초록 ▼

블럭형태의 BioOss collagen을 5마리의 성견 하악 소구치 주위 제 일벽성 골결손부에 이식하여, 치주조직 재생 효과를 microCT와 조직학적 분석을 통해 관찰함.
12주간의 치유 결과, micro CT 상에서 3차원적으로 재구성했을 때, 5마리중 2마리에서 골이식재가 흩어져 결손부내에 거의 남아있지 않았음. 조직학 계측에서, 골재생량과 신생 백악질량에 있어서, 실험군이 대조군에 비해 높았으나, 통계학적 유의성은 없었음. 이는 collagen이 조기에 흡수되어. 골이식재 입자들의 결합을 돕지 못한 것으로 판단됨. 현재

블럭형태의 BioOss collagen을 5마리의 성견 하악 소구치 주위 제 일벽성 골결손부에 이식하여, 치주조직 재생 효과를 microCT와 조직학적 분석을 통해 관찰함.
12주간의 치유 결과, micro CT 상에서 3차원적으로 재구성했을 때, 5마리중 2마리에서 골이식재가 흩어져 결손부내에 거의 남아있지 않았음. 조직학 계측에서, 골재생량과 신생 백악질량에 있어서, 실험군이 대조군에 비해 높았으나, 통계학적 유의성은 없었음. 이는 collagen이 조기에 흡수되어. 골이식재 입자들의 결합을 돕지 못한 것으로 판단됨. 현재 논문 작성중임.

Abstract ▼

I. The purpose of the study was to evaluate the effect of BioOss collagen on pe riodontal regeneration of surgically created 1-wall intrabony defect in beagle dogs.
II. A total of 5 male beagle dogs, 15 months old and weighing about 10-15 kg, was chosen. The animals were divided into 2 groups: Bi

I. The purpose of the study was to evaluate the effect of BioOss collagen on pe riodontal regeneration of surgically created 1-wall intrabony defect in beagle dogs.
II. A total of 5 male beagle dogs, 15 months old and weighing about 10-15 kg, was chosen. The animals were divided into 2 groups: BioOss collagen group (right side) and sham surgery contro(left side). The mandibular third premolars were extracted prior to the experimental surgery. After 8 weeks of healing period, buccal and lingual mucoperiosteal flaps was elevated to create box-type, 4 x 5 mm, 1-wall intrabony defects at the mesial aspect of the fourth mandibular premolar teeth in right and left jaw quadrants. Then, either BioOss collagen or no material were grafted at the defects. Flaps were coronally positioned and sutured. Sutures were removed after 7 days. Dogs were sacrificed 12 weeks after surgery. Micro computed tomography of the fixed specimens were taken in order to evaluate overall dimension of the grafted site. After fixation and decalcification, the specimens will be embedded in paraffin. And it was stained with Hematoxylin and Eosin. Histologic and histomorphmetric analysis regarding the defect height, epithelial adhesion, connective tissue attachment, new cementum and bone regeneration were performed under a light microscope.
III. Overall healing was clinically uneventful. Some of BioOss particles were dissipated in the intrabony defect in the micro CT analysis. Collagen was resorbed in the early stage, and it failed to maintain the block form. In bone regeneration, a mean value of BioOss collagen?? group (1.76 ± 1.05 mm) was higher than that of control (1.16 ± 0.71 mm), but there was not significantly different. In new cementum, a mean value of BioOss collagen group (1.78 ± 1.09 mm) was also higher than that of control (1.56 ± 0.70 mm), but there was not significantly different.
IV. Prolonged retention of collagen material is required for space maintenance in further development of biomaterials for periodontal regeneration. It would be used for carrier system of growth factors. It would promote the development of domestic products, and reduce the medical cost.

목차 Contents

• 목차 ...1
• I. 총괄현황 ...2
• 1. 겉표지 ...3
• 2. 제출문 ...5
• 3. 보고서 요약서 ...6
• 4. 요약문 (한글) ...7
• 5. 요약문 (영문) ...8
• 6. 연구 성과 실적 및 향후 계획 ...9
• 7. 참여연구원 현황표 ...13
• II. 총괄연구과제 연구결과 ...14
• 1. 연구개발과제의 배경 및 필요성 ...15
• 2. 국내외 기술개발 현황 ...18
• 3. 연구개발과제의 추진체계 ...19
• 4. 연구개발수행 내용 및 결과 ...20
• 5. 목표달성도 및 관련분야 기여도 ...24
• 6. 향후 연구성과 추진 계획(※해당되는 경우만 작성) ...26
• 7. 연구개발결과의 파급효과 ...26
• 8. 연구개발결과의 활용계획 ...27
• 9. 연구개발과정에서 수집한 해외과학기술정보 ...27
• 10. 참고문헌 ...28
• III. 첨부서류 ...29
• 1. 자체평가의견서 ...30
• 2. 실적 증빙자료 ...35