초록 ▼

본 연구의 주요내용은 배로부터 생리활성물질의 분리 및 화학구조결정, 생리활성물질의 검증 및 고부가가치 제고를 위한 가공제품의 다양화 기술개발 등의 세 가지연구분야로 구성하였으며 각 연구분야별 연구개발 내용 및 범위는 다음과 같다.
1차 년도
제 1 세부과제 : 배의 생리활성물질의 분리 및 정제
1. 배 부산물의 아세톤 추출물의 분리
2. Thiolysis에 의한 thiolether유도체 형성 빛 정제
3. 탈유환원 반응
4. 저해물질의 부분정제
5. 흡광도 분포 측정
6. TLC에 의한 p

본 연구의 주요내용은 배로부터 생리활성물질의 분리 및 화학구조결정, 생리활성물질의 검증 및 고부가가치 제고를 위한 가공제품의 다양화 기술개발 등의 세 가지연구분야로 구성하였으며 각 연구분야별 연구개발 내용 및 범위는 다음과 같다.
1차 년도
제 1 세부과제 : 배의 생리활성물질의 분리 및 정제
1. 배 부산물의 아세톤 추출물의 분리
2. Thiolysis에 의한 thiolether유도체 형성 빛 정제
3. 탈유환원 반응
4. 저해물질의 부분정제
5. 흡광도 분포 측정
6. TLC에 의한 polypenol류 동정
7. 생리활성 물질의 분리 및 정제
1) Sephadex LH-20 에 의한 정제
2) MCI-gel CHP-20 에 의한 정제
3) Octadecyl silica gel 에 의한 정제
4) Toyopearl HW40 에 의한 정제
5) TLC에 의한 polyphenol류 동정
8. 효소저해 활성 측정
1) Glucosyltransferase
2) Tyrosinase
3) Xanthine oxidase
4) Angiotensin converting enzyme
제 2 세부과제(협동과제) : 배의 생리활성 불질의 면역기능
1, 동물실험
2. 시료투여 및 면역유발과정
3. 복강내 침출세포 조제
4. 비장 세포 조제
5. Thymus cell 부유액 조제
6. 채액성 면역시험
7, 세포성 면역시험
8. Macrophage계 세포활성능
9. 알러지 관련시험
1) 알러지 항원 조제
2) 혈중 histamine 측정
3) Mast cell로 부터 histamine 유리억제 활성 결정
제 3 세부과제(위탁과제) . 배의 착즙 전처리 조건 설정 및 배를 이용한 액상차의 개발
1. 배의 추출을 위한 전처리조건 검토
2. 착즙 전처리조건 및 추출방법 검토
3. 추출물의 농축조건 설정
4. 기호성 증진을 위한 적정 배합비 결정
5. 신선도 저하방지를 위한 액상차의 살균조건 검토
6. 배 액상차 제조공정 확립
2차 년도
제 4 세부과제 : 배의 저장기간에 따른 성분변화 및 배의 생리활성물질의 화학 구조 결정
1, 배의 저장기간에 따른 성분변화
1) 수분 및 pH
2) 유리당
3) 유기산
4) Polyphenol 함량
2. 생리활성 물질의 물리 및 화학적 성질
1) Melting point 측정
2) [α] a측정
3) Infrared spectrum에 의한 구조해석
4) Nuclear magnetic resonance(NMR)에 의한 구조해석
5) Mass spectrum에 의한 분자량 결정
6) 원소 분석
제 5 세부과제(협동과제) : 배의 생리활성물질의 항산화효과 및superoxide dismutase 활성 화
1. 세포배양계에서 생리활성 물질의 항산화 작용
1) 세포 배양간
2) 생리활성 물질의 항산화 작용
3) MIT Assay
2. 석유에테르 추출물 제조
3. Liposome제조
4. 지방산분석
5. Superoxide 유사활성물질 측정
제 6 세부과제(위탁과제) : 배와 생약재를 이용한 혼합 액상차의 개발 및생약재 적정 추출조건 설정
1. 배 액상차 제조시 필요한 부재료인 생약류의 선발
2. 국내 생산량이 많고 저렴한 생약류의 선발
3. 생약류 추출을 위한 전처리 조건 검토
4. 생약류 추출물 농축방법 검토
5. 생약재가 보강된 배 액상차의 개발
6. 배 액상차 제조공정 확립 및 배 액상차 제조
3차 년도
제 7 세부과제 : 배의 free radical 제거물질탐색 및 항암효과
1. Free radical scavenging 물질탐색
1) 7BARS 측정
2) Total iron 측정
3) free iron 측정
4) Ascorbic acid 함량측정
2. Oil emulsion과 deoxyribose에 의한 hydrexyl radical 생성물질 탐색(항암효과)
1) 전자공여능 반응 측정
2) H₂O₂ 함량측정
3. 리보솜 캐리어로서 항암효과
4. Sarcoma 180에 의한 항암실험
제 8 세부과제(협동과제) : 배의 생리활성물질의 지질대사에 미치는 영향
1, 지질 과산화 측정에 의한 항산화 효과
2. 혈중 및 가장에서 지질 분석
1) 총 지질의 분석
2) 총 콜레스테롤
3) Triglyceride 측정
4) HDL-콜레스테롤 측정
3. 분변에서의 지질 분석
1) 총 지질의 분석
2) 총 콜레스테롤
3) Triglyceride 측정
4) HDL-콜레스데롤 측정
제 9 세부과제(위탁과제) : 배를 이용한 타블렛 제품의 제조
1. 추출 및 농축조건 결정
2. 타블렛 제조 적정배합비 결정
3. 타블렛 제조를 위한 적정조건 검토
4. 타블렛 제품 제조공정 확립 및 타블렛 제품 제조

Abstract ▼

1) The isolation of biologically activated substances
The lyophilization of the solution extracted from 60 percent of acetone applied to pear, the compounding process in accordance with the solution's concentration, and the gel filteration through Sephadex G-50 of biologically activated compounds

