Provided is a buffer composition capable of suppressing temperature dependency of the pH of a buffer solution, and a specimen analysis method and a specimen analysis system using the buffer composition, wherein the buffer composition contains a buffer substance A showing a positive correlation betwe
Provided is a buffer composition capable of suppressing temperature dependency of the pH of a buffer solution, and a specimen analysis method and a specimen analysis system using the buffer composition, wherein the buffer composition contains a buffer substance A showing a positive correlation between temperature and pH and a buffer substance B showing a negative correlation between temperature and pH.
대표청구항▼
1. A method of analyzing a specimen in a sample comprising subjecting the sample to high-performance liquid chromatography (HPLC), capillary electrophoresis (CE), or capillary electrochromatography using a buffer composition, wherein the buffer composition comprises: a buffer substance A showing a p
1. A method of analyzing a specimen in a sample comprising subjecting the sample to high-performance liquid chromatography (HPLC), capillary electrophoresis (CE), or capillary electrochromatography using a buffer composition, wherein the buffer composition comprises: a buffer substance A showing a positive correlation between temperature and pH;a buffer substance B showing a negative correlation between temperature and pH;a non-detergent amphoteric ionic substance; andan ionic pseudostationary phase,wherein: the buffer composition is a liquid having a pH in a range of 3.0 to 10.0,the pKa of the buffer substance A and the pKa of the buffer substance B are each within ±2.0 of the pH of the buffer composition,the buffer substance A is selected from the group consisting of citric acid, maleic acid, acetic acid, glutamic acid, malic acid, phthalic acid, oxalic acid, malonic acid, succinic acid, fumaric acid, and a salt thereof; an anime compound having a carboxy group; and a combination thereof,the buffer substance B is selected from the group consisting of an amine compound, phosphoric acid, boric acid, carbonic acid, phenol, and a salt thereof; an anime compound having a carboxy group; and a combination thereof and,the ionic pseudostationary phase is selected from the group consisting of chondroitin sulfate, heparin, fucoidan, and a salt thereof, and a combination thereof. 2. A specimen analysis system comprising the buffer composition as defined in claim 1. 3. The specimen analysis system according to claim 2, wherein a temperature controller is not present. 4. The method according to claim 1, wherein the non-detergent amphoteric ionic substance is selected from the group consisting of a non-detergent sulfobetaine, a non-detergent carboxy betaine, and a combination thereof. 5. The method according to claim 1, wherein the non-detergent amphoteric ionic substance is a non-detergent sulfobetaine. 6. The method according to claim 1, wherein the buffer substance A is selected from the group consisting of citric acid, maleic acid, malic acid, phthalic acid, oxalic acid, malonic acid, succinic acid, fumaric acid, and a salt thereof; glutamic acid; and a combination thereof. 7. The method according to claim 1, wherein the buffer substance A is selected from the group consisting of citric acid, maleic acid, and a salt thereof; glutamic acid; and a combination thereof. 8. The method according to claim 1, wherein the buffer substance B is the amine compound or the amine compound having a carboxy group. 9. The method according to claim 8 wherein the amine compound is selected from the group of piperazine, pyridine, imidazole, 2-morpholinoethanesulfonic acid (MES), 3-morpholinopropanesulfonic acid (MOPS), piperazine-N,N′-bis(2-ethanesulfonic acid)(PIPES), 2-[4-(2-hydroxyethyl)-1-piperazinyl]ethanesulfonic acid (HEPES), 2-hydroxy-3-[4-(2-hydroxyethyl)-1-piperazinyl]propanesulfonic acid (HEPPSO), and 3-[4-(2-hydroxyethyl)-1-piperazinyl]propanesulfonic acid (EPPS). 10. The method according to claim 8, wherein the amine compound having a carboxy group is selected from the group of N,N-bis(2-hydroxyethyl)glycine (Bicine), N-[tris(hydroxymethyl)methyl]glycine (Tricine), N-(2-acetamide)iminodiacetic acid (ADA), glutamine, glutamic acid, histidine, threonine, serine, arginine, glycine, alanine, β-alanine, α-aminobutyric acid, β-aminobutyric acid, valine, cysteine, methionine, asparagine, aspartic acid, proline, hydroxyproline, leucine, isoleucine, tyrosine, phenylalanine, ornithine, lysine, and tryptophan. 11. The method according to claim 1, wherein the buffer substance B is selected from the group consisting of PIPES (piperazine-1,4-bis(2-ethanesulfonic acid), piperazine, histidine, and a combination thereof. 12. The method according to claim 1, wherein the buffer composition comprises any one of the following combinations: maleic acid and PIPES (piperazine-1,4-bis(2-ethanesulfonic acid); glutamic acid and piperazine; citric acid and histidine; and citric acid and piperazine. 13. The method according to claim 1, wherein the pKa of the buffer substance A and the pKa of the buffer substance B are within ±1.5 of the pH of the buffer composition. 14. The method according to claim 1, wherein the pKa of the buffer substance A and the pKa of the buffer substance B are within ±1.0 of a pH of the buffer composition. 15. The method according to claim 1, wherein the buffer composition has a pH in a range of 4.0 to 8.0. 16. The method according to claim 1 further comprising introducing the buffer composition into a capillary channel. 17. The method according to claim 1, wherein the method does not use a temperature controller. 18. The method according to claim 1 further comprising preparing the sample with the buffer composition.
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이 특허에 인용된 특허 (3)
Magers Thomas A. (South Bend IN), Composition and method of assaying for ketone bodies.
Chevign Roland (Wepion BEX) Janssen Jacques (Brussels BEX), Method for the separation of glycosylated hemoglobin Hb A1c by agarose gel electrophoresis.
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