초록 ▼

- 주당 (jud) 유전자 4개의 genetic mapping 완료
- 2개의 유전자 cosmid rescue, 1개의 유전자 cloning 완료
- class Ⅰ 유전자 4개, class Ⅱ 유전자 2개, class Ⅲ 유전자 3개, class Ⅳ 유전자 2개 발현 양상 분석 완료
- intestine-enriched mount 8에 속하는 유전자 7개의 발현 확인 완료
- 변이 스크리닝 조건 확립
- 약 40,000 염색체 검색, 94개의 후보 변이 확보
- 시간대 별 2D gel 완료
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- 주당 (jud) 유전자 4개의 genetic mapping 완료
- 2개의 유전자 cosmid rescue, 1개의 유전자 cloning 완료
- class Ⅰ 유전자 4개, class Ⅱ 유전자 2개, class Ⅲ 유전자 3개, class Ⅳ 유전자 2개 발현 양상 분석 완료
- intestine-enriched mount 8에 속하는 유전자 7개의 발현 확인 완료
- 변이 스크리닝 조건 확립
- 약 40,000 염색체 검색, 94개의 후보 변이 확보
- 시간대 별 2D gel 완료
- 10개의 차별적 발현 단백질 동정 완료
- 유전자 34개의 RNAi 표현형질을 확인함
- 유전자 간의 상호 작용 및 유전학적 작용 경로에 대한 가설 설정
- class 별 유전자의 expression mapping 및 발현 조절 조사 및 ethanol response element의 발견
- 동정된 단백질의 클로닝, 유전자 구조 결정 및 발현 분석 수행
- 초기에 phosphorylation의 변화를 보이는 ina-1 유전자의 발견

Abstract ▼

Ⅰ. The nematode shows responses to acute ethanol exposure that are similar to those observed in humans, mice, and Drosophila, namely hyperactivity followed by uncoordination and sedation. We used in this report the nematode C. elegans as a model system to identify and characterize the genes that are

Ⅰ. The nematode shows responses to acute ethanol exposure that are similar to those observed in humans, mice, and Drosophila, namely hyperactivity followed by uncoordination and sedation. We used in this report the nematode C. elegans as a model system to identify and characterize the genes that are affected by ethanol exposure and to functionally link those genes into an ethanol-induced gene network.By analyzing the expression profiles of the entire C. elegans ORFs using microarrays, we identified genes affected by ethanol and classified them into five groups. By mapping each gene on the expression mountain map, we established a global expression profile of the response to ethanol stress at the organismic level in the nematode. We further characterized the genes in class I, which may represent the genes directly involved in the ethanol response. Class I genes included many heat shock protein genes, indicating that high concentration of ethanol acts as a strong stress to the animal. Interestingly, we identified two non-heat shock protein genes that were specifically responsive to ethanol. glr-2 was the only glutamate receptor gene to be induced by ethanol. T28C12.4, which encodes a protein with limited homology to human neuroligin, was also specific to ethanol stress. Finally, by analyzing the promoterregions of the class I genes both in C. elegans and C. briggsae, we identified a regulatory element, TCTGCGTCTCT, that was necessary for the expression of subsets of ethanol response genes.
Ⅱ. Small heat shock proteins are induced by various stresses. We here report the orientation-dependent hypoxia response of the promoters of the hsp-16 genes in the nematode. The hsp-16.1 and hsp-16.2 genes in C. elegans responded to hypoxia, while hsp-16.41 and hsp-16.48, which share the promoter regions with hsp-16.1 and hsp-16.2 respectively, did not. For comparative genomic analysis, we identified 10 hsp-16 genes in the nematode Caenorhabditis briggsae from the genome database.The comparison of the promoter sequences revealed a new conserved sequence block, CAC(A/T)CT, that was required for the orientation-dependent hypoxia response, but not for other stress responses such as heat or ethanol. We propose a working model for the orientation-dependent promoter usage between two genes sharing the promoter region. We also discuss a possible application of the hypoxia-inducible promoter for conditional gene expression.

목차 Contents

• 표지...1
• 제출문...2
• 보고서 초록...3
• 요약문...4
• SUMMARY...10
• 목차...11
• 제 1 장 연구개발과제의 개요...12
• 제 2 장 국내외 기술개발 현황...13
• 제 3 장 연구개발수행 내용 및 결과...15
• 제 4 장 목표달성도 및 관련분야에의 기여도...36
• 제 5 장 연구개발결과의 활용계획...39
• 제 6 장 참고문헌...39