1) The isolation of biologically activated substances
The lyophilization of the solution extracted from 60 percent of acetone applied to pear, the compounding process in accordance with the solution's concentration, and the gel filteration through Sephadex G-50 of biologically activated compounds obstructing enzyme action, such as glucosyltransferase, tyrosinase, xanthine oxidase, and angiotesin converting enzyme (ACE) led to the assumption that polyphenol was the compound serving as biologically activated substances obstructing enzyme action. Thus monosubstances were separated by the same means of polyphenol separation on the basis of that assumption.
Four kinds of compounds, compound A and B from F-1 fraction, compounds C from F-2 fraction, and compounds D from F-3 fraction were purified and re-crystallized after those compounds were fractionated as three fractions, F-1, F-f, and F-3.
It drew the deduction that judging from the degree of red color and definition of the compounds during the reaction period the separated compounds was tannin, a condensate, on the basis of the frame flavon-3-ol, having either of the chemical structures, one more hydroxyl added to compound B, or gallate added to the other parts. Finally these means brought an epoch-making solution to separating biologically activated substances from Korean pear
2) Immunofunction of biologically activited substances
This study was conducted to investigate immunofunctional activity of the polyphenol fractions isolated from pear. In the experimental of Rosette forming cell, the results showed that all the polyphenol fractions enhance the cell count compared with the control group. The phagocytic activity of peritoneal macrophage on mice was significantly increased by the polyphenol fractions of pear compared with that of the control group. Espectially polyphenol fraction Ⅱ and Ⅲ showed highly significant effect on Rosette forming cell, phagocytic activity and allergy inhibition.
Allergic contact dermatitis is a common skin disease resulting from specific immunologic sensitization due to topically applied allergens. Here the contact hypersensitivity was assayed and abdominal skin morphological changes including mast cells were examined. The number of mast cells was significantly decreased in the sample groups than the control group.
3) Establishment of pre-pressing condition on quality of pear juice and development of tea type product
Pears are one of the major fruits produced in Korea and favorably used for juice production. Browning is one of the major problems in the processing, storage and marketing of rear were examined in controlling the browning of pear juice during storage.
