[보고서]유전자재조합식품의 정량시험법 검증 연구

남용석

보고서 정보

주관연구기관

(주)코젠바이오텍
Kogene Biotech co.Ltd

연구책임자

남용석

보고서유형

최종보고서

발행국가

대한민국

언어

한국어

발행년월

2011-11

과제시작연도

2011

주관부처

식품의약품안전청

사업 관리 기관

식품의약품안전청
Korea Food & Drug Administration

등록번호

TRKO201200007112

과제고유번호

1475006372

DB 구축일자

2013-05-20

키워드

유전자재조합식품,정량시험법,실시간 중합효소연쇄반응genetically modified food,quantification assay,real time PCR

표 Certified reference materials purchased from Sigma and AOCS
표 Dilution of Calibrator DNA for TC1507
표 Dilution of Calibrator DNA for NK603
표 Dilution of Calibrator DNA for MON863
표 Dilution of Calibrator DNA for DAS-59122-7
표 Dilution of Calibrator DNA for Event 3272
표 Dilution of Calibrator DNA for DP98140
표 Dilution of Calibrator DNA for MIR604
표 Dilution of Calibrator DNA for MON88017
표 Dilution of Calibrator DNA for MON89034
표 Dilution of Calibrator DNA for MIR162
표 The Guideline of CODEX and JRC
표 Primers and probes for the quantitative analysis of TC1507
표 Real-time PCR reaction mixture for the quantitative analysis of maize reference gene, Hmg
표 Real-time PCR reaction mixture for the quantitative analysis of TC1507
표 Condition of Real-time PCR analysis for Hmg and TC1507
표 Primers and probes for the quantitative analysis of TC1507
표 Real-time PCR reaction mixture for the quantitative analysis of maize reference gene, adh1
표 Real-time PCR reaction mixture for the quantitative analysis of NK603
표 Condition of Real-time PCR analysis for adh1 and NK603
표 Primers and probes for the quantitative analysis of MON863
표 Real-time PCR reaction mixture for the quantitative analysis of maize reference gene, adh1
표 Real-time PCR reaction mixture for the quantitative analysis of MON863
표 Condition of Real-time PCR analysis for adh1 and MON863
표 Primers and probes for the quantitative analysis of DAS-59122-7
표 Real-time PCR reaction mixture for the quantitative analysis of maize reference gene, Hmg
표 Real-time PCR reaction mixture for the quantitative analysis of DAS-59122-7
표 Condition of Real-time PCR analysis for Hmg and DAS-59122-7
표 Primers and probes for the quantitative analysis of Event 3272
표 Real-time PCR reaction mixture for the quantitative analysis of maize reference gene, adh1
표 Real-time PCR reaction mixture for the quantitative analysis of Event 3272
표 Condition of Real-time PCR analysis for adh1 and Event 3272
표 Primers and probes for the quantitative analysis of DP98140
표 Real-time PCR reaction mixture for the quantitative analysis of maize reference gene, Hmg
표 Real-time PCR reaction mixture for the quantitative analysis of DP98140
표 Condition of Real-time PCR analysis for Hmg and DP98140
표 Primers and probes for the quantitative analysis of MIR604
표 Real-time PCR reaction mixture for the quantitative analysis of maize reference gene, adh1
표 Real-time PCR reaction mixture for the quantitative analysis of MIR604
표 Condition of Real-time PCR analysis for adh1 and MIR604
표 Primers and probes for the quantitative analysis of MON88017
표 Real-time PCR reaction mixture for the quantitative analysis of maize reference gene, Hmg
표 Real-time PCR reaction mixture for the quantitative analysis of MON88017
표 Condition of Real-time PCR analysis for adh1 and MON88017
표 Primers and probes for the quantitative analysis of MON89034
표 Real-time PCR reaction mixture for the quantitative analysis of maize reference gene, Hmg
표 Real-time PCR reaction mixture for the quantitative analysis of MON89034
표 Condition of Real-time PCR analysis for adh1 and MON89034
표 Primers and probes for the quantitative analysis of MIR162
표 Real-time PCR reaction mixture for the quantitative analysis of maize reference gene, adh1
표 Real-time PCR reaction mixture for the quantitative analysis of MIR162
표 Condition of Real-time PCR analysis for adh1 and MIR162
표 Ct value, SD and % RSD of the maize reference gene, Hmg assay performed by Lab. 1*
표 Ct value, SD and % RDS of the TC1507 event-specific assay performed by Lab. 1
표 Mean value of GM % of unknown GMO samples performed by Lab. 1
