초록

- 난태 휴면의 대표적 산업곤충인 누에의 휴면관련유전자 빛 단백질의 분리·정제 및 분자적 특성구명
- 누에 및 곤충의 성장·변태관련 신경호르몬 유전자의 분자생물학적 특성 및 그 기능해석
- 천·작잠의 성장관련 유전자(큐티클 단백질 유전자 및 arylphorin) 의 클로닝 및 그 기능해석
- 작잠의 휴면관련 유전자의 분리 및 기능해석

Abstract ▼

Effects of brain derived neurotrophic factor (BDNF) on the neurite growth of deutoccrcbral neurons in vitro, and production of BDNF-like neuropeptide from brain of the si lk moth, Bombyx mori were investigated. In primary cuI ture of antennal lobe (AL) neurons with BDNF, it promoted a significant ne

Effects of brain derived neurotrophic factor (BDNF) on the neurite growth of deutoccrcbral neurons in vitro, and production of BDNF-like neuropeptide from brain of the si lk moth, Bombyx mori were investigated. In primary cuI ture of antennal lobe (AL) neurons with BDNF, it promoted a significant neurite extension of putative AL projection neurons and an ourgrowth of branches from principal neurites of putative AL interneurons. Results form immunolabeling of btain and retroccrcbral complex showed that BDNF-like neuropeptide labeled allata for storage Allatotropin is a 13-residue amidated neuropeptide isolated from pharate adult heads of the tobacco hornworm, Manduca sexta and strongly slimulates biosynthesis of juvenile hormones in adults but not larval, lepidopteran corpora allata. From a Bombyx mori midgut cDNA library, a cDNA that encodes a 130-amino-acid polypeptide containing M, sexta allatotropin sequence was isolated. The B. mori allatotropin cDNA consists of 1196 nucleotides. The encoded allatotropin peptide is identical to that isolated from M. sexta and that perdicted from Pseudaletia unipuncta, wi th 84% and 81% identi ty in the amino acid sequence of the allatotropin peptide precursor, respectively.M. sexta allatotropin is flanked by two different endoprotcolytic cleavage sites within the precursor of the B. mori allatotropin peptide. Evidence from northern blotting of B. mori tissues showed that the allatotropin gene is expressed in the cells of midgut, head and integument with different transcription amount, but not in the fat bpdy and silk gland. Midgut has also anumber of allatotropin-immunoreactive cells and nerve fivers. These results will provIde valuable information in understanding the AT gens of insects.

목차 Contents

• 표지 ...1
• 제출문 ...3
• 요약문 ...4
• Summary ...7
• Contents ...13
• 목차 ...14
• 제 1 장 서 론 ...15
• 제 2 장 국내외 기술개발 현황 ...16
• 제 3 장 연구개발 수행 내용 및 결과 ...19
• 제1절 누에 휴면제어 관련 유전자 및 단백질의 분리 동정 및 기능해석 ...19
• 제2절 곤충 신경호르몬 유전자의 분자생물학적 특성과 기능구명 ...40
• 제3절 천잠의 성장관련 유전자개발 및 해석 ...111
• 제4절 작잠의 휴면관련 유전자의 기능 해석 ...149
• 제 4 장 연구개발 목표달성도 및 대외기여도 ...162
• 제 5 장 연구개발 결과의 활용 계획 ...165
• 제 6 장 참고문헌 ...166