[보고서]심근경색 실험동물 모델에서 Insulin-like growth factor system의 조절

김인식

[국가R&D연구보고서] 심근경색 실험동물 모델에서 Insulin-like growth factor system의 조절
Regulation of IGF system in rats after experimental myocardial infarction 원문보기

보고서 정보
주관연구기관	전북대학교 Chonbuk National University
연구책임자	김인식
보고서유형	최종보고서
발행국가	대한민국
언어	한국어
발행년월	2010-04
과제시작연도	2009
주관부처	교육과학기술부
연구관리전문기관	한국연구재단 National Research Foundation of Korea
등록번호	TRKO201000014462
과제고유번호	1345105191
사업명	일반연구자지원
DB 구축일자	2013-04-18
키워드	흰쥐.관상동맥.결찰.심근경색.동물모델.진단.치료.기초자료.rat.left coronary artery.ligation.myocardial infarction.animal model.IGF system.diagnosis.therapy.basic data.

초록 ▼

흰쥐를 대상으로 심근경색 실험동물 모델을 확립하고, 심근경색이 진행되는 기간 동안 IGF system의 변동을 규명하여 갈수록 심각해지고 있는 심근경색질환의 차세대진단, 예방 및 치료법 개발에 대한 기초 자료를 제공하고자 본 연구를 수행하였다. 이러한 목적을 달성하기 위하여 다음과 같은 연구내용에 주안점을 두고 연구를 진행하였다. 흰쥐에서 왼쪽관상동맥을 결찰하여 심근경색 실험동물 모델 확립하고 심근경색시 혈액내 IGF system 변동 규명, 심근조직내 IGF system 변동 규명 및 심근조직내 IGF-I mRNA 변동을 규명하였

Abstract ▼

The aims of this research were to establish animal model for myocardial infarction,
determine the time course changes of the insulin-like growth factor system(IGFs and IGFBPs) of experimental myocardial infarction in rats, and provide a basic data of diagnosis and prevention-therapy for MI. The contents of this study are as follows; 1) Establishment of myocardial infarction model induced by coronary artery ligation in rats, 2) Elucidation of the alteration of IGF-I, IGF-II, and IGF-binding proteins in serum, 3) Time course changes on the IGF-I and IGF-II in cardiac tissue, 3) The effect of myocardial infarction on the protein expression of IGFBPs in cardiac tissue, 4) The effect of myocardial infarction on the mRNA expression of IGF-I in cardiac tissue.

목차 Contents

일반연구자지원사업 최종보고서(초안) ...1
목차 ...2
I. 연구계획 요약문 ...3
1. 국문요약문 ...3
II. 연구결과 요약문 ...4
1. 한글요약문 ...4
2. SUMMARY ...5
III. 연구내용 및 결과 ...6
1. 연구개발과제의 개요 ...6
2. 국내외 기술개발 현황 ...10
3. 연구수행 내용 및 결과 ...13
4. 목표 달성도 및 관련 분야에의 기여도 ...39
5. 연구결과의 활용계획 ...40
6. 연구과정에서 수집한 해외과학기술정보 ...41
7. 주관연구책임자 대표적 연구 실적 ...43
8. 참고 문헌 ...44
9. 연구 성과 ...47
10. 기타사항 ...48

표/그림 (41)

