IPC분류정보
국가/구분 |
United States(US) Patent
등록
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국제특허분류(IPC7판) |
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출원번호 |
US-0573950
(2014-12-17)
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등록번호 |
US-9170198
(2015-10-27)
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발명자
/ 주소 |
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출원인 / 주소 |
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대리인 / 주소 |
Meyertons, Hood, Kivlin, Kowert & Goetzel, P.C.
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인용정보 |
피인용 횟수 :
0 인용 특허 :
1 |
초록
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Techniques are disclosed relating to analysis and reduction of crosstalk between signals. These techniques may be applicable in many fields, such as single-tube PCR or DNA melt analysis, PCR or melt data from neighboring wells of a multi-well plate, capillary electrophoresis data (e.g., DNA sequenci
Techniques are disclosed relating to analysis and reduction of crosstalk between signals. These techniques may be applicable in many fields, such as single-tube PCR or DNA melt analysis, PCR or melt data from neighboring wells of a multi-well plate, capillary electrophoresis data (e.g., DNA sequencing), gas chromatography, multispectral imaging, dual-color fluorescence correlation spectrometry, electrical crosstalk, etc. According to one embodiment, crosstalk between fluorescence signals from different species may be determined based on a correlation between the time derivatives of the fluorescence signals from the fluorescent species.
대표청구항
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1. A method, comprising: illuminating a sample with a light source, wherein the sample includes a plurality of fluorescent species, and wherein illuminating the sample with the light source excites at least two of the plurality of fluorescent species;measuring at least two fluorescence signals from
1. A method, comprising: illuminating a sample with a light source, wherein the sample includes a plurality of fluorescent species, and wherein illuminating the sample with the light source excites at least two of the plurality of fluorescent species;measuring at least two fluorescence signals from the sample, wherein the at least two fluorescence signals include component signals from the at least two of the plurality of fluorescent species; anddetermining, by an assaying device, an amount of crosstalk between the at least two fluorescence signals, wherein the determined amount of crosstalk is based on a correlation between respective time derivatives of the at least two fluorescence signals. 2. The method of claim 1, wherein determining the amount of crosstalk includes: estimating the amount of crosstalk based on an initial crosstalk matrix; anddetermining an adjusted crosstalk matrix by adjusting elements of the initial crosstalk matrix based on the correlation between the respective time derivatives of the at least two fluorescence signals. 3. The method of claim 2, wherein adjusting the elements includes iteratively adjusting the elements within defined ranges of corresponding values from the initial crosstalk matrix. 4. The method of claim 1, wherein the correlation between the respective time derivatives of the at least two fluorescence signals is based on a pointwise product of the respective time derivatives. 5. The method of claim 1, wherein an optimal crosstalk correction is determined by minimizing the correlation between the respective time derivatives of the at least two fluorescence signals. 6. The method of claim 1, further comprising outputting crosstalk-corrected fluorescence signals for the at least two of the plurality of fluorescent species. 7. The method of claim 1, wherein the sample is a multi-well sample. 8. The method of claim 7, wherein the at least two of the plurality of fluorescent species are disposed within corresponding at least two wells of the multi-well sample. 9. The method of claim 8, wherein the at least two of the plurality of fluorescent species are a single type of fluorescent species. 10. An apparatus, comprising: a light source configured to illuminate a sample including at least first and second fluorescent species;a detector configured to receive fluorescent light from the first and second fluorescent species in response to the illumination; anda processing unit configured to: receive first data indicative of the received fluorescent light from the first fluorescent species and second data indicative of the received fluorescent light from the second fluorescent species; anddetermine an amount of crosstalk between the first and second data, wherein the determined amount of crosstalk is based on a correlation between respective time derivatives of the first and second data. 11. The apparatus of claim 10, wherein the sample includes at least six fluorescent species. 12. The apparatus of claim 10, wherein the sample includes exactly two fluorescent species. 13. The apparatus of claim 10, wherein the light source is a broad-spectrum light source, and wherein the apparatus further includes a filter operable to restrict the illumination to a specified wavelength band. 14. The apparatus of claim 10, wherein the light source is a narrow-spectrum light source. 15. The apparatus of claim 10, wherein the sample is a single-tube PCR sample. 16. The apparatus of claim 10, wherein the sample is a multi-well PCR sample. 17. The apparatus of claim 16, wherein the first fluorescent species is disposed within a first well of the multi-well PCR sample, and wherein the second fluorescent species is disposed within a second well of the multi-well PCR sample. 18. The apparatus of claim 17, wherein the first fluorescent species and the second fluorescent species are a single type of fluorescent species. 19. A non-transitory computer-readable medium having instructions coded thereon that, when executed by a computing device, cause the computing device to carry out operations comprising: receiving data indicative of a plurality of time-varying signals, wherein the signals include crosstalk between at least first and second ones of the signals;determining respective time derivatives for the signals based on the data;determining an amount of correlation between the respective time derivatives;determining a crosstalk metric indicative of a level of crosstalk for the signals, wherein the crosstalk metric is based on the amount of correlation; anditeratively adjusting a plurality of crosstalk coefficients indicative of the crosstalk between the signals, wherein the adjusting is based on the crosstalk metric. 20. The medium of claim 19, wherein iteratively adjusting the plurality of crosstalk coefficients includes adjusting the crosstalk coefficients subject to a set of restrictions. 21. The medium of claim 20, wherein the set of restrictions includes preventing negative values for the crosstalk coefficients. 22. The medium of claim 19, wherein the amount of correlation is a pointwise product of the respective time derivatives. 23. The medium of claim 19, wherein the crosstalk metric is based on an integral of the amount of correlation.
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