Compositions and methods for solid phase extraction of lipids
원문보기
IPC분류정보
국가/구분
United States(US) Patent
등록
국제특허분류(IPC7판)
G01N-001/34
B01J-020/02
B01J-020/06
B01J-020/10
B01J-020/32
G01N-030/60
G01N-030/46
출원번호
12785160
(2010-05-21)
등록번호
10434492
(2019-10-08)
발명자
/ 주소
Lu, Xiaoning
Shimelis, Olga I.
Ye, Maochun M.
출원인 / 주소
Sigma-Aldrich Co. LLC
대리인 / 주소
Frost, Kristin J.
인용정보
피인용 횟수 :
0인용 특허 :
0
초록▼
A composition, method and device for the preparation of biological samples for subsequent instrumental analyses, such as GC, GC-MS, LC and LC-MS analysis, using a solid phase extraction (SPE) process is described. Through SPE process alone or an integrated combination of protein precipitation, filtr
A composition, method and device for the preparation of biological samples for subsequent instrumental analyses, such as GC, GC-MS, LC and LC-MS analysis, using a solid phase extraction (SPE) process is described. Through SPE process alone or an integrated combination of protein precipitation, filtration, and SPE using a hydrophobic zirconia-coated chromatographic media, interfering compounds, such as proteins, glycerides and phosphate-containing compounds, are eliminated from the biological, food, environmental and biotechnology samples, affording an enhanced analyte response during the instrumental analysis.
대표청구항▼
1. A solid phase extraction (SPE) media for selective removal of lipids from a sample comprising: a. a porous silica substrate with any one or more of a particle size ranging from about 10 nm to about 1000 μm, a pore size ranging from about 30 Å to about 1000 Å, and a surface area ranging from about
1. A solid phase extraction (SPE) media for selective removal of lipids from a sample comprising: a. a porous silica substrate with any one or more of a particle size ranging from about 10 nm to about 1000 μm, a pore size ranging from about 30 Å to about 1000 Å, and a surface area ranging from about 5 m2/g to about 1000 m2/g;b. a hydrophobic alkyl linker having an attached end and a free end opposite the attached end, the attached end covalently bonded to the substrate, wherein the alkyl ranges from C3 to C18; and,c. zirconia bonded to the porous silica substrate through a bridging bond. 2. The SPE media of claim 1, wherein the sample comprises a biological sample, a food matrix, an environmental sample, a sample from a biotechnology process, or any combination thereof. 3. The SPE media of claim 1, wherein the sample further comprises an acidic analyte, a non-acidic analyte, or a mixture of an acidic analyte and a non-acidic analyte. 4. A solid phase extraction (SPE) media for selective removal of lipids from a sample comprising: a. a porous silica substrate with any one or more of a particle size ranging from about 10 nm to about 1000 μm, a pore size ranging from about 30 Å to about 1000 Å, and a surface area ranging from about 5 m2/g to about 1000 m2/g;b. a hydrophobic alkyl linker comprising an attached end covalently bonded to the substrate and a free end opposite the attached end, wherein the alkyl ranges from C3 to C18; and,c. zirconia covalently bonded through a bridging bond coupled to the free end of the hydrophobic alkyl linker. 5. The SPE media of claim 4, wherein the sample comprises a biological sample, a food matrix, an environmental sample, a sample from a biotechnology process, or any combination thereof. 6. The SPE media of claim 4, wherein the sample further comprises an acidic analyte, a non-acidic analyte, or a mixture of an acidic analyte and a non-acidic analyte. 7. A solid phase extraction (SPE) media for selective removal of lipids from a sample, the SPE comprising a mixture of: a. a first particle comprising zirconia bonded through a bridging bond to a porous silica substrate having any one or more of a particle size ranging from about 10 nm to about 1000 μm, a pore size ranging from about 30 Å to about 1000 Å, and a surface area ranging from about 5 m2/g to about 1000 m2/g,b. a second particle comprising a hydrophobic alkyl linker ranging from C3 to C18 having an attached end and a free end opposite the attached end, the attached end covalently bonded to a second porous silica substrate, the second porous silica substrate having any one or more of a particle size ranging from about 10 nm to about 1000 μm, a pore size ranging from about 30 Å to about 1000 Å, and a surface area ranging from about 5 m2/g to about 1000 m2/g. 8. The SPE media of claim 7, wherein the sample comprises a biological sample, a food matrix, an environmental sample, a sample from a biotechnology process, or any combination thereof. 9. The SPE media of claim 7, wherein the sample further comprises an acidic analyte, a non-acidic analyte, or a mixture of an acidic analyte and a non-acidic analyte.
※ AI-Helper는 부적절한 답변을 할 수 있습니다.