The objective of this study was to investigate the influence of several browning inhibitors on the color and free sugar Content of pear juice at different concentrations of the inhibitors with time during storage. Pear juice sample were treated with several browning inhibiters (ascorbic acid, citric acid, cysteine, sodium chloride and sodium bisulfite) at the concentrations of 0, 0.05, 0.1 and 0.2% (w/w), and stored at 4 and 38℃ The pH, total acidity, soluble solid content, extraction yield, turbidity, color change and the contents of free sugar (fructose sorbitol, glucose, sucrose) were measured at 0, 1, 3, 6, 10, 12, 15, 18 days of storage.
The brown color development of tear juice was inhibited by all browning inhibitors during storage at the concentrations of 0.05%, ascorbic acid, cysteine, sodium bisulfite were more effective in browning inhibition of pear juices than citric acid and sodium chloride. Ascorbic acid(0.05%) showed synergistic effect with cysteine(0.05%) and sodium bisulfite(0.01%) in browning inhibition. The content of fructose was higher than other free sugars in was little change in turbidity and total acidity during 18 days of storage. These results suggest that the selected browning inhibitors are effective in browning inhibition of pear juice while these are not changing the content of free sugars during storage.
4) Changes of pear during storage and chemical structure of biological activated compound from pear
The objective of this work was to seek alternative uses of Korean pear and improve its additional value. Polyphenols, free sugars, organic acids and their changes In Korean pear during storage were investigated. The antioxidant activity of polyphenols was evaluated. Polyphenols in different parts of pear, peel, pulp and core, reached 25.7%, pH decreased while the concentration of free sugars, including fructose, glucose and sucrose, all rose during storage. Pour organic acid, citric acid, citric acid and fumaric acid, were ditermined, and succinic acid was found to be the major organic acid in the purple, which decreased after storing for months. The antioxidative activity of polyphenol fraction Ⅰ, Ⅱ and m on cottonseed, linseed and fish oils were higher than that of the control group.
Xanthine oxidase involved in pruine metabolism oxidizes hypoxanthine to xanthine and xanthine to uric acid. In the continuous study for natural compound, nine flavan-3-ols have been isolated from the persimmon leaves. The structures of (+)-catechin, (+)-epigallocatechin, (-)-epigallocatechin and procyanidin B-7-3-O-gallate were established by NMR and their inhibitory effect on xanthine oxidase activity was investigated.
Procyanidin B-7-3-O-gallate and (-) -epigallocatechin showed 100%, 75.5% inhibition at 500 ppm and inhibited on the angiotensin converting enzyme respectively. Procyanidin B-7-3-0- gallate and (-)-epigallocatechin showed 80% inhibition at 500 ppm and inhibited on the xanthine oridase competitively, Procyanidin B-7-3-D-gallate and (-)-epigallocatechin showed 42.4%, 43.4% inhibition at 1000 ppm and Inhibited on the tyrosinase competitively.
5) Effect of antioxidation and superoxide dismutase biologically activated compounds
The lyophilization of the solution extracted from 60% of acetone from pear and fracted into fraction Ⅰ, Ⅱ and Ⅲ. The results showed slightly lower in polyphenol fraction Ⅱ and Ⅲ groups as compared with the control group. Electron donating ability of the polyphenol compounds by TBARS(thiobarbituric acid reactive substance) was the highest the polyphenol fraction Ⅲ.
The anti-oxidative activity of the polyphenol fraction Ⅰ, Ⅱ and Ⅲ on cottonseed, linseed and fish oils were higher than that of the control group. Electron donating abilities, which were measured from the fractions, showed above 80% in fraction Ⅱ, and Ⅲ at 50 ppm, and superoxide dismutase like activity recognized 50-60% effect in fraction Ⅱ and Ⅲ at 500 ppm.
Xanthin oxidase experiments in relation to antioxidant showed that inhibitor effect of fraction I Was very small, but the remaining fraction polyphenols were observed about 80% inhibitor effect.
6) Preparation of tea-type pear product and optimum extraction condition for herbs
Mixed tea-type pear products were made of three combination ratios that were used of cinnamon, ginger, jujube and various herbs. Combination ratio of cinnamon flavor product was cinnamon extract(10%), Angilica(10%), Hovenia dulcis(7.5%) and Astragalus mimbranaceus(7.5%), and that of jujube flavor product was jujube extract(20%), Ligusticum(5%), Pleuropterus maltflorus(5%) and Paeonia japonaca(5%). Ginger flavor product was composed of combination ratio for ginger extract(15%), Auranti Nobilis(10%), Platycidon grandiflorum(10%).
