초록

동일인에서 자궁근종과 정상 자궁조직이 상존하므로, 같은 유전자의 발현을 비교 분석하는 좋은 모델이 된다는 생각과, 양쪽을 비교 분석하면 분명히 차이가 있는 것이 존재할 것으로 생각되어, 3,066개의 유전자를 배열한 DNA Chip 분석을 시행하였다. 분석된 유전자 중에서 목표 유전자를 선정하여 RT-PCR, western-blot 분석, 및 면역조직화학적 염색을 시행하여 발현의 차이가 자궁근종의 원인에 관여하는지를 규명하였다.

Abstract ▼

Our purpose is to evaluate the gene differential expression analysis in leiomyoma and corresponding normal myometrial tissue, using cDNA chip and make elucidate the pathopysiology. These findings contribute the possibility of gene or medical therapy as a novel approach for treatment of uterine leiom

Our purpose is to evaluate the gene differential expression analysis in leiomyoma and corresponding normal myometrial tissue, using cDNA chip and make elucidate the pathopysiology. These findings contribute the possibility of gene or medical therapy as a novel approach for treatment of uterine leiomyomas. To study the difference of gene expressions between leiomyoma and normal myometrial tissue by DNA Chip. The mixed probe was hybridized with two pieces of 3,066 double dot from a human dermal papilla cell cDNA library and scanned with a laser scanner. Validation of gene expression were performed by RT-PCR, Western-blot, and immunohistochemistry in 10 leiomyomas and corresponding normal myometrial tissues.Among many differentially expressed genes, genes with expression levels more than 3 times were found by comparing uterine leiomyoma corresponding with normal myometrial tissue. CYR 61 gene was shown to be markedly down-regulated in leiomyoma compared with the matched uterine myometrial control. Although alterations of several genes, such as mitotic checkpoint regulator, osteoblast specific factor II, PAI-1 mRNA-binding protein , HADHA, p311, Hexokinase I, II, DDX1, and cyclin G1 were identified in a significant high fraction of uterine leiomyoma compared to normal myometrial tissue. We validated differential expression of genes by RT-PCR and demonstrated overexpression of osteoblast specific factor II, HADHA, p311, and cyclin G1. We found that mRNA levels of cyclin G1 were elevated in human leiomyomas compared to their adjacent normal myometrial tissues. Consistent with elevated mRNA levels, high levels of cyclin G1 protein expression were detected in all leiomyoma samples using immunoblot analysis. Immunohistochemistry revealed that cyclin G1 islocated mainly in the nucleus in both normal myometrium and leiomyoma. However, higher levels of cyclin G1 were apparent in tumor regions compared to adjacent normal myometrial regions. These results suggest that cyclin G1 is frequently overexpressed in uterine leiomyoma in a p53-independent manner and that this abnormality could be attributed to the severe proliferation of human uterine leiomyomas.

목차 Contents

• Ⅰ. 연구계획 요약문...3
• 1. 국문요약문...3
• Ⅱ. 연구결과 요약문...4
• 1. 국문요약문...4
• 2. 영문요약문...5
• Ⅲ. 연구내용 및 결과...6
• 1. 서론...6
• 2. 연구방법 및 이론...7
• 3. 결과 및 고찰...9
• 4. 결론...15
• 5. 인용문헌...15