산화적 스트레스를 생성은 암, 치매, 동맥경화의 발생과 진행에 관여하며, 세포내의 산화환원 평형의 불균형을 질병의 발생을 촉진시키거나 여러 신호전달 경로를 활성화시켜 관련된 많은 만성질환을 촉발함. 따라서 항산화 활성뿐만 아니라 관련 신호전달 경로에 대한 저해활성이 뛰어난 식품성분이 좋은 질병 예방 기능성 소재로 활용될 수 있음. 산화적 스트레스에 의한 암화과정 유도기작 규명, 베타아밀로이드, 과산화수소, peroxynitrite 등에 의한 뇌신경세포 사멸 기작 규명, thrombin, platelet-derived growth f
산화적 스트레스를 생성은 암, 치매, 동맥경화의 발생과 진행에 관여하며, 세포내의 산화환원 평형의 불균형을 질병의 발생을 촉진시키거나 여러 신호전달 경로를 활성화시켜 관련된 많은 만성질환을 촉발함. 따라서 항산화 활성뿐만 아니라 관련 신호전달 경로에 대한 저해활성이 뛰어난 식품성분이 좋은 질병 예방 기능성 소재로 활용될 수 있음. 산화적 스트레스에 의한 암화과정 유도기작 규명, 베타아밀로이드, 과산화수소, peroxynitrite 등에 의한 뇌신경세포 사멸 기작 규명, thrombin, platelet-derived growth factor 인간대동맥평활근 세포의 동맥경화 유도 분자 기작 규명을 하였음. 전통 식품인 재료인 자두, 사과, 소나무 잎 추출물 등과 식품 내 함유된 다양한 성분인 vitamin C, curcumin, peonidin, piceatannol, resveratrol, procyanindin등의 주요 항산화 성분을 이용하여 이들의 질병 예방 효능을 측정하였음. 또한, 이들 성분들이 세포내 신호전달 관련 단백질들 및 전사인자의 활성화를 조절함으로써 암, 뇌신경 세포 사멸, 동맥경화에 대한 저해 활성을 가지는 것을 규명하였음. 본 연구 결과를 통해 SCI급 전문학술지에 17편 발표하였으며, 이를 국제학술대회에서 발표하여 전통식품의 세계화에 기여하고자 하였음.
Abstract▼
We have investigated that vitamin C has preventive effects on inhibition of hydrogen peroxide-induced gap-junctional intercellular communication (GJIC). Inhibition of GJIC and hyperphosphorylation of connexin43 induced by $H_2O_2$ was prevented by pretreatment of the cells with vitamin C.
We have investigated that vitamin C has preventive effects on inhibition of hydrogen peroxide-induced gap-junctional intercellular communication (GJIC). Inhibition of GJIC and hyperphosphorylation of connexin43 induced by $H_2O_2$ was prevented by pretreatment of the cells with vitamin C. Our results suggest that the mechanistic basis for cancer preventive action of vitamin C might be related to the effects agaist the inhibition of GJIC by free radials. We investigated antioxidant and antitumor-promoting activities of major phenolic phytochemicals of apples. Apple extracts showed the protective effects against the inhibition of GJIC by $H_2O_2$ in a dose-dependent manner. Quercetin exerted the strongest protective effects among major antioxidants in apples on $H_2O_2$-induced inhibition of GJIC, following epicatechin, procyanidin B2, and vitamin C, while chlorogenic acid and phloretin had no effects. Our results indicate that cancer chemopreventive activity of apples is associated with the combined antioxidant capacity and antitumor-promoting activities of diverse antioxidants. We found that quercetin and quercetin 3-rutinoside protected $H_2O_2$-induced inhibition of GJIC which is associated with tumor promotion. Some plum varieties (Beltsville Elite B78197 and BY 69-339) that contain more quercetins exerted much stronger protective effects against $H_2O_2$-induced inhibition of GJIC than other varieties (NY 101 and NY 9) that are low quercetin. This study indicates that quercetins contribute antitumor-promoting activity of plums through the modulation of GJIC. We analyzed phenolic phytochemicals from red pine leaves and found epigallocatechin gallate (EGCG) and epigallocatechin (EGC), and catechin gallate (CG) astheir major phenolic phytochemicals. The present study also investigated whether red pine leaf extract (RP) and its phenolic phytochemicals inhibit the invasion of SK-Hep-1 human hepatocellular carcinoma cells (SK-Hep-1 cells). RP suppressed the invasion and the migration of SK-Hep-1 cells. EGCG and CG also inhibited the invasion and migration. RP, EGCG, and CG suppressed both MMP-2 and MMP-9 activities. Curcumin suppressed the synthesis of prostaglandin E2, one of the major products of cyclooxygenase-2 (COX-2) in MCF10A human breast epithelial cells. The upregulation of MMP-9 was also significantly reduced by curcumin treatment. Likewise, curcumin attenuated invasiveness and motility of MCF10A cells stimulated with TPA. Curcumin blocked TPA-induced activation of extracellular signal-regulated protein kinase (ERK1/2) and NF-$\kappa$B-dependent promoter activity. Beta-amyloid (Abeta) is a main component of senile plaques in Alzheimer's disease (AD) that induces neuronal cell death.
※ AI-Helper는 부적절한 답변을 할 수 있습니다.