[보고서]형태 및 분자마커를 이용한 동아시아 물부추속 식물의 계통 및 생물지리학적 연구

김창균

형태 및 분자마커를 이용한 동아시아 물부추속 식물의 계통 및 생물지리학적 연구
Phylogeny and biogeography of Isoetes from East Asia based on morphological and molecular characters 원문보기

보고서 정보
주관연구기관	아주대학교 산학협력단
연구책임자	김창균
보고서유형	최종보고서
발행국가	대한민국
언어	한국어
발행년월	2011-06
과제시작연도	2010
주관부처	교육과학기술부
사업 관리 기관	한국연구재단
등록번호	TRKO201200002835
과제고유번호	1345121393
사업명	일반연구자지원
DB 구축일자	2013-05-20
키워드	동아시아.물부추속.형태.계통.생물지리.보전.East Asia.Isoetes.morphology.phylogenetic relationship.biogeography.conservation.

초록 ▼

본 연구의 목표는 동아시아에 분포하는 물부추속 식물을 대상으로 외부형태 및 해부학적, 세포학적, 분자분류학적 형질을 분석하여 (1) 분류학적 종의 한계를 명확히 규명하고, (2) 계통학적 유연 관계를 파악하고, (3) 배수체 종의 모종을 규명하고, (4) 생물지리학적 연구를 통해 이동경로를 파악하는 것이다. 동아시아 물부추속 식물의 계통 및 지리적인 기원을 밝히기 위하여 호주, 파푸아 뉴기니아, 러시아 캄챠카, 필리핀, 북미 알래스카 등에 분포하는 물부추속 식물을 포함하였으며, 핵과 엽록체의 다양한 DNA 마커를 이용하여 분석하였다

Abstract ▼

The aims of this study are to clarify their taxonomic delimitation, to assess the phylogenetic relationship of Isoetes species from eastern Asia, and to determine the parental species of polyploid based on morphological, cytological and molecular data. Also, we unravel the migration pathway according to compare the phylogenetic relationship to geographical distribution pattern of Isoetes species among East Asia, Australia, Papua New Guinea, Philippines, Kamchatka (Russia), and Alaska (North America) regions.

목차 Contents

일반연구자지원사업 최종보고서 ... 1
목차 ... 2
I. 연구 계획 요약문 ... 3
1. 국문 요약문 ... 3
II. 연구 결과 요약문 ... 4
1. 한글요약문 ... 4
2. SUMMARY ... 5
III. 연구내용 및 결과 ... 6
1. 연구개발과제의 개요 ... 6
2. 국내외 기술개발 현황 ... 12
3. 연구수행 내용 및 결과 ... 14
4. 목표 달성도 및 관련 분야에의 기여도 ... 35
5. 연구결과의 활용계획 ... 37
6. 연구과정에서 수집한 해외과학기술정보 ... 38
7. 주관연구책임자(공동연구원) 대표적 연구 실적 ... 40
8. 참고 문헌 ... 40
9. 연구 성과 ... 43
10. 기타사항(중요 연구 변경사항 등) ... 44
[별첨1] 대표연구성과 ... 45
자체평가 의견서 ... 48

표/그림 (24)