7) Investigation into biologically activated free radical of descavenging and effect of anticancer
TBARS values were measured from Ⅰ, Ⅱ and Ⅲ which were polyphenols extracted from pear. All fraction's TBARS values were higher than 0.1 of control value. TBARS values of polyphenols fraction Ⅰ, Ⅱ and Ⅲ were 0.4, 0.52 and 0.46 respectively. Among fractions, TBARS value of fraction H were 0.65, 0.66 under H₂O₂ · DH active oxygen and 0.47 under KO2 active oxygen. Because of the large effect of antioxidant, ascorbic acid including the rind of a pear was measured. The measured results skewed 3a, 19 and 16 ppm for fraction Ⅰ, Ⅱ and m respectively. For the elimination of radical, electronic donating abilities, which were measured, showde that dinating abilities of fraction Ⅰ very lower than of fraction Ⅱ and Ⅲ. However, fraction Ⅱ, Ⅲ recognized donating abilities of 52%, 59% at 50 ppn. Similar electronic donating abilities was identified at other concentration.
Antitumor effects of polyphenol fraction from pear were studied by using sarcoma 180. The body weight of the mice inplanted mice sarcoma 180 was slightly lower in polyphenol fraction Ⅰ, Ⅱ and Ⅲ groups as compared with the control group. The life prolongatin effects of the polyphenol fraction Ⅰ, Ⅱ and Ⅲ were 9.0%, 27.3% and 49.6% respectively when pulyphenol fraction Ⅰ, Ⅱ and Ⅲ were intraperitdneally injected to the mice. This results indicated that polyphenol fractions showed the strongest antitumor.
8) Effect of lipid metabolism on biologically activated Compounds
This study was conducted to investigate the effects of the polyphenol fraction isolated from pear on the reduction of fat accumulation in rats fed on hight fat diet for 5 weeks. It was to examine metabolism by analyzing biochemically the fat composition in serum, liver and feces. It was showed that the levels of total lipid and total cholesterol in serum was remarkably reduced in polyphenol fraction Ⅱ as compared with the control group. The liver was that the levels of total lipid and triglyceride was significantly lower than the control group.
The contents of total lipid, total cholesterol and triglyceride In feces were tended to be slightly increase polyphenol fraction compounds compared to control group. In the total protein and albumin, all experimental groups were lower compared to control group, which were not significant.
9) Preparation of pear tablet
The physiological activates of 11 kinds of herbs were examined. Hovenia dulcis extract showed highest value in nitrite-scavenging ability at pH 1.2, 91%. Tyrosinase inhibitory effects of jujube, Hovenia dulcis and Aurantii Nobilis extracts were 65%, 61% and 56%, respectively. Polyphenol contents of Platycondon, Aurantii Nobilis and Astragalus membramaceus extract were 61%, 54%, 50%, respectively. Electron donating ability of Paeonia japonica-t extract was the highest value, 80%. Pear huice was concentrated to about 65 "Brix under vacuum and mixed with glucose for preparing pew tablet.
2. Proposal for future application
The development and production of functional pear beverages through this study of high value added can contribute to the betterment of the national eating habits and the improvement of the national health. The technology acquired by this study can be used as part of the strategies for supporting farmers who have gone through financial difficulty by producing the pear tea of high value-added through the reusing process of waste resources as well as by extracting polyphenol, a compound isolated refired from persimmon leaves which is believed to keep blood pressure from going up, to be anti-cancer, and to increase immune-competence.
Technical experts can be trained by organically connecting groups of educational-industrial-research institutional professions. The significant information and technology acquired during the study period can be used as the database for the fields related to domestic pear Now that three you key study was final finished, nat only is the educational-industrial study of industrialization for massproduction supposed to begin but also it is supposed to initiate industrial organizations of pear tea beverages having biological effects mentioned earlier with the purpose of manufacturing profitable pear tea beverages.
