초록

Protein-free cell culture media supplements are described consisting of synergistic combinations of medium components, which when added to cell culture media, either serum supplemented or serum-free, enhance cell growth, culture longevity and product expression.

대표청구항 ▼

[ We claim:] [1.] A method of growing cells, comprising the step of contacting said cells with a cell culture medium comprising the following components:(a) a first reagent selected from the group consisting of glutamine, glutamate, and asparagine, said first reagent being present at a concentration

[ We claim:] [1.] A method of growing cells, comprising the step of contacting said cells with a cell culture medium comprising the following components:(a) a first reagent selected from the group consisting of glutamine, glutamate, and asparagine, said first reagent being present at a concentration of at least 8 mM;(b) phospholipid precursors comprising at least choline and ethanolamine;(c) tryptophan; and(d) an amino acid other than tryptophan and other than said first reagent of (a); wherein said components are at concentrations effective to increase culture longevity and product expression by maintaining said cells in a pseudo-stationary growth phase for about 100 hours or longer.