초록 ▼

제 1 절 동자꽃 品種育成 硏究
1. 동자꽃 自生및 變異種수집 및 선발
동자꽃 자생종은 96년 7월부터 강원도 각지역 및 自生花재배농가에서 수집하여 주황색, 적색, 백색등 5계통을 분류하여 계통으로 유지 하였으며 주요형질을 검정하여 선발 분류에 사용하였다.
2. Lychnis속 特性檢定, 交雜親和力및 F1 種子生産
동자꽃 自生變異種5系統과 제비동자꽃, 외국종 L. viscaris등 14화종의 종자를 도입하여 파종후 1차이식하여 育苗하였다. 각 화종별로 特性檢定을 실시하고 교배는 꽃봉우리 상태에서 除雄하여 주두에

제 1 절 동자꽃 品種育成 硏究
1. 동자꽃 自生및 變異種수집 및 선발
동자꽃 자생종은 96년 7월부터 강원도 각지역 및 自生花재배농가에서 수집하여 주황색, 적색, 백색등 5계통을 분류하여 계통으로 유지 하였으며 주요형질을 검정하여 선발 분류에 사용하였다.
2. Lychnis속 特性檢定, 交雜親和力및 F1 種子生産
동자꽃 自生變異種5系統과 제비동자꽃, 외국종 L. viscaris등 14화종의 종자를 도입하여 파종후 1차이식하여 育苗하였다. 각 화종별로 特性檢定을 실시하고 교배는 꽃봉우리 상태에서 除雄하여 주두에 꽃가루를 묻혀 교배하였는데 각 系統별 종간 교배를 交互로 하여 交雜親和力을 검정하고 F1종자를 생산하였다.
3. 동자꽃 F1 特性檢定및 系統선발
2년차에서 선발된 동자꽃과 제비동자꽃의 자생변이종, $F_1$ 및 외국 도입종 등 14 화종을 Pot 정식하여 특성을 검정하고 각 系統別로 selfing, seebing 및 交配하여 각 형질별로 재차 선발을 실시하였고 각화종 및 系統別생육특성을 조사하고 종자를 採種하였다.
4. 盆花用有望系統選拔
선발된 동자꽃 40系統, F1 10계통, F2 28계통 등 78계통을 화종 파종하여 각 계통별 생육상황을 조사하고 유망系統을 선발하였다. 계통별 생육특성 조사는 UPOV 基準을 참고로 작성된 작물신품종의 출원 및 심사를 위한 특성조사기준(화훼편) 을 참고로 하여 조사하였고 유망系統의 선발은 다양한 화색, 내도복성, 다측지성, 개화기, 화경의 크기등 盆花用에 맞는 요건을 갖춘 형질을 기준으로 선발하였다.
제 2 절 自生花卉盆花생 산기술 개발
1. DIF를 이용한 自生花卉생육 및 개화조절
DIF (Difference between day and night temperature) 및 ADT (Average daily temperature)를 이용한 自生花卉類의 생육이나 형태형성에 미치는 영향을 알아보고자 솔나리는 16, 20, $24^{\circ}C$로, 제비동자꽃은 16, 20, 24, $28^{\circ}C$로 주야온도를 조합처리하였다.
2. 自生花卉盆花생산용 床土개발
自生花의 盆花생산을 위한 플러그용 育苗상토와 미니盆花에 적합한 상토를 구명하여 商品化율을 높이고자 동자꽃, 제비동자꽃, 솔나리를 공시하여 상토종류및 혼합비율을 달리하여 시험을 수행하였다.
3. 自生花卉盆花商品 향상연구
제비동자꽃과 동자꽃의 생육 및 개화조절을 위하여 적심 및 Ancymidol외 6종의 생장조절제를 토양 관주처리하여 생육상황과 꽃의 특성을 조사하였다.
제 3 절 自生花卉 조경용 地被植物 개발
1. 자생지 환경조사
地被植物로 활용가능한 몇가지 自生花의 자생지의 광환경, 토양환경 등을 조사하여 自生花별 적정재배조건을 검토하였다.
2. 차광정도가 自生地被植物의 피복도와 생육에 미치는 영향
식재시 광환경별 自生花의 내음성과 식재간격을 알아보기 위해 무차광, 35%, 55%, 75% 차광망을 설치하고 이러한 광환경에서 自生地被植物의 생육 및 피복도를 측정하였다.
3. 내공해성 검정
도심에서 발생되는 공해가스인 $O_3$과 $SO_2$가스에 대한 내성을 시험하기위해 챔버내에서 일정한 농도를 살포하고 이에 대한 내성정도를 검정한후 이를 가로화단에 심어 실증하는 시험을 실시하였다.
4. 뗏장식 育苗상토 구명시험
地被植物중 포복지를 가지는 화종을 중심으로 일반 화분재배가 아닌 뗏장화 시켜 농가재배와 造景工事시 편이 향상을 위해 뗏장 育苗床土를 구명하였다.
제 4 절 자생 화훼 稀貴種대량 번식 기술 개발
본 연구는 개발가치와 可能性을 지닌 솔나리와 금낭화 제비동자꽃의 組織培養을 통한 大量增殖方法체계를 확립하기 위하여 실시하였다. 솔나리 기내배양시 대량증식에 최적조건을 구명하기 위하여, 적정 치상조직, 배지종류, 생장조절물질종류 및 농도를 구명하였으며, 인편절편체 및 각 조직 절편체에서 캘러스, 인편형성을 조사하였다. 분화된 식물체의 토양활착율에 최적토양조건이 구명되었고, PCR 기술을 이용한 RAPD분석에 의한 유전적 변이성여부를 검정하여 변이체를 획득하였다. 금낭화의 캘러스 형성과 체세포배의 형성, multiple shoot의 분화조건이 구명되어, 1개의 치상조직으로부터 36개의 multiple shoot 분화되는 조건을 찾았다. 제비동자꽃의 각 조직편에 따른 캘러스 형성율과 multiple shoot의 형성 최
적조건이 구명되어 1개의 치상조직에서 60개 이상의 마디를 생산하여 대량증식체계를 확립하였다.

