초록

Chemically defined media compositions for the culture of eukaryotic cells are disclosed. The compositions are useful for eukaryotic cell culture in perfusion bioreactors and other vessels.

대표청구항 ▼

The invention claimed is: 1. A soluble composition, suitable for producing a final volume of cell culture media, wherein the composition comprises the following components in the following amounts per liter of the final volume of cell culture media: anhydrous CaCl2, 5-200 mg; anhydrous MgCl2, 15-50

The invention claimed is: 1. A soluble composition, suitable for producing a final volume of cell culture media, wherein the composition comprises the following components in the following amounts per liter of the final volume of cell culture media: anhydrous CaCl2, 5-200 mg; anhydrous MgCl2, 15-50 mg; anhydrous MgSO4, 20-80 mg; FeSO4.7H2O, 0.05-0.50 mg; Fe(NO3)3.9H2O, 0.01-0.08 mg; ZnSO4.7H2O, 0.40-1.20 mg; ferric ammonium citrate, 0.04-200 mg; KCl, 280-500 mg; NaCl, 5000-7500 mg; NaH2PO4.H2O, 30-100 mg; Na2HPO4, 30-100 mg; CuSO4.5H2O, 0.001-0.005 mg; CoCl2.6H2O, 0.001-0.10 mg; (NH4)6Mo7O24 4H2O, 0.001-0.005 mg; MnSO4.H2O, 0.000070-0.0080 mg; NiSO4.6H2O, 0.000025-0.0005 mg; Na2SeO3, 0.004-0.07 mg; Na2SiO3.9H2O, 0.02-0.4 mg; SnCl2.2H2O, 0.000025-0.0005 mg; NH4VO3, 0.0001-0.0025 mg; D-Glucose, 500-8000 mg; sodium pyruvate, 0.0-1000 mg; sodium hypoxanthine, 0.0-20.0 mg; glycine, 0.0-150 mg; L-alanine, 0.0-150 mg; L-arginine.HCl, 200-5000 mg; L-asparagine.H2O, 40-250 mg; L-aspartic acid, 20-1000 mg; L-cysteine.HCl H2O, 25.0-250 mg; L-cystine.2HCl, 15-150 mg; L-glutamic acid, 0-1000 mg; L-histidine.HCl.H2O, 100-500 mg; L-isoleucine, 50-1000 mg; L-leucine, 50-1000 mg; L-lysine.HCl, 100-1000 mg; L-methionine, 50-500 mg; L-ornithine.HCl, 0-100 mg; L-phenylalanine, 25-1000 mg; L-proline, 0-1000 mg; L-serine, 50-500 mg; L-taurine, 0-1000 mg; L-threonine, 50-600 mg; L-tryptophan, 2-500 mg; L-tyrosine.2Na.2H2O, 25-250 mg; L-valine, 100-1000 mg; d-biotin, 0.04-1.0 mg; D-calcium pantothenate, 0.1-5.0 mg; choline chloride, 1-100 mg; folic acid, 1-10 mg; i-Inositol, 10-1000 mg; nicotinamide, 0.5-30 mg; p-aminobenzoic acid, 0.1-20 mg; riboflavin, 0.05-5.0 mg; thiamine.HCl, 0.5-20 mg; thymidine, 0-3.0 mg; vitamin B12, 0.05-5.0 mg; linoleic acid, 0.01-2.0 mg; DL-α-lipoic acid, 0.03-1.0 mg; pyridoxine.HCl, 0.5-30 mg; putrescine.2HCl, 0.025-0.25 mg; and ethanolamine.HCl, 2-100 mg. 2. The soluble composition of claim 1 further comprising a buffering molecule with a pKa between 5.9 and 7.8 and a cell protectant. 3. The soluble composition of claim 2 wherein the buffering molecule consists of MOPS in the amount of 1047-5230 mg per liter of final media volume and the cell protectant consists of Pluronic-F68 in the amount of 250-1500 mg per liter of final media volume. 4. A composition comprising a cell culture media made by the steps comprising: a) selecting a final media volume; b) providing the soluble composition of claim 2 or claim 3; c) solubilizing the soluble composition in a volume of water less than the final media volume; d) adding 1.022 g of L-glutamine per liter of final media volume; e) adding a bicarbonate ion providing substance sufficient to produce a bicarbonate ion concentration of between 0.020 M and 0.030 M in the final media volume; f) optionally adding at least one substance selected from the group consisting of mycophenolic acid, hypoxanthine, xanthine, and soy hydrolysate; g) adding a quantity of base sufficient to adjust the pH of the solution to between pH 5.9 and pH 7.8; and h) adding water sufficient to bring the volume of the composition to the selected final media volume. 