표 Amplification plots and standard curves for TC1507 event-specific quantitative Real-time PCR method using gradient-diluted TC1507 CRM genomic DNA as the template, performed by Lab. 1. A. Amplification graph and standard curves for the maize reference gene, Hmg assay. B. Amplification graph and standard curves for the TC1507-event specific assay.
표 Ct value, SD and % RSD of the maize reference gene, Hmg assay performed by Lab. 2*
표 Ct value, SD and % RDS of the TC1507 event-specific assay performed by Lab. 2
표 Mean value of GM % of unknown GMO samples performed by Lab. 2
표 Amplification plots and standard curves for TC1507 event-specific quantitative Real-time PCR method using gradient-diluted TC1507 CRM genomic DNA as the template, performed by Lab. 2. A. Amplification graph and standard curves for the maize reference gene, Hmg assay. B. Amplification graph and standard curves for the TC1507-event specific assay.
표 Ct value, SD and % RSD of the maize reference gene, Hmg assay performed by Lab. 3*
표 Ct value, SD and % RDS of the TC1507 event-specific assay performed by Lab. 3
표 Mean value of GM % of unknown GMO samples performed by Lab. 3
표 Amplification plots and standard curves for TC1507 event-specific quantitative Real-time PCR method using gradient-diluted TC1507 CRM genomic DNA as the template, performed by Lab. 3. A. Amplification graph and standard curves for the maize reference gene, Hmg assay. B. Amplification graph and standard curves for the TC1507-event specific assay.
표 Mean values of measured GM % determined in the collaborative trial.
표 The positive rate of TC1507 event specific amplification
표 TC1507 validation data and method performance by slop, PCR efficiency and Linearity(R²)
표 Ct value, SD and % RSD of the maize reference gene, adh1 assay performed by Lab. 1
표 Ct value, SD and % RDS of the NK603 event-specific assay performed by Lab. 1
표 Mean value of GM % of unknown GMO samples performed by Lab. 1
표 Amplification plots and standard curves for NK603 event-specific quantitative Real-time PCR method using gradient-diluted NK603 CRM genomic DNA as the template, performed by Lab. 1. A. Amplification graph and standard curves for the maize reference gene, Hmg assay. B. Amplification graph and standard curves for the NK603-event specific assay.
표 Ct value, SD and % RSD of the maize reference gene, adh1 assay performed by Lab. 2
표 Ct value, SD and % RDS of the NK603 event-specific assay performed by Lab. 2
표 Mean value of GM % of unknown GMO samples performed by Lab. 2
표 Amplification plots and standard curves for NK603 event-specific quantitative Real-time PCR method using gradient-diluted NK603 CRM genomic DNA as the template, performed by Lab. 2. A. Amplification graph and standard curves for the maize reference gene, Hmg assay. B. Amplification graph and standard curves for the NK603-event specific assay.
표 Ct value, SD and % RSD of the maize reference gene, Hmg assay performed by Lab. 3
표 Ct value, SD and % RDS of the NK603 event-specific assay performed by Lab. 3
표 Mean value of GM % of unknown GMO samples performed by Lab. 3
표 Amplification plots and standard curves for NK603 event-specific quantitative Real-time PCR method using gradient-diluted NK603 CRM genomic DNA as the template, performed by Lab. 3. A. Amplification graph and standard curves for the maize reference gene, Hmg assay. B. Amplification graph and standard curves for the NK603-event specific assay.
표 Mean values of measured GM % determined in the collaborative trial
표 The positive rate of NK603 event specific amplification
표 NK603 validation data and method performance by slop, PCR efficiency and Linearity(R²)
표 Ct value, SD and % RSD of the maize reference gene, adh1 assay performed by Lab. 1
표 Ct value, SD and % RDS of the MON863 event-specific assay performed by Lab. 1
표 Mean value of GM % of unknown GMO samples performed by Lab. 1