표 Habit of Isoetes coreana Chung et Choi (25 Aug. 2007 in Kangjin, Jellanam-do, South Korea). A, adult plant; B, comb lobes; C, ligule; D, megasporangium; E, megaspore; F, microsporangium; G, microsprore.
표 Dispersal pathway of Isoetes species in East Asia. (A) Dispersal direction of Isoetes in China is represented by a spatial distribution pattern from west to east in China (from Liu et al., 2004) (B) The East Asian and Australian Isoetes species formed clade and it is originated from Australian species (from Hoot et al., 2006).
표 Neighbour-joining dendrogram on 251 individuals from 16 populations of East Asian Isoetes species based on AFLP markers using PAUP 4.02 (Swofford, 1998). Branch lengths are indicated the proportion to the amount of character change along each branch (from Kim et al., 2009b).
표 Distribution pattern of Isoetes species in East Asia, Australia, and Alaska (modified from Hoot et al., 2006)
표 Comparison of diagnostic morphological characters between two new species (I. jejuensis and I. allasanensis) and the other Isoetes species from East Asia (modified from Choi et al., 2008)
표 Representative structures and characters of heterospore in Isoetes L. (refer to Jung et al., 2009). Megaspore (a-c): a, proximal-; b, lateral-; c, perspective view. Microspore (d-g): d, proximal-; e, lateral (major axis side); f, lateral (minor axis side); g, perspective view. Characters of megaspore (1-10): 1, ornamentation of proximal hemisphere; 2, diameter; 3, attachment region of radial and equatorial ridge; 4, ornamentation around equatorial ridge at distal hemisphere; 5, ornamentation of distal hemisphere; 6, proximal-; 7, distal hemisphere; 8, height; 9, radial-; 10, equatorial ridge. Characters of microspore (11-14): 11, length; 12, ornamentation of line of attachment; 13, surface; 14, line of attachment.
표 Collection data, spore ornamentation and chromosome number of plant material used for systematic evaluation of I. asiatica.
표 Voucher information used in phylogenetic and biogeographic analyses of Isoetes from East Asia.
표 Comparison of varisous characters of seven Isoetes in East Asia. Numbers in parentheses indicate rare types.
표 SEM micrographs of megaspores and microspores of Isoetes L. in South Korea and adjacent regions (Taiwan, Japan and China). 6-9, I. coreana; 10-13, I. jejuensis; 14-17, I. hallasanensis. 18-21, I. taiwanensis; 22-25, I. sinensis; 26-29, I. japonica; 30-33, I. asiatica. 6, 10, 14, 18, 22, 26, and 30, lateral view of megaspores (Scale bars = 100㎛). 7, 11, 15, 19, 23, 27, and 31, distal view of megaspores (Scale bars = 100㎛). 8, 12, 16, 20, 24, 28, and 32, ultra-structure of megaspore surface (Scale bars = 10㎛). 9, 13, 17, 21, 25, 29, and 34, overview of microspore (Scale bars = 30㎛) (Jung et al., 2009).
표 Polymorphic bands generated by each of four pair primers in AFLP analysis
표 Neighbor-joining dendrogram of 96 individuals from five Isoetes species in Hokkaido, Kamchatka, and Alaska region based on 308 polymorphic AFLP markers generated using SplitsTree program. Individuals of Isoetes from unique clusters according to their region and group based on taxonomy.
표 The single most parsimonious tree derived from the rbcL, cpITS, and trnS-psbC spacer region, and the combined three regions of data for chloroplast DNA. Numbers above the branches are bootstrap values (≥ 60%). Branch lengths are proportional to number of substitutions (Kim et al., 2009).
표 Tree statistics of the nrITS, trnL-F, trnS-psbC, atpB-rbcL, and combined datasets from maximum parsimony (MP) analysis
표 Strict consensus tree of 184 most parsimonious tree (tree length = 318, CI = 0.882, RI = 0.968) from parsimony analysis of nrITS sequence data. Numbers above the branches indicate support values [maximum parsimony bootstrap(BP)/Bayesian posterior probability (PP)]; a dash (-) indicates that node did not receive >70% BP by MP analysis. The geographic origin of Isoetes specimens is indicated with country codes (JK, Japan, Kyushu; CH, China, Hubei Province; JA, Japan, Aomori Prefecture; JH, Japan, Hokkaido; RS, Russia, Sakhalin; RK, Russia, Kurile; RE, Russia, Kamchatka, Elizovsky; RP, Russia, Kamchatka Paratunka; RN, Russia, Kamchatka, Narichevo; UC, USA, Alaska, Cohoe lake; UJ, USA, Alaska, Jewel lake; CB, Canada, British Columbia; UN, USA, New Hampshire; RM, Russia, Magadan; UB, USA, Hawaii, Big Island; UM, USA, Hawaii, West Maui; RC, Russia, Kamchatka, Commander Island; GP, German, West Prussia; FK, Finland, Kuusamo; SS, Sweden, Smaland.
표 Strict consensus tree of 26 most parsimonious tree (tree length = 147, CI = 0.857, RI = 0.965) from parsimony analysis of combined three chloroplast DNA sequence data. Numbers above the branches indicate support values (>70% BP) by MP analysis. See the legend of Fig. 35 for the geographic origin of Isoetes specimens
표 Strict consensus tree of 30 most parsimonious tree (tree length = 488, CI = 0.817, RI = 0.944) from parsimony analysis of combined three chloroplast DNA sequence data. Numbers above the branches indicate support values (>70% BP) by MP analysis. See the legend of Fig. 35 for the geographic origin of Isoetes specimens
표 A map showing biogeographic origin of East Asian Isoetes species. With respect to phylogenetic relationship of Isoetes species based on the nrITS sequences data, we support that the Australian species probably was a center of origin for East Asian taxa including I. coreana, I. hallasanensis, I. jejuensis, I. japonica, I. pseudojaponica, I. sinensis, I. taiwanensis, I. taiwanensis, I. yunguiensis, and I. hypsophila. Symbols I-a, I-b for the subclades are provided in Fig. 36.
표 Phylogram resulting from Bayesian analysis of LEAFY sequences data of 72 LEAFY types from 10 Isoetes species in East Asia and Australia. Numbers above nodes indicate support values (maximum parsimony bootstrap/Bayesian posterior probability); - indicates that a node was not retrieved with bootstrap value greater than 50% by MP analysis (from Kim et al., 2010b)
표 Neighbour-joining(NJ) dendrogram for 15 haplotypes from 17 populations of seven Isoetes species based on sequence data for the chloroplast DNA trnS-psbC spacer region. The scale bar represents p-distance. Number above nodes indicate support value(MP bootstrap/Bayesian posterior probality); a dash indicated that a node was not retrieved with bootstrap value greater than 50% by MP analysis (Kim et al., 2010b).
표 Minimum spanning network of 15 chloroplast DNA found in seven Isoetes from East Asia. Symbols for the species are provided in the artwork (Kim et al., 2010b).
표 Hypothetical phylogeny of seven Isoetes species from East Asia involving autopolyploid and allopolyploid speciation based on the phylogenetic analyses of nuclear and chloroplast DNA. The hypothetical relationships presented in this study represent autotetraploid origin for I. hallasanensis and allohexaploid origin for I. coreana and I. japonica. (Kim et al., 2010b)
표 Spore morphology of I. laosiensis C. Kim AJOU]). A, Overview. B, Proximal view. C, Lateral view. D, Distal view. E-H, Megaspores of I. coromandelina (India. Rajasthan, Sept 29 1995, C.D.K. Cook 5327 [K]). E, Overview (S, small size; L, large size). F, Proximal view. G, Lateral view. H, Distal view. Scale bars: A and E = 500 μm; B-D and F-H = 100 μm (Kim et al., 2010).
표 Representation of phylogeny and dispersal hypothesis of Isoetes species. Black arrows indicate hypothesis for origin (Hoot et al., 2006). Red arrow indicates dispersal hypothesis in this study. Distributions of Isoetes species are shown as numericals (1. India; 2. Laos; 3. Philippines; 4. North Australia; 5. West Australia). Clade I and II of East Asian Isoetes were performed in this project.