5. The composition of claim 4 where the bicarbonate ion providing substance sufficient to a produce a bicarbonate ion concentration of between 0.020 M and 0.030 M in the final media volume is 2.1 g of NaHCO3 per liter of final media volume. 6. A soluble composition, suitable for producing a final volume of cell culture media, wherein the composition comprises the following components in the following amounts per liter of the final volume of cell culture media: CaCl2, 100.95 mg; MgCl2, 24.77 mg; MgSO4, 42.24 mg; FeSO4.7H2O, 0.3607 mg; Fe(NO3)3.9H2O, 0.0432 mg; ZnSO4.7H2O, 0.6225 mg; ferric ammonium citrate, 43.25 mg; KCl, 386.9 mg; NaCl, 5866.0 mg; NaH2PO4.H2O, 54.07 mg; Na2HPO4, 61.44 mg; CuSO4.5H2O, 0.003287 mg; CoCl2.6H2O, 0.0020606 mg; (NH4)6Mo7O24.4H2 O, 0.000535 mg; MnSO4.H2O, 0.00008571 mg; NiSO4.6H2O, 0.0000514 mg; Na2SeO3, 0.007489 mg; Na2SiO3.9H2O, 0.03671 mg; SnCl2.2H2O, 0.0000488 mg; NH4VO3, 0.0002530 mg; D-Glucose, 3680.52 mg; sodium pyruvate, 100 mg; sodium hypoxanthine, 2.069 mg; glycine, 16.23 mg; L-alanine, 79.31 mg; L-arginine.HCl, 674.89 mg; L-asparagine.H2O, 182.25 mg; L-aspartic acid, 67.23 mg; L-cysteine.HCl.H2O, 57.63 mg; L-cystine.2HCl, 106.70 mg; L-glutamic acid, 6.36 mg; L-histidine.HCl.H2O, 250.55 mg; L-isoleucine, 245.43 mg; L-leucine, 263.42 mg; L-lysine.HCl, 276.41 mg; L-methionine, 85.40 mg; L-ornithine.HCl, 2.44 mg; L-phenylalanine, 104.23 mg; L-proline, 14.94 mg; L-serine, 146.36 mg; L-taurine, 3.64 mg; L-threonine, 199.09 mg; L-tryptophan, 70.71 mg; L-tyrosine.2Na.2H2O, 195.58 mg; L-valine, 174.34 mg; d-biotin, 0.4359 mg; D-calcium pantothenate, 1.9394 mg; choline chloride, 10.8009 mg; folic acid, 3.4329 mg; i-inositol, 81.7965 mg; nicotinamide, 3.1342 mg; p-aminobenzoic acid, 2.1645 mg; riboflavin, 0.5359 mg; thiamine.HCl, 2.3377 mg; thymidine, 0.316 mg; vitamin B12, 0.5887 mg; linoleic acid, 0.0364 mg; DL-α-lipoic acid, 0.0909 mg; pyridoxine.HCl, 3.0442 mg; putrescine.2HCl, 0.0701 mg; and ethanolamine.HCl, 14.37 mg. 7. The soluble composition of claim 6 further comprising a buffering molecule with a pKa of between 5.9 and 7.8 and a cell protectant. 8. The soluble composition of claim 7 wherein the buffering molecule consists of MOPS in the amount of 2709.66 mg per liter of final media volume, and the cell protectant consists of Pluronic-F68 in the amount of 865.80 mg per liter of final media volume. 9. The soluble composition of claim 7 further comprising the following components in the following amounts per liter of final media volume: 0.5 mg mycophenolic acid; 2.5 mg hypoxanthine; and 50 mg xanthine. 10. A composition comprising a cell culture media made by the steps comprising: a) selecting a final media volume; b) providing the soluble composition of claim 7 or claim 8; c) solubilizing the soluble composition in a volume of water less than the final media volume; d) adding 1.022 g of L-glutamine per liter of final media volume; e) adding a bicarbonate ion providing substance sufficient to produce a bicarbonate ion concentration of between 0.020 M and 0.030 M in the final media volume; f) optionally adding at least one substance selected from the group consisting of mycophenolic acid, hypoxanthine, xanthine and soy hydrolysate; g) adding a quantity of base sufficient to adjust the pH of the solution to between pH 5.9 and pH 7.8; and h) adding water sufficient to bring the volume of the composition to the selected final media volume. 11. The composition of claim 10 where the bicarbonate ion providing substance sufficient to a produce a bicarbonate ion concentration of between 0.020 M and 0.030 M in the final media volume is 2.1 g of NaHCO3 per liter of final media volume.