[보고서]천연염색 표준화 및 기능성 부여기술 개발

신윤숙

[국가R&D연구보고서] 천연염색 표준화 및 기능성 부여기술 개발
Standardization and functionalization of natural dyeing 원문보기

보고서 정보
주관연구기관	전남대학교 Chonnam National University
연구책임자	신윤숙
보고서유형	최종보고서
발행국가	대한민국
언어	한국어
발행년월	2011-10
과제시작연도	2010
주관부처	교육과학기술부
연구관리전문기관	한국연구재단 National Research Foundation of Korea
등록번호	TRKO201200003326
과제고유번호	1345122037
DB 구축일자	2013-04-18
키워드	분말천연염료,표준화,저장안정성,인디칸,인디고,인디루빈,기능성,NT 융합,BT 융합natural dye powder,standardization,storage stability,indican,indigo,indirubin,functionality,convergence with NT,convergence with BT

초록 ▼

연구의 목적 및 내용
● 홍화, 쪽, 자초 등 주요 천연염재의 색소 분말화 및 염색 표준화
● 분말 염료의 저장안정성 및 재현성 조사
● NT 융합한 기능성 부여 천연염색 소재 개발
● 새로운 염재 발굴 및 분말염료 개발, 유효성 및 기능성 조사
● 전통 발효 과정의 표준화를 위한 발효과정 참여 미생물 조사 및 환원능력 분석
● 쪽 식물의 인디칸 함량 분석과 인디칸 분해능이 있는 효소의 발굴과 천연 인디고 제조공정 개선
● BT 융합을 통해 천연 인디고 유도체인 붉은 색의 인디루빈의 생산 공정 개발

연구결과
● 홍화 황색 및 홍색 분말염료 제조 및 저장안정성(1년 이상) 확인, 섬유별 염색 표준화 및 색상재현성 확인 (특허등록)
● 전통 쪽(니남) 분말염료 제조 및 인디고/인디루빈 함량분석
● 쪽 분말염료를 이용한 염색 표준화 및 색상재현성, 기능성, 저장안정성(2년이상) 확인
● 전통발효염색 대체공정으로서 인디고 환원공정 개발: one-step 글루코오스 환원방법
● 해조류로부터 기능성 분말염료 제조 및 유효성, 항균성 확인 (특허출원)
● 댓잎, 귤피 등 폐자원으로부터 추출한 색소의 유효성 및 기능성 확인
● 석류 과피로부터 기능성 색소추출 및 모발염색제로 유효성 확인 (특허등록)
● 천연쪽 전통발효액내 미생물 군총 분석과 인디고 환원능 확인
● 효모와 한세눌라 균주를 이용한 천연 및 합성인디고의 환원방법 개발 (특허출원)
● 천연 쪽 식물의 인디칸 함량 (평균 9.8g/kg 쪽) 분석 완료
● 천연 쪽 식물의 인디칸에 분해능을 지닌 두 효소의 선별 및 실험실 수준의 인디고 생산능 확인 (특허등록)
● 대체염료와 고부가 가치 의약품으로서 가치를 지닌 인디루빈의 재현성 있는 생산공정확립 (특허등록)
● 온도조절기능성, 아로마테라피, 피부보습성 쪽염색 소재 시작품 제작
● 효소 전처리에 의한 자초색소 분말염료 제조 및 저장안정성(6개월) 확인
● 생쪽잎 분말염료 제조 및 염색 공정 개발, 저장안정성(1년) 확인 (특허등록)

연구결과의 활용계획
● 천연 쪽을 이용한 완전한 친환경 효소 공정의 도입 가능성을 실제 적용을 통해 판단함
● 인디칸 분해능이 있는 효소의 접목을 통한 천연 발효공정의 시간 단축과 표준화 가능성을 활용 시도
● 천연 발효액에서 발굴한 균주를 이용한 인디고 환원 가능성 실제 적용 계획
● 고부가 가치 의약품으로서 잠재능력이 큰 인디루빈을 의약품과 화장품에 적용 시도
● 생쪽잎 분말염료를 초중등학교 염색실험 교구품으로 상품화
● 등록한 특허 기술 이전

Abstract ▼

Purpose&contents
● Preparation of colorants powder and standardization of dyeing from safflower, indigo plant and gromwell, storage stability and reproducibility of dye powder
● Development of functional natural dyed textile materials by applying nanotechnology(NT)
● Screening new dye resources, preparing powder dyes and studying their efficacy for natural dyes and functionality
● Development of indigo and indirubin production process by applying biotechnology(BT)

