aptamer-based solid-state electrochemical biosensor for label-free detection of Salmonella enterica serovars utilizing immobilized aptamers. The device is realized by forming a matrix array of parallel capacitors, thus allowing the realization of low-cost, portable, fully integrated devices. Prot

An aptamer-based solid-state electrochemical biosensor for label-free detection of Salmonella enterica serovars utilizing immobilized aptamers. The device is realized by forming a matrix array of parallel capacitors, thus allowing the realization of low-cost, portable, fully integrated devices. Protein-aptamer binding modulates the threshold voltage of a circuit, changing the impedance (capacitance) of the circuit. This circuit is further characterized by an electrode coded with a p-Si substrate, enhancing the affinity between the Salmonella outer membrane proteins (OMPs) and the aptamer. An aptamer embedded detection plate is configured within a testing lid device that fits a standard, commercially available polymer specimen jar. A sample is mixed with broth for incubation and cultivation of any present Salmonella bacteria to obtain acceptable concentration of the pathogen for testing. The information obtained can then be transmitted by wireless network.

대표청구항 ▼

We claim: 1. An apparatus for detecting Salmonella enterica bacteria using an electrochemical aptamer-based sensor array comprising:a sensor base plate;a capacitive array disposed on the sensor base plate in a plurality of grooved channels;a plurality of aptamer molecules disposed within the capacit

We claim: 1. An apparatus for detecting Salmonella enterica bacteria using an electrochemical aptamer-based sensor array comprising:a sensor base plate;a capacitive array disposed on the sensor base plate in a plurality of grooved channels;a plurality of aptamer molecules disposed within the capacitive array, each of the plurality of aptamer molecules capable of binding to an indicator protein on an outer membrane of the Salmonella enterica, and wherein each aptamer molecule is immobilized to the capacitive array utilizing the Self-Assembled Monolayer (SAM) method; anda printed circuit board coupled to the capacitive array, the printed circuit board comprising means of interfacing and controlling the capacitive array, andwherein the printed circuit board and capacitive array are powered directly or indirectly to an independent power supply through a USB connection.