IPC분류정보
국가/구분 |
United States(US) Patent
공개
|
국제특허분류(IPC7판) |
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출원번호 |
US-0727442
(2015-06-01)
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공개번호 |
US-0053311
(2016-02-25)
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발명자
/ 주소 |
- BORMANN CHUNG, Christina
- Mulero, Julio
- Hennessy, Lori
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출원인 / 주소 |
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인용정보 |
피인용 횟수 :
0 인용 특허 :
0 |
초록
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Novel Y-STR multiplex analysis designs, primer design, allelic ladders, methods of use and kits are disclosed, including the use of primer sets designed to provide amplicons for at least 11 Y-STR loci having a base pair size of less than about 220 bp, as well as the use of primer sets designed to pr
Novel Y-STR multiplex analysis designs, primer design, allelic ladders, methods of use and kits are disclosed, including the use of primer sets designed to provide amplicons for at least 11 Y-STR loci having a base pair size of less than about 220 bp, as well as the use of primer sets designed to provide amplicons for at least 22 Y-STR loci including at least 5 rapidly mutating loci.
대표청구항
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1-17. (canceled) 18. A method of amplifying alleles of Y-STR markers of a human male comprising the steps of: contacting a sample suspected to contain a DNA sample of a human male with a set of amplification primers comprising primers for the amplification of the alleles of at least 11 Y-STR markers
1-17. (canceled) 18. A method of amplifying alleles of Y-STR markers of a human male comprising the steps of: contacting a sample suspected to contain a DNA sample of a human male with a set of amplification primers comprising primers for the amplification of the alleles of at least 11 Y-STR markers; andamplifying the sample thereby forming a plurality of sets of amplicons of the at least 11 Y-STR markers wherein each set of the amplicons has a base pair size less than 220 base pairs. 19. The method of claim 18, further comprising the step of detecting each set of amplicons whereby the alleles of the at least 11 Y-STR markers are identified. 20. The method of claim 18, wherein the detecting is performed by separating the plurality of sets of amplicons using a mobility dependent analysis. 21. The method of claim 18, wherein the at least 11 Y-STR markers are DYS576, DYS389I, DYS460, DYS458, DYS19, DYS456, DYS390, DYS570, DYS437, DYS393, and DYS439. 22. The method of claim 21, wherein the set of amplification primers consist of primers for the amplification of alleles of 27 Y-STR markers. 23. The method of claim 18, further comprising an additional set of primers for the amplification of more than the 11 Y-STR markers, wherein the additional set of primers are configured to provide sets of amplicons of the more than 11 Y-STR markers having a base pair size less than 410 base pairs. 24. The method of claim 22, wherein the 27 Y-STR markers are DYF387S1ab, DYS19, DYS385ab, DYS389I, DYS389II, DYS390, DYS391, DYS392, DYS393, DYS460, DYS437, DYS438, DYS439, DYS448, DYS449, DYS456, DYS458, DYS481, DYS518, DYS533, DYS570, DYS576, DYS627, DYS635, and Y-GATA-H4. 25. The method of claim 22, wherein the 27 Y-STR markers are DYF387S1ab, DYS19, DYS385ab, DYS389I, DYS389II, DYS390, DYS391, DYS392, DYS393, DYS460, DYS437, DYS438, DYS439, DYS448, DYS449, DYS456, DYS458, DYS481, DYS533, DYS570, DYS576, DYS627, DYS635, DYS643, and Y-GATA-H4. 26. The method of claim 20, wherein the mobility-dependent analytical technique is capillary electrophoresis. 27. The method of claim 18, wherein the detecting is performed by separating the plurality of sets of amplicons using a mobility dependent analysis, wherein the plurality of sets of amplicons are fluorescently labeled 28. The method of claim 18, wherein the detection of the amplicon base pair size is performed by a sequencing technique using no detection of fluorescent dye labels. 29. The method of claim 18, wherein each set of the amplicons of the at least 11 Y-STR markers is labeled with one of at least 5 dyes. 30. The method of claim 29, wherein the at least 5 dyes are fluorescent dyes configured to be spectrally distinct. 31. The method of claim 18, wherein at least one set of amplicons of the at least 11 Y-STR markers comprises a mobility modifier. 32. The method of claim 21, further comprising at least 5 Y-STR markers which are rapidly mutating loci. 33. The method of claim 32, wherein the at least 5 rapidly mutating Y-STR markers comprise DYF387S1ab, DYS449, DYS570, DYS576, and DYS627. 34. The method of claim 33, wherein the at least 5 rapidly mutating Y-STR markers further comprise DYS518. 35. The method of claim 18, wherein the set of primers for the amplification of at least 11 Y-STR markers is a set of 25 primers for the amplification of DYF387S1ab, DYS19, DYS385ab, DYS389I, DYS389II, DYS390, DYS391, DYS392, DYS393, DYS460, DYS437, DYS438, DYS439, DYS448, DYS449, DYS456, DYS458, DYS481, DYS518, DYS533, DYS570, DYS576, DYS627, DYS635, and Y-GATA-H4. 36. The method of claim 18, wherein the set of primers for the amplification of at least 11 Y-STR markers is a set of 25 primers for the amplification of DYF387S1ab, DYS19, DYS385ab, DYS389I, DYS389II, DYS390, DYS391, DYS392, DYS393, DYS460, DYS437, DYS438, DYS439, DYS448, DYS449, DYS456, DYS458, DYS481, DYS533, DYS570, DYS576, DYS627, DYS635, DYS643, and Y-GATA-H4. 37. The method of claim 18, performed using a kit comprising: a) the primers of claim 21; andb) a size standard comprising an allelic ladder.
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