Abstract ▼

Subtitle I. The studies on breeding of Lychn is cognata Max.
This study was conducted to breed variet ies having characters of various flower color, lodging resistance, dwarf and multi- branch type for commercial pot flower using native Lychnis cog nata Max. and foreign wild type species.
1. N

Subtitle I. The studies on breeding of Lychn is cognata Max.
This study was conducted to breed variet ies having characters of various flower color, lodging resistance, dwarf and multi- branch type for commercial pot flower using native Lychnis cog nata Max. and foreign wild type species.
1. Native Ly chnis cognata Max. and foreign wild type species collected at Kangwondo and German Jellito Staudensamen GmbH company. 5 variant lines of Lychnis cognata Max. and 3 lines of separation gerneration of L.x haag eana favorable as pot flower was selected.
2. Seed germination of the major part of species were greater than 80%, but 5 species containing L. yunanens is, were lower than 40%. Cross compatability ratio in artificial pollination were poorly 10- 20%.
3. Thirteen lines and six $F_2$ having characters of various flower color, flowering, lodging resistance, dwarf and multi- branch type for commercial pot flower were selected.
4. Also five $F_1$ hybrid were selected at crossing some lines
Subtitle II. Development of techniques for pot production of native flowers.
This studies were conduct ed to invest igate the effect of difference between day and night temperature(DIF ), various substrate composit ions and mixing ratio, and the effect of pinching or growth regulators on the plant growth and flowering, in order to appreciate the possibility of utilization as a commercial pot plant in native flowers in Korea.
1. At anthesis, plant height and stem diameter had no differences between all treaments in Lilium cernum Komorov.
But plant height elongation rate and earlier flowering were promoted with increasing ADT. Regulation of flowering was possible by utilization of ADT in L ilium cernum Komorov. Plant height and flowering of Lychnis wilf ordii had no differences between all treaments, and showed no effect of ADT or DIF.
2. It was considered that substrate compositions for plug seedling were
suitable for bio media 100%, mixture of culture media and leaf mold(5:5), and mixtur of vermiculite, perlite and peatmoss (4:4:2) in Lychnis cognata and L. wilfordii. The proper substrate compositions for pot production were bio media 100% and mixture of vermiculite, perlite and peatmoss(4:4:2) for growth and flowering of L. wilfordii.
Also the best suitable substrate compositions for pot product ion were mixture of culture media and various substrates, including saw dust, leaf mold, bark, cocovita, extended chaff and fermented chaff, for plant growth and flowering of Lilium cernum Komorov.
3. Plant height were inhibited by one and two times pinching and uniconazole
0.05mg/ pot, but flowering showed no effect of pinching and growth regulators in Lychnis cognata. In ancymidol 0.25mg/ pot and PP 333 0.25mg/ pot treatement, plant height, number of branch, internode length were inhibited, and flowering were delayed 8 day s compare to no treatment in L. wilfordii.
Subtitle III. Development of ground cover plants for landscape using native flowers.
This studies were conduct ed to develop ground cover plants for landscape using native flowers. So we surveyed growth envionmental condition of native spots, change of ground cover rate and tolerance by shading degree, tolerance of urban pollut ion air($O_3$, $SO_2$) in chamber, and selecting substrates for sod product ion.
1. The most of native plants were grown in sunshine or half shading
condition. Acidities of native soil were pH 4.8~6.8. Oranic content s of native soil were very poor. we concluded that Ixeris stolonifera can live in sterile land.
2. Ground cover degrees were increased above 10 times in Dracocep halum
argunense, Elsholtzia splendens, Glechoma hederacea, Hydrocotyle sibthorpioides, Hydrocotyle ramif lora, Ixeris stolonifera, Sedum kamts chaticum, Sedum oryzifolium and Thymus magnus in two or three monthes after planting.
3. Mostly growth of native plants was good under sunshine and 35% shading.
Shading tolerance of Glechoma hederacea and Ixeris stolonifera was very excellent, so those can be planted in 0~75% shading land.

