[미국특허]
TISSUE ENGINEERING DEVICE AND CONSTRUCTION OF VASCULARIZED DERMIS
원문보기
IPC분류정보
국가/구분
United States(US) Patent
공개
국제특허분류(IPC7판)
B05D-005/00
A61F-002/10
출원번호
US-0766466
(2013-02-13)
공개번호
US-0228970
(2014-08-14)
발명자
/ 주소
Boland, Thomas
출원인 / 주소
Boland, Thomas
인용정보
피인용 횟수 :
0인용 특허 :
0
초록▼
An inkjet printing method, system, and computer-usable tangible storage device to print cells and biomaterials for three-dimensional cellular scaffolds and engineered skin grafts are disclosed. The process simultaneously deposits living cells, nutrients, growth factors, therapeutic drugs along with
An inkjet printing method, system, and computer-usable tangible storage device to print cells and biomaterials for three-dimensional cellular scaffolds and engineered skin grafts are disclosed. The process simultaneously deposits living cells, nutrients, growth factors, therapeutic drugs along with biomaterial scaffolds at the right time and location. This technology can also be used for the microvasculature fabrication using appropriate human microvascular endothelial cells and fibrin to form the microvasculature. When printing human microvascular endothelial cells in conjunction with the fibrin, the cells aligned themselves inside the channels and proliferated to form confluent linings. The 3D tubular structure was also found in the printed patterns. Simultaneously printing biological materials to form a three-dimensional cellular scaffold promotes human microvascular endothelial cell proliferation and microvasculature formation.
대표청구항▼
1. A computer-implemented method for rendering a microvasculature, said method comprising: rendering biological ink via a rendering device;rendering a material along with said biological ink via said rendering device; andaligning said rendered biological ink and said rendered material into fibrin ch
1. A computer-implemented method for rendering a microvasculature, said method comprising: rendering biological ink via a rendering device;rendering a material along with said biological ink via said rendering device; andaligning said rendered biological ink and said rendered material into fibrin channels wherein said biological ink proliferates in a presence of said rendered material to form said microvasculature. 2. The computer-implemented method of claim 1, wherein said biological ink comprises human microvascular endothelial cells and fibrin, and wherein said material comprises at least one of living cells, a nutrient, a growth factors, a therapeutic drug. 3. The computer-implemented method of claim 1, wherein said rendering device comprises an ink jet printer, wherein a printer cartridge of said ink jet printer is modified to accommodate said biological ink and said material. 4. The computer-implemented method of claim 2, wherein said rendering said material along with said biological ink via said rendering device further comprises simultaneously depositing at least one of living cells, a nutrient, a growth factor, and a therapeutic drug along with said human microvascular endothelial cells and fibrin via said rendering device. 5. The computer-implemented method of claim 2, wherein said aligning said human microvascular endothelial cells and said fibrin inside fibrin channels further comprises utilizing drop-on-demand polymerization wherein said human microvascular endothelial cells and fibrin proliferate, and wherein said microvasculature is functional and comprises a confluent lining and a three-dimensional cellular scaffold. 6. The computer-implemented method of claim 2, further comprising heating said human microvascular endothelial cells, said fibrin, and said material to a preferable temperature between four degrees Celsius and thirty degrees Celsius above ambient temperature during rendering, wherein energy supplied during said rendering is transferred into kinetic energy to heat said human microvascular endothelial cells, said fibrin, and said material. 7. The computer-implemented method of claim 1, further comprising: supporting growth of multiple types of cells via a vasculature, said vasculature comprising at least one fibroblasts, interstitial fibroblasts, keratinocytes, melanocytes, adipose cells, adipose tissue-derived stem cells, cells from allogeneic or autologous sources;rendering a multiple-layer tissue construct, wherein said multiple-layer tissue construct comprises a plurality of densities and cell types, and wherein said multiple-layer tissue construct comprises at least one of a skin construct, an adipose construct, and a combination skin construct and adipose construct; andrendering at least one of a size match wound dressing, tissue replacement construct, and skin graft. 8. A system for rendering a microvasculature, comprising: a processor;a data bus coupled to said processor; anda computer-usable tangible storage device storing computer program code, said computer program code comprising program instructions executable by said processor, said program instructions comprising: program instructions to render biological ink via a rendering device;program instructions to render a material along with said biological ink via said rendering device; andprogram instructions to align said rendered biological ink and said rendered material into fibrin channels wherein said biological ink proliferates in a presence of said rendered material to form said microvasculature. 9. The system of claim 8, wherein said biological ink comprises human microvascular endothelial cells and fibrin. 10. The system of claim 8, wherein said rendering device comprises an ink jet printer, wherein a printer cartridge of said ink jet printer is modified to accommodate said biological ink and said material. 11. The system of claim 8, wherein said material comprises at least one of living cells, a nutrient, a growth factors, a therapeutic drug. 12. The system of claim 9, wherein said program instruction to render said material along with said biological ink via said rendering device further comprises program instructions to simultaneously deposit at least one of living cells, a nutrient, a growth factor, and a therapeutic drug along with said human microvascular endothelial cells and fibrin via said rendering device. 