IPC분류정보
국가/구분 |
United States(US) Patent
공개
|
국제특허분류(IPC7판) |
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출원번호 |
US-0297842
(2014-06-06)
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공개번호 |
US-0287428
(2014-09-25)
|
우선권정보 |
EP-11192838.8 (2011-12-09) |
발명자
/ 주소 |
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출원인 / 주소 |
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인용정보 |
피인용 횟수 :
0 인용 특허 :
0 |
초록
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A method of detecting the presence of bacteria in milk is provided, wherein milk is contacted with a preparation which may comprise an effective amount of at least one type of antibodies, said antibodies being capable of specifically binding to bacteria to be detected; staining said antibodies, befo
A method of detecting the presence of bacteria in milk is provided, wherein milk is contacted with a preparation which may comprise an effective amount of at least one type of antibodies, said antibodies being capable of specifically binding to bacteria to be detected; staining said antibodies, before or after said contacting, with a staining preparation, said staining preparation being selected so that the antibodies stained therewith exhibit a color change or a change in color concentration when in contact with said bacteria; and determining the concentration of stained antibodies bound to bacteria in said milk from the presence and/or the relative intensity of the color change caused by contacting said milk with said stained antibodies.
대표청구항
▼
1. A method of detecting the presence of bacteria in milk, wherein milk is contacted with a preparation comprising an effective amount of at least one type of antibodies, said antibodies being capable of specifically binding to bacteria to be detected; staining said antibodies, before or after said
1. A method of detecting the presence of bacteria in milk, wherein milk is contacted with a preparation comprising an effective amount of at least one type of antibodies, said antibodies being capable of specifically binding to bacteria to be detected; staining said antibodies, before or after said contacting, with a staining preparation, said staining preparation being selected so that the antibodies stained therewith exhibit a color change or a change in color concentration when in contact with said bacteria; anddetermining the concentration of stained antibodies bound to bacteria in said milk from the presence and/or the relative intensity of the color change caused by contacting said milk with said stained antibodies. 2. The method of claim 1, wherein said antibodies are capable of specifically binding to bacteria involved in mastitis. 3. The method of claim 2, wherein said preparation comprises more than one different type of antibodies, each type specifically binding to a different type of bacteria or a plurality of such types. 4. The method of claim 3, wherein said preparation comprises more than two different types of antibodies, each type specifically binding to a different type of bacteria or a plurality of such types. 5. The method of claim 4, wherein said preparation comprises more than three different types of antibodies, each type specifically binding to a different type of bacteria or a plurality of such types. 6. The method of claim 5, wherein said preparation comprises more than four different types of antibodies, each type specifically binding to a different type of bacteria or a plurality of such types. 7. The method of claim 6, wherein said preparation comprises more than five different types of antibodies, each type specifically binding to a different type of bacteria or a plurality of such types. 8. The method of claim 1, wherein the color change is detected, qualitatively and/or quantitatively, without separating the bacteria from the milk, preferably by using a fluorescent antibody stain and a detector such as a flow cytometer. 9. The method of claim 1, wherein the color change is detected, qualitatively and/or quantitatively, after separating the bacteria from the milk. 10. The method of claim 9, wherein the separating the bacteria from the milk is by micro-)filtration or centrifugation. 11. The method of claim 1, wherein the color change determines the type or types of bacteria in the milk. 12. The method of claim 11, wherein the color change also determines the relative amount (concentration) of said bacteria. 13. The method of claim 1, wherein prior to the color change detection step, the milk is rendered substantially clear. 14. The method of claim 13, wherein the milk is rendered substantially clear by partly or fully removing its protein and/or fatty components.
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