표 Amplification plots and standard curves for MON863 event-specific quantitative Real-time PCR method using gradient-diluted MON863 CRM genomic DNA as the template, performed by Lab. 1. A. Amplification graph and standard curves for the maize reference gene, adh1 assay. B. Amplification graph and standard curves for the MON863-event specific assay.
표 Ct value, SD and % RSD of the maize reference gene, adh1 assay performed by Lab. 2
표 Ct value, SD and % RDS of the MON863 event-specific assay performed by Lab. 2
표 Mean value of GM % of unknown GMO samples performed by Lab. 2
표 Amplification plots and standard curves for MON863 event-specific quantitative Real-time PCR method using gradient-diluted MON863 CRM genomic DNA as the template, performed by Lab. 2. A. Amplification graph and standard curves for the maize reference gene, adh1 assay. B. Amplification graph and standard curves for the MON863-event specific assay.
표 Ct value, SD and % RSD of the maize reference gene, adh1 assay performed by Lab. 3
표 Ct value, SD and % RDS of the MON863 event-specific assay performed by Lab. 3
표 Mean value of GM % of unknown GMO samples performed by Lab. 3
표 Amplification plots and standard curves for MON863 event-specific quantitative Real-time PCR method using gradient-diluted MON863 CRM genomic DNA as the template, performed by Lab. 3. A. Amplification graph and standard curves for the maize reference gene, adh1 assay. B. Amplification graph and standard curves for the MON863-event specific assay.
표 Mean values of measured GM % determined in the collaborative trial.
표 The positive rate of MON863 event specific amplification
표 MON863 validation data and method performance by slop, PCR efficiency and Linearity(R²)
표 Ct value, SD and % RSD of the maize reference gene, Hmg assay performed by Lab. 1
표 Ct value, SD and % RDS of the DAS-59122-7 event-specific assay performed by Lab. 1
표 Mean value of GM % of unknown GMO samples performed by Lab. 1
표 Amplification plots and standard curves for DAS-59122-7 event-specific quantitative Real-time PCR method using gradient-diluted DAS-59122-7 CRM genomic DNA as the template, performed by Lab. 1. A. Amplification graph and standard curves for the maize reference gene, Hmg assay. B. Amplification graph and standard curves for the DAS-59122-7-event specific assay.
표 Ct value, SD and % RSD of the maize reference gene, Hmg assay performed by Lab. 2
표 Ct value, SD and % RDS of the DAS-59122-7 event-specific assay performed by Lab. 2
표 Mean value of GM % of unknown GMO samples performed by Lab. 2
표 Amplification plots and standard curves for DAS-59122-7 event-specific quantitative Real-time PCR method using gradient-diluted DAS-59122-7 CRM genomic DNA as the template, performed by Lab. 2. A. Amplification graph and standard curves for the maize reference gene, Hmg assay. B. Amplification graph and standard curves for the DAS-59122-7-event specific assay.
표 Ct value, SD and % RSD of the maize reference gene, Hmg assay performed by Lab. 3
표 Ct value, SD and % RDS of the DAS-59122-7 event-specific assay performed by Lab. 3
표 Mean value of GM % of unknown GMO samples performed by Lab. 3
표 Amplification plots and standard curves for DAS-59122-7 event-specific quantitative Real-time PCR method using gradient-diluted DAS-59122-7 CRM genomic DNA as the template, performed by Lab. 3. A. Amplification graph and standard curves for the maize reference gene, Hmg assay. B. Amplification graph and standard curves for the DAS-59122-7-event specific assay.
표 Mean values of measured GM % determined in the collaborative trial.
표 The positive rate of DAS-59122-7 event specific amplification
표 DAS-59122-7 validation data and method performance by slop, PCR efficiency and Linearity(R²)
표 Ct value, SD and % RSD of the maize reference gene, adh1 assay performed by Lab. 1
표 Ct value, SD and % RDS of the Event3272 event-specific assay performed by Lab. 1
표 Mean value of GM % of unknown GMO samples performed by Lab. 1