Result
● Preparation of safflower yellow and red dyes in powder and confirmation of their storage stability(more than 1 yr), standardization of dyeing and confirmation of color reproducibility (Patent registered)
● Preparation of traditional Niram dye powder and analysis of indigo and indirubin contents
● Standardization of dyeing process and confirmation of color reproducibility, functionality and storage stability (more than 2 yrs) of Niram dye powder
● Development of eco-friendly one-step glucose reduction method as an alternative traditional indigo fermentation dyeing process
● Preparation of antimicrobial dyes from marine algae and investigation of their efficacy for practical uses (Patent pending)
● Confirmation of efficacy as natural dyes and functionality of extracted colorants from wastes such as bamboo leaves, mandarine orange peels, etc
● Development of hair colorants from pomagranate rinds (Patent registered)
● Screening bacterial community in traditional fermenting vat of natural indigo and confirming their indigo reduction capability
● development of reduction method of natural and synthetic indigo using Saccharomyces cerevisiae (Patent pending)
● Screening two enzymes capable of decomposing indican and confirming indigo production in lab scale (Patent registered)
● Development of reproducible production method of indirubin from indican (Patent registered)
● Preparation method of powder dye from fresh leaves of indigo plant and confirmation of dyeability and storage stability (Patent registered)

Expected Contribution
● Applying bacteria screened from traditional indigo fermenting vat in practical indigo reduction process
● Transfer technologies in registered patents to industry
● Applying indirubin in medicinal and cosmetic areas with high added value
● Commercialize powder dye from fresh leaves: dyeing kits as teaching tools
● Expanding application fields of natural dyes and improving product quality

목차 Contents

도약연구사업(舊 국가지정연구실) 최종보고서 ... 1
목차 ... 2
연구계획 요약문 ... 3
연구결과 요약문 ... 4
한글요약문 ... 4
SUMMARY ... 5
연구내용 및 결과 ... 6
제 1 장. 연구개발과제의 개요 ... 6
제 2 장. 국내외 기술개발 현황 ... 8
제 3 장. 연구수행 내용 및 결과 ... 10
제 4 장. 목표달성도 및 관련분야에의 기여도 ... 72
제 5 장. 연구결과의 활용계획 ... 73
제 6 장. 연구과정에서 수집한 해외과학기술정보 ... 74
제 7 장. 주관연구책임자 대표적 연구실적 ... 77
제 8 장. 참고문헌 ... 78
제 9 장. 연구성과 ... 80
제 10 장. 기타사항 ... 92
[별첨1] 대표연구성과 ... 93
자체평가의견서 ... 96

표/그림 (143)