The industrial property right is to be secured by applying for both patents on high function pen tablet beverages as well as brand-new functional food material.
Furthermore, much demand for to be made by actively publicizing the superiority of the biologically activated compounds of pear and by processing pear tablet as materials for medicine and food.

목차 Contents

• 표지...1
• 최종보고서...2
• 제출문...3
• 요약문...4
• SUMMARY...18
• CONTENTS...27
• 목차...36
• 제1장 연구개발 과제의 개요...47
• 제1절 연구개발의 목적...47
• 제2절 연구개발의 필요성...48
• 제3절 국내.외 관련기술의 현황과 문제점...49
• 제2장 국내.외 기술개발 현황...52
• 제3장 연구수행 내용 및 결과...57
• 제1절 배의 생리기능물질의 분리 및 정제...57
• 1. 실험 방법 및 연구내용...57
• 가. 배의 아세톤 추출물의 분리...57
• 나. Thiolysis에 의한 thiolether 유도체 형성 빛 정제...57
• 다. 탈류환원 반응...57
• 라. 흡광 분포도 측정...58
• 마. TLC에 의한 polyphenol류의 동정...58
• 바. 생리 활성물질의 분리 및 정제...58
• 1) Sephadex LH-20에 의한 정제...58
• 2) MCI-gel CHP 20P에 의한 정제...58
• 3) Octadecyl silica get(ODS)에 의한 분리...58
• 4) Toyoperal HW 40(TSK gel)...59
• 5) Bondapack C18 column에 의한 정제...59
• 사. 저해 물질의 부분정제...59
• 아. High performance liquid chromatography(HPLC)에 의한 polyphenol 화합물의 동정...59
• 자. 효소 저해활성 측정...59
• 1) Glucosyltransferase (Gtase)...59
• 2) Tyrosinase...60
• 3) Xanthine oxidase...60
• 4) Angiotensin converting enzyme(ACE)...61
• 2. 연구개발 결과...61
• 가. 배의 아세톤 추출물의 분리...61
• 나. 아세톤 추출물에 의한 효소저해 작용...61
• 다. 저해물질의 부분정제에 관한 수율...65
• 라. 부분정제에 의한 효소저해효과...66
• 마. Polyphenol 화합물의 분리 및 정제...70
• 3. 적 요...78
• 제2절 배의 생리활성물질의 면역기능...80
• 1. 실험방법 및 연구내용...80
• 가. 시료투여 및 면역 유발과정...80
• 나. 복강내 침출세포(Peritoneal exudative cells, PEC) 조제...80
• 다. 비장세포 (Spleen cells) 조제...81
• 라. Thymus cell 부유액 조제...81
• 마. 세포성 면역실험...81
• 1) Plague forming cell(PFC) 측정...81
• 2) 비장세포의 Rosette forming cell(RFC)의 검출...81
• 3) 항체 생산능 측정...82
• 바. Macrophage계 세포 활성능...82
• 1) 탐식능 측정...82
• 사. 알레르기 관련 실험...82
• 1) 알레르기 항원 조제...82
• 2) PCA(Passive cutaneous anaphylaxis) 실험...83
• 3) 혈중 histamine 측정...83
• 2. 연구개발의 결과...83
• 가. 배의 추출물의 용혈반 형성에 미치는 영향...83
• 나. 배의 추출물의 rosette 형성에 미치는 영향...86
• 다. 배의 추출물의 세포증식에 미치는 영향...88
• 라. 배의 추출물의 수동피부아나필락시스 저해 효과...90
• 마. 배의 추출물의 히스타민 억제 효과...93
• 3. 적 요...94
• 제3절 태의 착즙 전처리 조건설정 및 배를 이용한 액상차의 개발...95
• 1. 실험 방법 및 내용...95
• 가. 배의 착즙을 위한 전처리조건 검토...95
• 나. 배의 갈변 방지를 위한 첨가제 선정 시험...95
