초록 ▼

A method and kit for estimating the amount of methyl anthranilate includes obtaining a sample suspected of having methyl anthranilate, acidifying the sample with a strong acid, adding sodium nitrite to the acidified sample producing nitrous acid which in the presence of methyl anthranilate produces

A method and kit for estimating the amount of methyl anthranilate includes obtaining a sample suspected of having methyl anthranilate, acidifying the sample with a strong acid, adding sodium nitrite to the acidified sample producing nitrous acid which in the presence of methyl anthranilate produces a diazonium ion. The method further comprises a step of adding sulfamic acid which removes excess nitrous acid from the sample, followed by the addition N-(1-naphthyl) ethylenediamine which reacts with the diazonium ion to form a colored complex which is color responsive which is then compared to standards prepared using known amounts of methyl anthranilate. The kit provides the a sample holder and vials of ingredients and standards to practice the method.

대표청구항 ▼

A method for estimating the amount of methyl anthranilate (MA) in water, comprising the steps of: a) obtaining a sample suspected of having methyl anthranilate (MA); b) acidifying the sample with a strong acid; c) adding sodium nitrite to the acidified sample producing nitrous acid which in the pres

A method for estimating the amount of methyl anthranilate (MA) in water, comprising the steps of: a) obtaining a sample suspected of having methyl anthranilate (MA); b) acidifying the sample with a strong acid; c) adding sodium nitrite to the acidified sample producing nitrous acid which in the presence of methyl anthranilate produces a diazonium ion; d) adding a reagent to remove excess nitrous acid; e) adding N-(1-naphtyl)ethylenediamine which reacts with the diazonium ion to produce a measurable color response; and f) comparing the obtained color response to standards prepared using known amounts of methyl anthranilate to estimate the amount of methyl anthranilate in said sample.