초록 ▼

The present invention relates to a slide chip for a sensor for detection of food-borne bacteria and a fabrication method thereof. More particularly, the invention relates to a slide chip for a sensor for detection of food-borne bacteria and a fabrication method thereof, the slide chip comprising: a

The present invention relates to a slide chip for a sensor for detection of food-borne bacteria and a fabrication method thereof. More particularly, the invention relates to a slide chip for a sensor for detection of food-borne bacteria and a fabrication method thereof, the slide chip comprising: a substrate coated with a metal; a linker having a substituent which may be bonded to the metal and is located at the 5′ end of deoxythymidine (dT); and a food-borne bacterium-derived RNA aptamer that is bound to the linker by the 3′-end poly A tail. The slide chip makes it possible to detect food-borne bacteria in a rapid and accurate manner.

대표청구항 ▼

1. A slide glass for an optical microscope for detection of food-borne bacteria comprising: a glass substrate nano-coated with a metal;a linker having a substituent bonded to the metal and located at the 5′ end of deoxythymidine (dT); andan RNA aptamer bonded to the linker by the 3′-end poly A tail,

1. A slide glass for an optical microscope for detection of food-borne bacteria comprising: a glass substrate nano-coated with a metal;a linker having a substituent bonded to the metal and located at the 5′ end of deoxythymidine (dT); andan RNA aptamer bonded to the linker by the 3′-end poly A tail,wherein the RNA aptamer is an aptamer binding to lipopolysaccharide of E. coli O157:H7, an aptamer binding to ompC protein of Salmonella typhimurium strain, or an aptamer binding to teichoic acid of Staphylococcus aureus. 2. The slide glass of claim 1, wherein the RNA aptamer is obtained by substituting the 2′ carbon of RNA pyrimidine (cytosine and uracil) with fluorine. 3. The slide glass of claim 1, wherein the metal is silver or gold. 4. The slide glass of claim 1, wherein the substituent is a thiol group (—SH). 5. The slide chip of claim 1, wherein the slide glass further comprises a fluorescence tag having a 6-FAM molecule for fluorescence labeling of food-borne bacteria bonded to the RNA aptamer, the fluorescence tag is being located at the 5′ end of deoxythymidine (dT).