4. Photosynthetic rate were increased by $SO_2$ treatment in some native flowers. This result was concluded that $SO_2$ gases were used in sulfide metabolism.
5. Hydrocotyle sibthorpioides and Hy drocoty le ramiflora were very sensitive for urban pollution air ($O_3$, $SO_2$). So These plants can be used for indicator plants in urban.
6. Sedum oryzifolium, Hedera rhombea and Ixeris stolonifera were very tolerant for urban pollution airs ($O_3$, $SO_2$). So It was condidered that these plants can be used for planting in urban landscape.
7. The substrates mixed peat moss 60%, decomposed rice hull 30% and zeolite 10% were effective for sod production of Ixeris stolonifera, Sedum oryzifolium and Thymus magnus.
Subtitle IV. Technical development for mass propagation of rare native flowers
This studies were carried out to investigate the suitable media, salt strength of media, kinds and concentrations of plant growth regulators for establishing mass propagation system using in vitro culture of rare wild plants, Lilium cernum $K_{OMAROV}$, Dicentra spectabilis L., and Ly chnis wilfordii Max.
1. Lilium cernum $K_{OMAROV}$.
Bulb scales of Lilium cernum $K_{OM}$. were cultured on MS, $B_5$, and MS$B_5$ media containing 2,4-D, IAA, NAA, TDZ and BAP. On MS media cont aining 2,4-D 1, 2mg/$\ell$, NAA 1, 2mg/$\ell$, TDZ 0.1mg/$\ell$, and BAP 2mg/$\ell$, and on $B_5$ media containing 2,4-D 2mg/$\ell$, NAA 0.1mg/$\ell$, and TDZ 1, 2mg/$\ell$, and on MS$B_5$ media containing 2,4-D 1mg/$\ell$, NAA 0.1mg/$\ell$, TDZ 2mg/$\ell$, and BAP 2mg/$\ell$, calli were formed perfectly. Calli were formed well on MS and MS$B_5$ media supplemented with combinations of 2,4-D and TDZ or 2,4-D and BAP.
Multiple shoots and leaves from bulb scale were induced on MS, $B_5$, and MS$B_5$, media containing IAA, NAA, and BAP. Combination of 2,4-D and TDZ or 2,4- D and BAP did not induced shoots and leaves, especially when high concentration of 2,4-D were treated onto media.
Roots were induced remarkably on MS, $B_5$, and MS$B_5$ media supplemented with NAA. Growth of induced roots was also obtained on MS, $B_5$, and MS$B_5$ media containing NAA. Other growth regulators did not stimulated root induction and growth. NAA and BAP were promoted bulblet formation.
When IAA 2.0mg/$\ell$ was added into medium, the best result was obtained for bulblet formation. The bulblets placed in liquid free media resulted in good formation of shoots, roots, and bulblets. On 1/4 MS free medium, shoots, roots, and bulblets were predominantly induced. The 1/4 MS liquid medium supplemented with plant growth regulators was the best suitable condition for elongation of shoots and roots. Somatic embryos were frequently developed from embryogenic callus in liquid media with 2,4-D 1mg/$\ell$.
When leaves, roots, and bulb- scale segments were placed on MS media cont aining NAA 1mg/$\ell$ or 2,4-D 1mg/$\ell$ and various levels of sucrose concentration, the best results were obtained on MS media added 1.5% sucrose and 2,4-D 1mg/$\ell$, 3% sucrose and NAA 1mg/$\ell$, and 1.5% sucrose and NAA 1mg/$\ell$ in shoot growth and leaf differentiation. Also, in root growth and bulb formation, the better result was obtained on MS media with 6% sucrose and NAA 1mg/$\ell$.
Spermidine promoted the shoot growth and bulb formation. However, spermine promoted the leaf differentiation and root growth on MS solid media. On the MS liquid media, both spermine and spermidine stimulated organogenesis from bulb- scale segments. Agar was more effective on the regeneration of shoot, leaf, root and bulblet than gelrite was.
Regenerated plantlets were acclimatizated and grown in greenhouse in vermiculite + perlite (1:1 by volume) well. The optimal soil condition of rooting for plantlets regenerated was in peat moss.
RAPD analysis was carried out to check the genetic stability of the regenerated plants. Three polymorpic DNA fragments out of thirty - four obtained by RAPDs were observed in 12 regenerated plants using 5 decamer primers.
2. Dicentra spectabilis L. L.
Better calli were formed on MS media supplemented with combinations of 2,4-D and TDZ or 2,4-D and BAP than with single treatments.
Node was more effective explant on callus formation and shoot regeneration than leaf and stem were. Multiple shoot regeneration and better shoot growth were obtained were on MS media with BAP 2mg/$\ell$ and TDZ 0.1mg/$\ell$, respectively. Somatic embryogenesis differed depending on 2,4-D concentrations and large amount s of embryos occured in treatment of 2,4-D 1mg/$\ell$.
3. Lychnis wilfordii Max.
Leaf segments of Lychnis wilfordii Max. were cultured on MS and $B_5$ media cont aining 2,4-D, IAA, NAA, TDZ, and BAP. On MS media containing TDZ 2 mg/$\ell$, and on $B_5$ media containing NAA 2mg/$\ell$ and TDZ 2mg/$\ell$, calli were formed well. Better calli were induced on MS medium supplemented with combinations of 2,4-D and low concentration of TDZ than with single treatments. Combination of 2,4-D and high concent ration of TDZ did not induced callus. Especially, callus were formed well on B5 than MS media.
Axillary bud was not more effective on callus formation than leaf, but callus formation rate was 50% on MS media supplemented with BAP 2mg/$\ell$. Multiple shoots were induced on MS media containing BAP, and TDZ of 2mg/$\ell$. Combination of low concentration of 2,4-D and TDZ did not induced shoots, especially when IAA were treated onto media. The best result in shoot growth was obtained on MS media with TDZ 1mg/$\ell$.

