The specification provides methods for isolating extracellular vesicles. Extracellular vesicles can be efficiently isolated, e.g., from biological fluids or cell culture media, using a heparin-coated solid support.
대표청구항▼
1. A method of isolating an extracellular vesicle (EV), the method comprising: providing a sample comprising an EV;contacting the sample with a heparin-coated solid support under conditions that allow the solid support to bind to the EV; andseparating the solid support-bound EV from the sample, ther
1. A method of isolating an extracellular vesicle (EV), the method comprising: providing a sample comprising an EV;contacting the sample with a heparin-coated solid support under conditions that allow the solid support to bind to the EV; andseparating the solid support-bound EV from the sample, thereby isolating the EV from the sample. 2. The method of claim 1, wherein the sample comprises biological fluid. 3. The method of claim 1, wherein the sample comprises human biological fluid. 4. The method of claim 1, wherein the sample comprises urine, mucus, saliva, tears, blood, serum, plasma, sputum, cerebrospinal fluid, ascites fluid, semen, lymph fluid, airway fluid, intestinal fluid, breast milk, amniotic fluid, or any combination thereof. 5. The method of claim 1, wherein the sample comprises cell culture medium. 6. The method of claim 1, wherein the solid support is a bead. 7. The method of claim 1, wherein the solid support is selected from the group consisting of an agarose bead, a magnetic bead, a silica bead, a polystyrene plate, a polystyrene bead, a glass bead, a cellulose bead, a heparin-conjugated affinity chromatography column, or any combination thereof. 8. The method of claim 1, wherein the sample is contacted with the heparin coated solid support at 4° C. for at least 30 minutes. 9. The method of claim 1, wherein the solid support-bound EV is separated from the sample by centrifugation, elution, or magnetization. 10. The method of claim 1, wherein the solid support-bound EV is released from the solid support by incubating the solid support-bound EV with salt. 11. The method of claim 1, wherein the solid support-bound EV is released from the solid support by incubating the solid support-bound EV with 2 M sodium chloride. 12. The method of claim 10, wherein the incubating is performed at 4° C. for at least 30 minutes. 13. The method of claim 1, wherein the solid support-bound EV is released from the solid support by incubating the solid support-bound EV with heparinase. 14. A kit for use in performing the method of claim 1. 15. The method of claim 1, the method further comprising loading the isolated EV with a therapeutic agent. 16. The method of claim 15, wherein the therapeutic agent is a siRNA, a miRNA, an antisense oligonucleotide, a polypeptide, a viral vector, or a drug.
※ AI-Helper는 부적절한 답변을 할 수 있습니다.