표 Storage stability of safflower yellow powder
표 Storage stability of safflower red powder.
표 K/S values and H V/C of the of cellulose fabrics dyed with safflower yellow(1.0% owb, 40min)
표 Effect of dyeing temperature on the K/S value, L*, a*, b*, C*, ho, and H V/C values (0.6% owb, 60min)
표 Effect of dyeing time
표 Effect of dyeing concentration
표 Effect of pH on the K/S value and H V/C of fabrics dyed with safflower yellow colorants (0.6% owb, 40min, 90℃, silk 30℃)
표 K/S values, L*, a*, b*, C*, ho, and H V/C values of the fabrics dyed with safflower yellow colorants for reproducibility evaluation
표 Effect of kyeing method on the K/S values and H V/C of the fabrics dyed by ultrasonic method (0.6% owb, 40min, 70℃, pH 3.5)
표 Efect of chitosan concentration on the K/S values and H V/C of the of fabrics dyed with safflower yellow(1.0% owb, 30℃, 40min)
표 K/S retention rate(40hr); Safflower Yellow Colorants
표 Effect of dyeing temperature(dye conc.: 0.6% owb, 40℃/60 min)
표 Effect of dyeing time(dye comc.: 0.6% owb, 40℃)
표 Effect of dyeing concentration
표 Effect of pH(dye conc.:0.6% owb).
표 Effect of dyeing temperature
표 Effect of dyeing time
표 Effect of dyeing concentration
표 Effect of pH
표 Effect of pH on the color of fabrics dyed with safflower red colorants (dye conc.: 0.6% owb, 40 ℃/40 min, 1:100)
표 K/S retention rate(40hr); safflower red colorants.
표 Preparation of natural indigo powder
표 Effect of extraction condition and Ca(OH)2
표 Indigo and indirubin content in natural indigo dye depending on production condition
표 Color of prepared indigo powde
표 Effect of extraction time on the colorant powder yield(extraction pH 6.0)
표 Effect of extraction time on the dye uptake(extraction pH 6.0)
표 Effect of dyeng temperature on thedye uptake(dye conc.: 4g/l, 30min)
표 Effect of dyeing time on the dye uptake(cotton-40℃ ramie-60℃ rayon-50℃)
표 Standard dyeing conditions and dyeing properties according to the color stength
표 Colorfastness of dyed fabrics according to the color strength
표 Effect of dyeing temperature on the of dye uptake of tencel.
표 Effect of dyeing time on the dye uptake of tencel.
표 Effect of sodium hydrosulfite concentration on the dye uptake depending on dye concentration of tencel
표 Effect of irradition time of the color difference repeated dyeing of tencel
표 Effect of dyeing temperature on the dye uptake of silk.
표 Effect of dyeing time on the dye uptake of silk
표 Colorfastness of the silk fabrics dyed with natural indigo
표 K/S value and color properties of wool according to the sodium hydroxide concentration (natural indigo dye 2g/L, sodium hydrosulfite 2g/L, 60℃, 30min)
표 Effect of reduction/dyeing temperature on the dye uptake of wool.
표 Effect of reduction/dyeing time on dye uptak of wool.
표 Color reproducibility of wool fabrics dyed with natural indigo dye (dye 2g/L, sodium hydrosulfite 2g/L, 60℃, 30min)
표 Various color strength obtained using the prepared natural indigo powder with nine different fabrics
표 Effect of reduction temperature and time on color strength(indigo 4 g/L, glucose 6 g/L, Ca(OH)2 4 g/L)
표 Colorfastness of dyed fabrics according to the color strength
표 Dyeing results by bacteria.
표 Powder colorants prepared at different drying conditions
표 Yield and color of leaf powder dye according to drying method
표 Dyeing mechanism of silk dyed with fresh leaves powder.
표 The color change of leaf powders according to storage at -15℃.
표 Effect of repeat dyeing times on the color coordinates of silk fabrics dye with LP-DF powder.
표 Colorfastness of the silk fabrics dyed with LP-DF power
표 Effect of storage days on K/S value and color
표 Effect of reduction/dyeing temperature on the dye uptake of silk fabrics
표 Effect of sodium hydrosulfite concentration on the dye uptake of silk fabrics sodium
표 K/S value and color properties according to the sodium hydroxide (RT leaf powder 4g/L, sodium hydrosulfite 8g/L, bath ratio 1:100)
표 Colorfastness according to the addition of sodium hydroxide
표 Color of the dyed fabrics with fresh juice and fresh leaf powder
표 Add-on and color properties of fragrant microcapsule-treated fabrics
표 SEM photographs(x1500) of microcapsule-treated fabrics after 20 times of daundering
표 Color properties and color fastness of vitamin-E microcapsule-treated fabrics
표 Performance properties and vitamin-E amount of the fragrant microcapsule-treated fabrics
표 The amount of Vitamin E retained after laundering
표 K/S value, color properties, and color fastness of the dyed fabrics
표 Photograph of PCM microcapsule treated fabric by dot printing method
표 Add-on and performance properties of dyed and PCM microcapsule-treated fabrics
표 DSC thermogram of the PCM microcapsules
표 Thermal characteristics of the PCM microcapsule-treated fabrics
표 Effect of laundering of the heat storage capacity of the PCM microcapsule-treated fabrics
표 SEM photographs(300, x1000) of octadecane microcapsule-treated fabrics after laundering
표 Color of extracted solution with and without shaking
표 Change of solution color after sugar test
표 Vasible absorption spectra of phenol-sulfuric acid method experiments according to the change on amylase
표 Vasible absorption spectra of phenol-sulfuric acid method experiments according to the change on pectinase
표 Visible absorption spectra of phenol-sulfuric acid method experiments according to the time change on hemicellulase
표 Visible absorption spectra of phenol-sulfuric acid method experiments according to the time change on H2O.