• 다. 첨가제 종류 및 저장기간에 따른 갈변 저해효과 비교시험...95
• 라. 배를 이용한 액상차의 개발...95
• 마. 배와 생약재를 이용한 혼합 액상차의 개발...96
• 바. 생약재 적정 추출조건 설정...96
• 사. 착즙수율...96
• 아. pH...96
• 자. 가용성 고형물...96
• 차. 탁도...97
• 카. 색도...97
• 타. Ascorbic acid...97
• 파. 유리당...97
• 하. 산도...98
• 갸. 아질산염 소거작용...98
• 냐. Tyrosinase 저해효과...99
• 댜. Polyphenol 함량...99
• 랴. 전자공여 작용...99
• 2. 연구개발 결과...100
• 가. 배의 착즙을 위한 전처리조건 검토...100
• 1) 착즙 수율...100
• 2) pH...101
• 3) 가용성 고형물...102
• 4) 탁 도...103
• 5) 색 도...104
• 나. 배의 갈변 방지를 위한 첨가제 선정 시험...106
• 1) Ascorbic acid 첨가...106
• 2) Cysteine 첨가...113
• 3) NaHSO₃ 첨가...121
• 다. 첨가제 종류 및 저장기간에 따른 갈변 저해효과...130
• 라. 배를 이용한 액상차의 개발...141
• 1) 음료제조...141
• 가) 농축조건 설정...141
• 나) 적정 배합비 선정...142
• 3. 적 요...145
• 제4절 배의 저장기간에 따른 성분변화 및 생리활성물질의 화학구조 결정...146
• 1. 실험 방법 및 연구내용...146
• 가. 배의 저장기간에 따른 성분변화...146
• 1) 수분함량 및 pH...146
• 2) 유리당...146
• 3) 유기산...146
• 4) polyphenol 함량...148
• 나. Polyphenol 화합물의 구조결정...149
• 1) Melting point 및 선광도 측정...149
• 2) Infrared Spectrum (IR) 측정...149
• 3) Nuclear magnetic resonance (NMR)에 의한 구조 해석...149
• 4) Mass spectrum에 의한 분자량 측정...149
• 다. 유도체 화합물의 형성 및 정제...150
• 1) Thiolysis 반응...150
• 2) 탈황반응...150
• 3) Tannase에 의한 가수분해 반응...150
• 2. 연구개발결과...153
• 가. 배의 저장에 따른 성분변화...153
• 1) 수분함량 및 pH 변화...153
• 2) 유리당 함량의 변화...153
• 3) 유기산 함량의 변화...154
• 4) Polyphenol 함량의 변화...154
• 나. Polyphenol 화합물의 구조분석...160
• 1) Compound A ( (+)-Catechin )...160
• 2) Compound B ( (+)-Gallocathin )...164
• 3) Compound C ( (-)-Epogallocathchin )...168
• 4) Compound D ( Procyanidin B-3-3-O-gallate )...172
• 다. 배의 polyphenol 화합물의 생리활성 검증...182
• 1) Angiotensin converting enzyme (ACE) 저해...182
• 2) Xanthine oxidase 저해...184
• 3) Tyrosinase 저해...186
• 3. 적 요...188
• 제5절 배의 생리활성물질의 항산화효과 및 superoxide dismutase 활성화...190
• 1. 실험방법 및 연구내용...190
• 가. 석유에테르 추출물 제조...190
• 나. 전자공여능에 의한 항산화 측정...190
• 다. SOD(superoxide dismutase)-유사활성 측정...190
• 라. 세포배양...191
• 마. 리보좀제조...191
• 바. 암세포 증식억제효과(MTT assay)...192
• 사. 지방산 분석...192
• 2. 연구개발의 결과...193
• 가. 석유에테르 추출물 제조...193
• 나. 항산화 효과...193
• 1) TBARS법에 의한 유지산화...193
• 2) 전자공여능에 미치는 영향...194
• 다. SOD (Superoxide dismutase)-유사활성 측정...194
• 라. 암세포 증식억제 효과 (MTT assay)...195
• 3. 적 요...205
• 제6절 배와 생약재를 이용한 혼합 액상차의 개발 및 생약재 적정 추출조건 설정...206
