초록

제1세부과제 : 동충하초의 유전자원의 확보와 우수 자실체 형성 균주선발
제2세부과제 : 동충하초로부터 바이오 신약 물질의 정제 및 생리활성연구
제3세부과제 : 바이오 신약 물질의 구조분석 및 인공합성연두
제1협동과제 : 정제된 바이오 신약물질의 동물실험을 통한 독성연구
제2협동과제 : 정제된 바이오신약 물질의 임상 응용 연구

Abstract ▼

Characteristics of mycelial growth of Cordyceps pruinosa was observed in 22 commonly used mycological agar media in both light and dark conditions at different culture ages. The characteristics of mycelial growth varied from strain to strain and medium to medium. Culture age also affected the myceli

Characteristics of mycelial growth of Cordyceps pruinosa was observed in 22 commonly used mycological agar media in both light and dark conditions at different culture ages. The characteristics of mycelial growth varied from strain to strain and medium to medium. Culture age also affected the mycelial growth characteristics. Mycelial growth was mostly moderate to abundant in rich media, but low in poor media. The colony texture was mostly flat when grown in the light, but dark condition induced aerial hyphae. A wide range of colony color from yellowish white to light orange was observed under the light condition depending upon the medium type and culture age, whereas no color appeared in the dark culture, irrespective of medium type and culture age. The growth rate was also higher in the dark, compared to light culture. Abundant mycelial growth with darkest colony color was observed in SDAY and SMA Y agar media. Similarly, colony color was observed darkest after 3 weeks of incubation in light. Variation in the mycelial growth characteristics of C pruinosa was found useful for characterizing the phenotypic characteristics of the pure strains under the given growth conditions.
For the physiological properties of Cordyceps pruinosa , Cordyceps pruinosa was extract with methanol. And then, methanol extract was further fractionated with Hexane, Ethyl acetate, Butanol and Water. To test the antioxidative effects of Cordyceps pruinosa , the extracts were separated with several methods. HPLC was used to purify the three colors from Cordyceps pruinosa, and 3 colors were separated such as 1)pink, 2) red and 3) yellow. And their Molecular weight was 600, 580 and 560, respectively.
Also, effects of inhibition of cancer were examined. First, we tested cytotoxicity of Cordyceps pruinosa fractions on cancer cell lines and normal cell lines. And purified anticancer molecules. Tested cancer cell lines are B16FlO(melanoma), Hela (cervixcarcinoma), SKOV3(adenocarcinoma), K562 (leukemia), HepG2 (hepatoblastoma), Du1459(prostate carcinoma), MKN-45 (stomach adenocarcinoma) and A5490ung carcinoma) cells. In those cell lines, K562, Dul45 cell lines were highly prohibited by Cordyceps pruinosa Butanol fraction. And by purification step (silica gel colUmn chromatography, Sephadex.:... LH column chromatography and HPLC) , we try to separate 3 colours. And, especially, we focused on the mechanisms of cell death exhibited by Cordyceps pruinosa butanol fraction(CPBF) against human cervical adenocarcinoma cellline, HeLa. CPBF inhibited growth and induced apoptosis in HeLa cells in a dose-dependent manners determined by MTT assay, DNA fragmentation and phosphatidylserine distribution. In Western blot analysis, apoptosis in Hela cells was associated with the activation of caspase-3, -9 and proteolytic cleavage of PARP, decrease of Bcl-2/Bax protein ratio. and release of cytochrome C from mitochondria into cytosol. Apoptosis induced by CPBF could not be prevented by various antioxidants. These results show that apoptosis of CPBF toward HeLa cells is mediated by mitochondria-dependent death-signaling pathway regardless of reactive oxygen species. Thus, it indicated that CPBF may be a potential candidate in future research and development of bioactive ingredients as anticancer agents.

목차 Contents

• 표지 ...1
• 제출문 ...3
• 요약문 ...5
• SUMMARY ...11
• CONTENTS ...15
• 목차 ...17
• 제 1 장 서 론 ...19
• 제 1 절 연구개발의 목적 ...19
• 제 2 절 연구개발의 필요성 ...19
• 제 3 절 연구개발의 범위 ...21
• 제 2 장 국내외 기술개발의 현황 ...22
• 제 1 절 동충하초의 국외 연구현황 및 동향 ...22
• 제 2 절 동충하초의 국내 연구현황 및 동향 ...23
• 제 3 절 연구결과가국내외기술개발현황에서 차지하는 위치 ...24
• 제 3 장 연구개발수행 내용 및 결과 ...25
• 제 1 절 제1세부과제 연구 내용 및 결과 ...25
• 제 2 절 제2세부과제 연구 내용 및 결과 ...57
• 제 3 절 제3세부과제 연구 내용 및 결과 ...89
• 제 4 절 제1협동과제 연구 내용 및 결과 ...117
• 제 5 절 제2협동과제 연구 내용 및 결과 ...125
• 제 4 장 연구개발목표 달성도 및 대외기여도 ...141
• 제 1 절 연구개발목표 달성도 ...141
• 제 2 절 연구개발 대외 기여도 ...144
• 제 5 장 연구개발 결과의 활용계획 ...145
• 제 1 절 추가 연구의 필요성 ...145
• 제 2 절 타 연구에의 이용 ...145
• 제 3 절 기업화 추진 방향 ...146
• 제 6 장 기타 중요 변동 사항 ...147
• 제 7 장 참고 문헌 ...148