표 Determination of sugar components by phenol-sulfuric acid method in pretreatment solvent
표 Shikonin standard
표 Prepared gromwell dye
표 Visible absorption spectra of gromwell colorant powder by pretreatment enzyme
표 FT-IR spectra of gromwell colorants by different enzyme
표 A Hntibacterial rates of Gromwell colorants
표 Dyeing effect according to pretreatment(1% (o.w.b), 80℃, 60min, 1:50)
표 The effect of colorant powder storage conditions on the dye uptake and color of wool and silk fabrics
표 Dye uptake of Wool fabric dyed with different extracts(50℃, 30min)
표 Hue and chroma of fabrics dyed with different extracts(50℃, 30min)
표 Effect of dyeing temperature on the dye uptake(wool).
표 Hue and chroma of dyed wool fabric
표 Effect of dyeing conditions on the dye uptake.
표 Effect of pH on dye uptake and color (80℃, 60min, 1:50, 1% o.w.b)
표 Change in color and dye uptake by pH adjustment with lye
표 Color change by pH and dyeing temperature (LR 1:50, 1% o.w.b, 60min)
표 잿물로 후매염하여 염색한 직물의 색상 (염색조건-액비 1:50, 1% o.w.b, 80℃, 60min, 매염조건-60℃, 30min)
표 Pictures of marine algae
표 Visible absorption spectra of algae colorants by different extraction processes
표 FT-IR spectra of brown algae colorants by different extraction processes
표 FT-IR spectra of green algae colorants by different extraction processes
표 Antibacterial rates of (a)(b) brown algae and (c)(d) green algae colorants by different extraction processes
표 K/S value of cotton, silk, wool and nylon fabrics dyed with Sargassum siliquastrum extracts by different processes
표 L*, a*, b* & H V/C value of cotton, silk, wool and nylon fabrics dyed with Sargassum siliquastrum extracts by different processes (dyeing condition: bath ratio 1:50, 80℃/60min)
표 K/S value of cotton, silk, wool and nylon fabrics dyed with Dictyota coriacea extracts by different processes
표 L*, a*, b* & H V/C value of cotton, silk, wool, nylon fabrics dyed with Dictyota coriacea extracts by different processes (dyeing condition: bath ratio 1:50, 80℃/60min)
표 K/S value of cotton, silk, wool and nylon fabricsl dyed with Ulva pertusa extracts by different processes
표 L*, a*, b* & H V/C value of cotton, silk, wool and nylon fabrics dyed with Ulva pertusa extracts by different processes(dyeing condition: bath ratio 1:50, 80℃/60min)
표 K/S value of cotton, silk, wool and nylon fabrics dyed with Codium fragile extracts by different processes
표 L*, a*, b* & H V/C value of cotton, silk, wool and nylon fabrics dyed with Codium fragile extracts by different processes(dyeing condition: bath ratio 1:50, 80℃/60min)
표 UV-vis absorption spectrum of bamboo green dye extracted with different metal types
표 Wool and microfiber nylon fabrics dyed with bamboo dye treated with different metal types (metal ion conc. 0.05%, NaOH 0.5%, pH 7, 100℃,60min)
표 K/S value of wool and microfiber nylon fabrics dyed with bamboo leaf extracts depending on Cu treatment concentration
표 K/S value of dyed wool and microfiber nylon fabrics depending on NaOH concentration of extraction
표 Structure of chlorophyll and sodium copper chlorophyllin : R-CH3(a series) R-CHO(b series)
표 Visible absorption spectra of bamboo green dye and sodium copper chlorophyllin
표 FT-IR spectra of (a) bamboo green dye and (b) sodium copper cholrophyllin
표 Effect of dye concentration on the dye uptake (1:50, 100℃, 60min, pH 7)
표 Effect of dyeing temperature on the dye uptake (1:50, 3℃, o.w.b., 60min, pH 7)
표 Effect of dyeing time on the dye uptake
표 Effect of the pH of dyebath on the dye uptake
표 Bacterial reduction rates of fabrics dyed with bamboo green dye
표 Effect of extraction solvent on dye uptake, color characteristics and light fastness
표 Dyeability of wool and silk depending on extraction method and dyeing temspteicrastureFabric Ecxotnrdaicttioionn Dteymeing
표 Freeze-dried dye from orange peels
표 Spectrum of orange peels colorants
표 Dyeability of colorants prepared from fresh orange peels (LR 1:50, 3% owb, 60min)
표 Reaction pathway of indirubin from indican in Poligonum tinctorium
표 Results of HPLC (A) and NMR (B) analysis
표 Effect of pH and temperature on conversion yields of indigo and indirubin
표 Effect of cultivation medium on indirubin yield
표 Indirubin production with various strains of E.coli (A) and sample (B)
표 Color of silk fabrics dyed with indirubin from different processes
표 Extracts from indigo plant with different solvents
표 TLC analysis of extracts
표 인디칸 정량을 위한 standard curve 작성
표 HPLC chromatogram
표 Calibration curve for quantification of indican
표 Conversion process of indican if indigo plant leaves to indigo for quantification
표 Effect of amount of leaves and enzyme.
표 Effect of surfactant concentration of the yield of indigo and dye uptake
표 Effect of Ca(OH)2 concentration on the yield of indigo and dye uptake
표 Effect of surfactant and enzyme on the color properties.
표 Zymogram staining of β-glucosidase activity with MUG (1: control 2:F. johnsoniae 3:T. thermophilus HB8, 4: S. Meliloti, 5: T. caldophilus)
표 Activity of β-glucosidase to indican and the indigo plant extracts
표 Enzyme activity and stability
표 Conversion profile of indican the purified β-glucosidase.

참고문헌 (25)

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안내	총 건의 자료가 검색되었습니다. 다운받으실 자료의 인덱스를 입력하세요. (1-10,000) 검색결과의 순서대로 최대 10,000건 까지 다운로드가 가능합니다. 데이타가 많을 경우 속도가 느려질 수 있습니다.(최대 2~3분 소요) 다운로드 파일은 UTF-8 형태로 저장됩니다. 파일의 내용이 제대로 보이지 않을실 때는 웹브라우저 상단의 보기 -> 인코딩 -> 자동선택 여부를 확인하십시오. ~ Text(ASCII format) Excel format

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