Method for preparing nicotinamide mononucleotide (NMN)
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IPC분류정보
국가/구분
United States(US) Patent
등록
국제특허분류(IPC7판)
C07H-019/048
C07H-019/207
C12N-009/10
C07H-001/04
C12P-019/30
출원번호
15574851
(2016-07-30)
등록번호
11040996
(2021-06-22)
국제출원번호
PCT/CN2016/092458
(2016-07-30)
국제공개번호
WO2017/185549
(2017-11-02)
발명자
/ 주소
Fu, Rongzhao
Zhang, Qi
출원인 / 주소
BONTAC BIO-ENGINEERING(SHENZHEN) CO., LTD
대리인 / 주소
Bayramoglu Law Offices LLC
인용정보
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초록▼
The present invention provides a method for preparing nicotinamide mononucleotide (NMN) by bioanalysis. The method includes a step of catalytically reacting a plurality of raw materials including nicotinamide, ATP, and ribose in the presence of nicotinamide phosphoribosyltransferase (Nampt), ribose
The present invention provides a method for preparing nicotinamide mononucleotide (NMN) by bioanalysis. The method includes a step of catalytically reacting a plurality of raw materials including nicotinamide, ATP, and ribose in the presence of nicotinamide phosphoribosyltransferase (Nampt), ribose phosphate pyrophosphokinase, and ribokinase, to prepare the NMN.
대표청구항▼
1. A method for preparing nicotinamide mononucleotide (NMN), comprising: catalytically reacting a plurality of raw materials comprising nicotinamide, ATP, and ribose in the presence of nicotinamide phosphoribosyltransferase (Nampt), ribose phosphate pyrophosphokinase, and ribokinase, to prepare the
1. A method for preparing nicotinamide mononucleotide (NMN), comprising: catalytically reacting a plurality of raw materials comprising nicotinamide, ATP, and ribose in the presence of nicotinamide phosphoribosyltransferase (Nampt), ribose phosphate pyrophosphokinase, and ribokinase, to prepare the NMN, wherein the Nampt is a protein derived from a protein having the amino acid sequence of SEQ ID NO: 2, wherein the amino acid sequence of the Nampt differs from the amino acid sequence of SEQ ID NO: 2 in that the Nampt has a mutation; wherein the mutation is selected from the group consisting of F180A, F180W, A182Y, E231A, E231Q, D298A, D298N, D298E, D338N, D338E, D377A, D377N, D377E, a combination of E231Q and D338E, a combination of E231Q and D377E, a combination of D338E and D377E, a combination of E231Q, D338E and D377E, and a combination of E231Q, D298A, D338E and D377E. 2. The method for preparing NMN according to claim 1, wherein the reaction takes place at a temperature of 30-50° C. and a pH of 6.5-8.5. 3. The method for preparing NMN according to claim 1, wherein the reaction takes place in the presence of Mg2+ and K+. 4. The method for preparing NMN according to claim 1, wherein the reaction takes place in Tris-HCl buffer. 5. The method for preparing NMN according to claim 1, wherein a concentration of the nicotinamide is 1-150 mM, a concentration of the ATP is 1-50 mM, and a concentration of the ribose is 1-100 mM. 6. The method for preparing NMN according to claim 1, wherein a molar ratio of the raw materials of nicotinamide, ATP, to ribose is 1-4:1:1-4. 7. The method for preparing NMN according to claim 6, wherein the molar ratio of the raw materials of nicotinamide, ATP, to ribose is 1.5:1:1.5.
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