13. The system of claim 9, wherein said program instruction to align said human microvascular endothelial cells and said fibrin inside fibrin channels further comprises program instructions to utilize drop-on-demand polymerization wherein said human microvascular endothelial cells and fibrin proliferate, and wherein said microvasculature is functional and comprises a confluent lining and a three-dimensional cellular scaffold. 14. The system of claim 9, further comprising program instructions to heat said human microvascular endothelial cells, said fibrin, and said material to a preferable temperature between four degrees Celsius and thirty degrees Celsius above ambient temperature during rendering, wherein energy supplied during said rendering is transferred into kinetic energy to heat said human microvascular endothelial cells, said fibrin, and said material. 15. The system of claim 8, further comprising: program instructions to support growth of multiple types of cells via a vasculature, said vasculature comprising at least one fibroblasts, interstitial fibroblasts, keratinocytes, melanocytes, adipose cells, adipose tissue-derived stem cells, cells from allogeneic or autologous sources;program instructions to render a multiple-layer tissue construct, wherein said multiple-layer tissue construct comprises a plurality of densities and cell types, and wherein said multiple-layer tissue construct comprises at least one of a skin construct, an adipose construct, and a combination skin construct and adipose construct; andprogram instructions to render at least one of a size match wound dressing, tissue replacement construct, and skin graft. 16. A computer-usable tangible storage device storing computer program code for rendering a microvasculature, said computer program code comprising program instructions executable by a processor, said program instructions comprising: program instructions to render biological ink via a rendering device;program instructions to render a material along with said biological ink via said rendering device; andprogram instructions to align said rendered biological ink and said rendered material into fibrin channels wherein said biological ink proliferates in a presence of said rendered material to form said microvasculature. 17. The computer-usable tangible storage device of claim 16, wherein said program instruction to render said material along with said biological ink via said rendering device further comprises program instructions to simultaneously deposit at least one of living cells, a nutrient, a growth factor, and a therapeutic drug along with human microvascular endothelial cells and fibrin via said rendering device. 18. The computer-usable tangible storage device of claim 17, wherein said program instruction to align said human microvascular endothelial cells and said fibrin inside fibrin channels further comprises program instructions to utilize drop-on-demand polymerization wherein said human microvascular endothelial cells and fibrin proliferate, and wherein said microvasculature is functional and comprises a confluent lining and a three-dimensional cellular scaffold. 19. The computer-usable tangible storage device of claim 17, further comprising program instructions to heat said human microvascular endothelial cells, said fibrin, and said material to a preferable temperature between four degrees Celsius and thirty degrees Celsius above ambient temperature during rendering, wherein energy supplied during said rendering is transferred into kinetic energy to heat said human microvascular endothelial cells, said fibrin, and said material. 20. The computer-usable tangible storage device of claim 16, further comprising: program instructions to support growth of multiple types of cells via a vasculature, said vasculature comprising at least one fibroblasts, interstitial fibroblasts, keratinocytes, melanocytes, adipose cells, adipose tissue-derived stem cells, cells from allogeneic or autologous sources;program instructions to render a multiple-layer tissue construct, wherein said multiple-layer tissue construct comprises a plurality of densities and cell types, and wherein said multiple-layer tissue construct comprises at least one of a skin construct, an adipose construct, and a combination skin construct and adipose construct; andprogram instructions to render at least one of a size match wound dressing, tissue replacement construct, and skin graft. 21. An engineered adipose tissue graft, comprising: a plurality of cells and biomaterials in an engineered fibrin scaffold;tube-like channels within said cellular scaffold; anda plurality of microvascular channels within said fibrin for vascularization of said engineered adipose tissue graft. 22. The engineered adipose tissue graft of claim 21 wherein said plurality of cells comprises at least one of fibroblasts, interstitial fibroblasts, keratinocytes, melanocytes, adipose cells, adipose tissue-derived stem cells, cells from an allogenic source, and cells from a autologous source. 23. The engineered adipose tissue graft of claim 21 wherein said biomaterials comprise at least one of living cells, a nutrient, a growth factor, a therapeutic drug. 24. The engineered adipose tissue graft of claim 21 wherein said vasculature comprises at least one of fibroblasts, interstitial fibroblasts, keratinocytes, melanocytes, adipose cells, adipose tissue-derived stern cells, cells from an allogenic source, and cells from a autologous source. 25. The engineered adipose tissue graft of claim 21 wherein said plurality of microvascular channels are interconnected and of dimensions less than 200 micrometers in diameter.
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