과제명(ProjectTitle) :	-
연구책임자(Manager) :	-
과제기간(DetailSeriesProject) :	-
총연구비 (DetailSeriesProject) :	-
키워드(keyword) :	-
과제수행기간(LeadAgency) :	-
연구목표(Goal) :	-
연구내용(Abstract) :	-
기대효과(Effect) :	-

내보내기 구분	파일저장 인쇄 메일전송
구성항목	기본정보 상세정보 관리번호, 제목(한글), 저자명(한글), 발행일자, 전자원문, 초록(한글), 초록(영문) 관리번호, 제목(한글), 제목(영문), 저자명(한글), 저자명(영문), 주관연구기관(한글), 주관연구기관(영문), 발행일자, 총페이지수, 주관부처명, 과제시작일, 보고서번호, 과제종료일, 주제분류, 키워드(한글), 전자원문, 키워드(영문), 입수제어번호, 초록(한글), 초록(영문), 목차
저장형식	Text(ASCII format) Excel format
메일정보	받는사람 (필수) @ 보내는사람 (선택) @ 제목 내용 KISTI 검색결과 이메일 서비스
안내	총 건의 자료가 검색되었습니다. 다운받으실 자료의 인덱스를 입력하세요. (1-10,000) 검색결과의 순서대로 최대 10,000건 까지 다운로드가 가능합니다. 데이타가 많을 경우 속도가 느려질 수 있습니다.(최대 2~3분 소요) 다운로드 파일은 UTF-8 형태로 저장됩니다. 파일의 내용이 제대로 보이지 않을실 때는 웹브라우저 상단의 보기 -> 인코딩 -> 자동선택 여부를 확인하십시오. ~ Text(ASCII format) Excel format

연합인증

형태 및 분자마커를 이용한 동아시아 물부추속 식물의 계통 및 생물지리학적 연구
Phylogeny and biogeography of Isoetes from East Asia based on morphological and molecular characters 원문보기