초록

본 연구는 catecholamine 계토의 신경전달 물질의 합성에 관계하는 PMNT 효소의 유전자(cDNA)조작을 shotgun 방법에 의해서 그 염기서열을 알기 위하여 JM 107과 109의 숙주세포에 M13 mp 18과 19의 vector phage 에 유전공학적 방법으로 cloning 시키고 형질발현 실험을 통하여 숙주세포내에서 증폭되어진 template로서 순수분리 할 수 있었다. 순수분리된 template 는 Selective hybridization의 방법으로 검증한 결과 PMNT 유전자의 cDNA 임의 입증되었다.

Abstract ▼

This research was carried out to get template cloning for elucidating the base sequence of PMNT gene, which is concerened to make neurotransmitter enzyme. Copied DNA of PMNT was amplified in host bacteria and purified for cloning into vector phage(M13 mp 18 and 19 ). The DNA was treated by restrict

This research was carried out to get template cloning for elucidating the base sequence of PMNT gene, which is concerened to make neurotransmitter enzyme. Copied DNA of PMNT was amplified in host bacteria and purified for cloning into vector phage(M13 mp 18 and 19 ). The DNA was treated by restriction enzyme to make proper size for cloning. Cloned vector were introduced into host bacteria ( JM 107 and109 ) for amplification and purified for the materials of base sequencing of PMNT gene.

목차 Contents

• 1. 연구제목...6
• 2. 연구목적,내용 및 방법...6
• 2.1 연구목적...6
• 2.2 연구내용...6
• 2.3 연구방법...7
• 3. 주요연구내용...9
• 3. 1Plasmid의 증식 및 분리...9
• 3. 2CDNA의 분리 및 정제...9
• 3. 3CDNA의 Fragments형성...11
• 3. 4Temple 의 증식 및 분리...13
• 3. 5Gene cloning과 형질발현...14
• 3. 6Selective hybridization 시험...18
• 3. 7Ss template의 정제...18
• 4. 참고문헌...21
• 5. 논문발표실적 또는 계획...22