표 Amplification plots and standard curves for Event3272 event-specific quantitative Real-time PCR method using gradient-diluted Event3272 CRM genomic DNA as the template, performed by Lab. 1. A. Amplification graph and standard curves for the maize reference gene, adh1 assay. B. Amplification graph and standard curves for the Event3272-event specific assay.
표 Ct value, SD and % RSD of the maize reference gene, adh1 assay performed by Lab. 2
표 Ct value, SD and % RDS of the Event3272 event-specific assay performed by Lab. 2
표 Mean value of GM % of unknown GMO samples performed by Lab. 2
표 Amplification plots and standard curves for Event3272 event-specific quantitative Real-time PCR method using gradient-diluted Event3272 CRM genomic DNA as the template, performed by Lab. 2. A. Amplification graph and standard curves for the maize reference gene, adh1 assay. B. Amplification graph and standard curves for the Event3272-event specific assay.
표 Ct value, SD and % RSD of the maize reference gene, adh1 assay performed by Lab. 3
표 Ct value, SD and % RDS of the Event3272 event-specific assay performed by Lab. 3
표 Mean value of GM % of unknown GMO samples performed by Lab. 3
표 Amplification plots and standard curves for Event3272 event-specific quantitative Real-time PCR method using gradient-diluted Event3272 CRM genomic DNA as the template, performed by Lab. 3. A. Amplification graph and standard curves for the maize reference gene, adh1 assay. B. Amplification graph and standard curves for the Event3272-event specific assay.
표 Mean values of measured GM % determined in the collaborative trial.
표 The positive rate of Event3272 event specific amplification
표 Event3272 validation data and method performance by slop, PCR efficiency and Linearity(R²)
표 Ct value, SD and % RSD of the maize reference gene, adh1 assay performed by Lab. 1
표 Ct value, SD and % RDS of the DP98140 event-specific assay performed by Lab. 1
표 Mean value of GM % of unknown GMO samples performed by Lab. 1
표 Amplification plots and standard curves for DP98140 event-specific quantitative Real-time PCR method using gradient-diluted DP98140 CRM genomic DNA as the template, performed by Lab. 1. A. Amplification graph and standard curves for the maize reference gene, adh1 assay. B. Amplification graph and standard curves for the DP98140-event specific assay.
표 Ct value, SD and % RSD of the maize reference gene, adh1 assay performed by Lab. 2
표 Ct value, SD and % RDS of the DP98140 event-specific assay performed by Lab. 2
표 Mean value of GM % of unknown GMO samples performed by Lab. 2
표 Amplification plots and standard curves for DP98140 event-specific quantitative Real-time PCR method using gradient-diluted DP98140 CRM genomic DNA as the template, performed by Lab. 2. A. Amplification graph and standard curves for the maize reference gene, adh1 assay. B. Amplification graph and standard curves for the DP98140-event specific assay.
표 Ct value, SD and % RSD of the maize reference gene, adh1 assay performed by Lab. 3
표 Ct value, SD and % RDS of the DP98140 event-specific assay performed by Lab. 3
표 Mean value of GM % of unknown GMO samples performed by Lab. 3
표 Amplification plots and standard curves for DP98140 event-specific quantitative Real-time PCR method using gradient-diluted DP98140 CRM genomic DNA as the template, performed by Lab. 3. A. Amplification graph and standard curves for the maize reference gene, adh1 assay. B. Amplification graph and standard curves for the DP98140-event specific assay.
표 Mean values of measured GM % determined in the collaborative trial.
표 The positive rate of DP98140 event specific amplification
표 DP98140 validation data and method performance by slop, PCR efficiency and Linearity(R²)
표 Ct value, SD and % RSD of the maize reference gene, adh1 assay performed by Lab. 1
표 Ct value, SD and % RDS of the MIR604 event-specific assay performed by Lab. 1
표 Mean value of GM % of unknown GMO samples performed by Lab. 1