표 Induction of myocardial infarction in the Sprague-Dawley rat.
표 Diagrams of rat left coronary artery
표 Casting and diagram of left coronary artery in a SD rat.
표 The types of branching patterns of left coronary artery in the male hearts of the 2 species of rats
표 The numbers of the left ventricular branches of LCA and the their mean distances from LCA origin on its types in the hearts of male SD rats.
표 The types (type I: A, type Ⅱ: B, and type Ⅲ: C) of branching patterns (left) and corresponding graphs of frequency distribution of LV branches of left coronary artery (right) in SD male rats.
표 Sample of the infarction of heart at 4 weeks after surgery and the each numbers of ventricular branches of left coronary artery in a SD rat.
표 Changes of body weight (g) at 1, 3, 5, 7, 14 and 30 days after sham ligation (S) and ligation of left coronary artery (M).
표 Myocardial infarction size (%) at 1, 3, 5, 7, 14 and 30 days after sham ligation (S) and ligation of left coronary artery (M).
표 Changes of Heart weight (g) at 1, 3, 5, 7, 14 and 30 days after sham ligation (S) and ligation of left coronary artery (M).
표 Changes of Heart weight (mg)/Body weight (g) at 1, 3, 5, 7, 14 and 30 days after sham ligation (S) and ligation of left coronary artery (M).
표 Changes of Mean Arterial Pressure (mmHg) at 1, 3, 5, 7, 14 and 30 days after sham ligation (S) and ligation of left coronary artery (M).
표 Changes of Systolic Arterial Pressure (mmHg) at 1, 3, 5, 7, 14 and 30 days after sham ligation (S) and ligation of left coronary artery (M).
표 Changes of Diastolic Arterial Pressure (mmHg) at 1, 3, 5, 7, 14 and 30 days after sham ligation (S) and ligation of left coronary artery (M).
표 Changes of Heart Rate (beat/min) at 1, 3, 5, 7, 14 and 30 days after sham ligation (S) and ligation of left coronary artery (M).
표 Representative photos at 1 day after ligation of left coronary artery. Heart sections were stained by TTC. White; the nfarction area, Red; the intact area
표 Light micrographs of paraffin embedded sham (a), and MI hearts from 1 days (b), 3 days (c), 7 days (d), 14 days (e) and 30 days (f) after MI operation rats. (H-E stain)
표 Light micrographs of paraffin embedded sham (a). and MI hearts from 1 days (b), 3 days (c), 7 days (d), 14 days (e), and 30 days (f) after MI operation rats. (H-E stain × 25)
표 Light micrographs of paraffin embedded sham (a), and MI hearts from 1 days (b), 3 days (c), 7 days (d), 14 days (e), and 30 days (f) after MI operation rats. (Masson’s Trichrome stain × 25)
표 Light micrographs of paraffin embedded sham (a), and MI hearts from 1 days (b), 5 days (c), 7 days (d), 14 days (e) and 30 days (f) after MI operation rats. (H-E stain × 50)
표 Light micrographs of paraffin embedded sham (a), and MI hearts from 1 days(b), 5 days(c), 7 days(d), 14 days (e), and 30 days (f) after MI operation rats. (Masson’s Trichrome stain × 50)
표 Changes of interstitial fibrosis (collagen volume %) in noninfarcted myocardium of left ventricle at 1, 3, 5, 7, 14, and 30 days after sham ligation and ligation of left coronary artery.
표 Echocardiographic data at 21 day after sham and MI rats.
표 The effects of myocardial infarction on echocardiographic measurements
표 Expression of HIF-α, Caspase-8, Bax and Cytochrome-C by western blot analysis.
표 Distribution of Neutrophil (short arrows), Macrophage (arrow heads), Lymphocyte (long arrows), Myofibroblast and collagen at the site of 1 day (a), 3 days (b), 5 days (c), 7 days (d) 14 days (e) and 30 days (f) after MI. (H-E stain × 400).
표 Infiltration of neutrophils (mm2) at 1, 3, 5, 7, 14 and 30 days after ligation of left coronary artery (M).
표 Infiltration of marcrophages (mm2) at 1, 3, 5, 7, 14 and 30 days after ligation of left coronary artery (M).
표 Infiltration of lymphocyte (mm2)at 1, 3, 5, 7, 14 and 30 days after ligation of left coronary artery (M).
표 Infiltration of cells (neutrophils, macrophage and lymphocytes each cell's sum, mm2) at 1, 3, 5, 7, 14 and 30 days after ligation of left coronary artery (M).
표 대조군(Sham)과 실험군(MI)에서의 creatine kinase (CK, IU/ℓ)의 평균 수치(**, p < 0.01).
표 대조군(Sham)과 실험군(MI)에서의 creatine kinase isoenzyme MB (IU/ℓ)의 평균 수치 (**, p < 0.01).
표 대조군(Sham)과 실험군(MI)에서의 LDH(IU/ℓ)의 평균 수치(*, p < 0.05; **, p < 0.01).
표 Serum concentrations of IGF-I (ng/ml) in myocardial infarcts at different time points.
표 Serum concentrations of IGF-II (ng/ml) in myocardial infarcts at different time points. Values are mean ± S.E.M. * < 0.05 as compared to sham operated rats.
표 Serum concentrations of IGFBP-1 (ng/ml) in myocardial infarcts at different time points. Values are mean ± S.E.M. * < 0.05 as compared to sham operated rats.
표 Serum concentrations of IGFBP-3 (ng/ml) in myocardial infarcts at different time points. Values are mean ± S.E.M. * < 0.05 as compared to sham operated rats.
표 IGF-I concentrations (ng/g) of cardiac tissue in myocardial infarcts at different time points. Values are mean ± S.E.M. * < 0.05 as compared to sham operated rats.
표 IGF-II concentrations (ng/g) of cardiac tissue in myocardial infarcts at different time points. Values are mean ± S.E.M. * < 0.05 as compared to sham operated rats.
표 IGFBP-1 and IGFBP-3 expressions in cardiac tissue after myocardial infarction.
표 Left ventricle of the heart, Detection of IGF-I mRNA S, sham group; I, infarction of LV in MI group; I, non-infarction of LV in MI group

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연합인증

[국가R&D연구보고서] 심근경색 실험동물 모델에서 Insulin-like growth factor system의 조절
Regulation of IGF system in rats after experimental myocardial infarction 원문보기