초록 ▼

지금까지 보고된 도라지에 관한 문헌을 수집하여 정리한 후 2년생 도라지의 약리성분과 생리적 활성을 토대로 22년생 도라지의 특수 성분은 분석하여 이들 물질의 약리작용을 in vivo 와 in vitro 상태에서 확인하였으며, 22년생 도라지에 많이 함유되어 있는 성분의 생리활성과 구조해석을 진행하여 건강보조식품 개발 에 적용함으로써 고소득 작물로 육성하고, 동시에 국민보건 향상에 기여한다.
다년생 도라지의 건강식품 소재화에 요구되는 주요 실험을
1. 4년생 및 다년생 도라지의 성분 분석 및 비교
2. 다년생도라

지금까지 보고된 도라지에 관한 문헌을 수집하여 정리한 후 2년생 도라지의 약리성분과 생리적 활성을 토대로 22년생 도라지의 특수 성분은 분석하여 이들 물질의 약리작용을 in vivo 와 in vitro 상태에서 확인하였으며, 22년생 도라지에 많이 함유되어 있는 성분의 생리활성과 구조해석을 진행하여 건강보조식품 개발 에 적용함으로써 고소득 작물로 육성하고, 동시에 국민보건 향상에 기여한다.
다년생 도라지의 건강식품 소재화에 요구되는 주요 실험을
1. 4년생 및 다년생 도라지의 성분 분석 및 비교
2. 다년생도라지의 유용물질 검색 및 활성 검사
3. 다년생도라지를 이용한 각종 건강 보조식품의 소재화로 대별하여 진행하였다.
2∼4년생과 22년생 도라지의 약리성분에 대한 검색 및 효능이 일차적으로 완료되 어 이들 실험 결과를 기초한 건강보조식품도 4종이 개발되었으므로, 또한 다년생 도라지 재배자 (77농가)를 중심으로 결성된 "성호 다년생 도라지 영농조합 법인" 에 기술을 이전하여 산지가공공장 설립을 하는데 있어 정부의 지원이 요망된다.

Abstract ▼

Platycodon radix is a dried root of Platycodon grandiflorum (P. grandiflorum) A. DC, a perennial grown on the hills and fields in Korea and Japan, or cultivated in various districts. The roots of P. grandiflorum have been used as an expectorant in the symptoms of cold, cough, asthma and pulmonary tu

Platycodon radix is a dried root of Platycodon grandiflorum (P. grandiflorum) A. DC, a perennial grown on the hills and fields in Korea and Japan, or cultivated in various districts. The roots of P. grandiflorum have been used as an expectorant in the symptoms of cold, cough, asthma and pulmonary tuberculosis and as valuable for the control of pleuritis in oriental medicine. As folk medicine, it is used in cough with sputum, tonsillitis, laryngeal pain, pulmonary pain, pertussis and inflammatary diseases.
Recently, P. grandiflorum was succeeded in cultivating for a long period above 22 years old and has generally been employed as folk remedy for diseases of adulthood such as hyperlipidemia, hypertension and diabetes.
Thus, this study was performed to ultimately develop the materials for health food using 22 years old P. grandiflorum by investigating effective medical compounds and examining their medical action in vitro and in vivo.
As the first step, the chemical compositions and effective compounds of 4 years old and 22 years old P. grandiflorum were analyzed and compared.
The contents of crude saponin, dietary fiber, inulin, inoraganic elements showed slight difference between 4 years and 22 years old P. grandiflorum.
Particularly, the significant difference of inulin oligomers from G-F/sub 2/ to G-F/sub 9/ in 4 and 22 years old roots suggested conformation change according cultivation period. Extracts of ethanol and methanol isolated from 4 and 22 years old roots were anlalyzed by HPLC. The results showed two different peaks which represented higher peak area in 22 years old root than that in 4 years old root.
To identify above compounds, various instrumental analyses were performed for petrelum, diethyl ether fractionation HPLC fraction, and the major compound was estimated to be steroid. This steroid had the free radical removal activity but did not show cell toxicity.
Ethanol and water extracts of 4 and 22 years old P. grandiflorum were tested for their antimicrobial activities against 15 types of strain. The ethanol extracts of 22 years old root inhibited the growth of all test organisms except Rhizopus javanicus. Minimal inhibition concentration against 15 types of strain, pH, temperature stability and extraction condition of antimicrobial agent were investigated.
To study the effect of P. grandiflorum(22 years old) feeding on serum and liver lipid concentration, diet-induced hyperlipidemic rats were fed diets containing 5% and 10% P. grandiflorum powder for 3 weeks. The P. grandiflorum feeding markedly decreased both serum and liver lipid concentrations in hyperlipidemic rats. Especially, 5% P. grandiflorum diet significantly decreased the concentration of total cholesterol and triglycerides in serum and liver as compared with those of the hyperlipidemic control group. Dietary P. grandiflorum also induced a reduction in low-density lipoprotein(LDL) cholesterol as well as an increase in the concentration of high-density lipoprotein (HDL) cholesterol in serum. Furtheremore, the atherogenic index was also low in rats fed P. grandiflorum diet. These results indicated that dietary P. grandiflorum may have a beneficial effect on preventing hypercholesterolemia and hyperlipidemia.
Six types of health food were developed using above results; enzyme treatment food, functional food and P. grandiflorum treated other medicinal herbs.

목차 Contents

• 제1장 서론...9
• 제2장 4년생 및 22년생 도라지의 성분분석 및 비교...13
• 제3장 다년생 도라지의 유용물질 검색...36
• 제4장 건강보조 식품의 개발...55
• 제5장 요약...59