목차 Contents

• 연구개발보고서 초록...1
• 提出文...2
• 要約文...3
• SUMMARY...11
• 목 차...19
• 제 1장 서 론...20
• 제 1절 연구개발의 목적과 범위...20
• 제 2절 연구의 필요성...21
• 제 2장 동자꽃 品種육성연구...22
• 제 1절 서 설...22
• 제 2절 재료 및 방법...23
• 제 3절 결과 및 고찰...28
• 제 4절 적 요...68
• 제 5절 인용문헌...69
• 제 3장 自生花卉 盆花生産 技術開發...71
• 제 1절 서 설...71
• 제 2절 재료 및 방법...73
• 제 3절 결과 및 고찰...78
• 제 4절 적 요...115
• 제 5절 인용문헌...117
• 제 4장 자생화 조경용 地被植物 개발...120
• 제 1절 서 설...120
• 제 2절 재료 및 방법...121
• 제 3절 결과 및 고찰...126
• 제 4절 적 요...166
• 제 5절 인용문헌...168
• 제 5장 自生花卉 稀貴種 대량번식기술개발...171
• 제 1절 서 설...171
• 제 2절 재료 및 방법...174
• 제 3절 결과 및 고찰...181
• 제 4절 적 요...215
• 제 5절 인용문헌...219