• 1. 배와 생약재를 이용한 혼합 액상차의 개발...206
• 가. 배 액상차 제조...206
• 1) 계피맛 배음료 제조...206
• 가) 부재료 선정 및 배합비 결정...206
• 2) 대추맛 배음료 제조...211
• 가) 부재료 선정 및 배합비 결정...211
• 3) 생강맛 배음료 제조...216
• 가) 부재료 선정 및 배합비 결정...216
• 2. 생약재 적정 추출조건 설정...220
• 가. 생약재의 적정 추출조건 설정...220
• 나. 열수 추출...221
• 다. 50% 에탄올 추출...222
• 라. 75% 에탄올 추출...223
• 마. 산 도...224
• 바. 탁 도...225
• 사. 색 도...228
• 아. 유리당...232
• 3. 생약재의 기능성 성분 탐색...233
• 가. 아질산염 소거작용...233
• 나. Tyrosinase(Polyphenol oxidase, PPO) 저해작용...236
• 다. 폴리페놀 함량...237
• 라. 전자공여 작용...238
• 4. 적 요...240
• 제7절 배의 free radical scavenging물질 탐색 및 항암효과...241
• 1. 실험방법 및 연구내용...241
• 가. Free radical scavenging 물질탐색...241
• 1) Thiobarbituric acid reactive substances (TBARS) 분석...241
• 2) 비햄철(Nonheme iron) 측정...241
• 3) Ascorbic acid 측정...242
• 나. Oil emulsion과 deoxyribose에 의한 hydroxyl radical 생성물질 탐색...242
• 1) Oil emulsion 제조...242
• 2) Polyphenol oil emulsion 시료제조...242
• 3) 전자공여능 측정...242
• 다. 리보좀 캐리어로서 Sarcoma 180에 의한 항암효과...243
• 1) Sarcoma 180 세포 배양...243
• 2) Sarcoma 180의 복수암 유발...243
• 3) 시료 투여...243
• 4) 체중증가량 측정...243
• 2. 연구개발의 결과...244
• 가. Free radical scaveng 물질 탐색...244
• 1) 비햄철 (Nonheme iron) 측정...244
• 2) Ascorbic acid 측정...245
• 나. Oil emulsion에 따른 hydroxyl radical 생성물질탐색...246
• 1) Oil emulsion 제조...246
• 2) 전자공여능 측정...247
• 3) TBARS 에 의한 H2O2 함량 측정...248
• 다. 암세포 증식에 미치는 영향...250
• 라. Sarcoma 180에 의한 항암 효과...250
• 3. 적 요...256
• 제8절 배의 생리활성물질의 지질대사에 미치는 영향...257
• 1. 실험방법 및 연구내용...257
• 가. 실험동물 및 식이...257
• 나. 혈장, 간장, 지방조직 및 분변 채취...257
• 다. 혈장중의 지질분석...259
• 1) 총 지질의 분석...259
• 2) 총 콜레스테롤의 분석...259
• 3) Triglyceride (중성지질) 측정...259
• 4) HDL-콜레스테롤 측정...259
• 5) LDL-콜레 스테롤 측정...260
• 라. 총 단백질 및 알부민 정량...260
• 마. 간장 및 분변 중에서 각종 지질 정량...260
• 바. 유의성 검정...260
• 2. 연구개발의 결과...260
• 가. 총 지질 분석...260
• 1) 혈청, 간장 및 분변 중의 지질분석...260
• 나. 총 콜레스테롤 함량...261
• 다. 중성지질 함량 측정...261
• 라. HDL-cholesterol 함량 측정...262
• 마. LDL-cholesterol 함량 측정...262
• 바. 혈청 중 총 단백질과 알부민 함량 측정...263
• 3. 적 요...276
• 제9절 배를 이용한 타블렛 제품의 제조...277
• 1. 배 추출 및 농축조건 설정...277
• 2. 타블렛 제조 적정 배합비 결정...277
• 3. 타블렛 제조공정...277
• 4. 배 타블렛 제품의 개발...278
• 5. 적 요...282
• 제4장 목표달성도 및 관련분야에서의 기여도...283
• 제1절 목표달성도...283
• 제2절 관련분야 기여도...286
• 제5장 연구개발 결과의 활용 계획...288
• 제6장 연구개발 과정에서 수집한 해외 과학 기술 정보...290
• 제7장 참고 문헌...294