표 Amplification plots and standard curves for MIR604 event-specific quantitative Real-time PCR method using gradient-diluted MIR604 CRM genomic DNA as the template, performed by Lab. 1. A. Amplification graph and standard curves for the maize reference gene, adh1 assay. B. Amplification graph and standard curves for the MIR604-event specific assay.
표 Ct value, SD and % RSD of the maize reference gene, adh1 assay performed by Lab. 2
표 Ct value, SD and % RDS of the MIR604 event-specific assay performed by Lab. 2
표 Mean value of GM % of unknown GMO samples performed by Lab. 2
표 Amplification plots and standard curves for MIR604 event-specific quantitative Real-time PCR method using gradient-diluted MIR604 CRM genomic DNA as the template, performed by Lab. 2. A. Amplification graph and standard curves for the maize reference gene, adh1 assay. B. Amplification graph and standard curves for the MIR604-event specific assay.
표 Ct value, SD and % RSD of the maize reference gene, adh1 assay performed by Lab. 3
표 Ct value, SD and % RDS of the MIR604 event-specific assay performed by Lab. 3
표 Mean value of GM % of unknown GMO samples performed by Lab. 3
표 Amplification plots and standard curves for MIR604 event-specific quantitative Real-time PCR method using gradient-diluted MIR604 CRM genomic DNA as the template, performed by Lab. 3. A. Amplification graph and standard curves for the maize reference gene, adh1 assay. B. Amplification graph and standard curves for the MIR604-event specific assay.
표 Mean values of measured GM % determined in the collaborative trial.
표 The positive rate of MIR604 event specific amplification
표 MIR604 validation data and method performance by slop, PCR efficiency and Linearity(R²)
표 Ct value, SD and % RSD of the maize reference gene, Hmg assay performed by Lab. 1
표 Ct value, SD and % RDS of the MON88017 event-specific assay performed by Lab. 1
표 Mean value of GM % of unknown GMO samples performed by Lab. 1
표 Amplification plots and standard curves for MON88017 event-specific quantitative Real-time PCR method using gradient-diluted MON88017 CRM genomic DNA as the template, performed by Lab. 1. A. Amplification graph and standard curves for the maize reference gene, Hmg assay. B. Amplification graph and standard curves for the MON88017-event specific assay.
표 Ct value, SD and % RSD of the maize reference gene, Hmg assay performed by Lab. 2
표 Ct value, SD and % RDS of the MON88017 event-specific assay performed by Lab. 2
표 Mean value of GM % of unknown GMO samples performed by Lab. 2
표 Amplification plots and standard curves for MON88017 event-specific quantitative Real-time PCR method using gradient-diluted MON88017 CRM genomic DNA as the template, performed by Lab. 2. A. Amplification graph and standard curves for the maize reference gene, Hmg assay. B. Amplification graph and standard curves for the MON88017-event specific assay.
표 Ct value, SD and % RSD of the maize reference gene, Hmg assay performed by Lab. 3
표 Ct value, SD and % RDS of the MON88017 event-specific assay performed by Lab. 3
표 Mean value of GM % of unknown GMO samples performed by Lab. 3
표 Amplification plots and standard curves for MON88017 event-specific quantitative Real-time PCR method using gradient-diluted MON88017 CRM genomic DNA as the template, performed by Lab. 3. A. Amplification graph and standard curves for the maize reference gene, Hmg assay. B. Amplification graph and standard curves for the MON88017-event specific assay.
표 Mean values of measured GM % determined in the collaborative trial.
표 The positive rate of MON88017 event specific amplification
표 MON88017 validation data and method performance by slop, PCR efficiency and Linearity(R²)
표 Ct value, SD and % RSD of the maize reference gene, Hmg assay performed by Lab. 1
표 Ct value, SD and % RDS of the MON89034 event-specific assay performed by Lab. 1
표 Mean value of GM % of unknown GMO samples performed by Lab. 1
표 Amplification plots and standard curves for MON89034 event-specific quantitative Real-time PCR method using gradient-diluted MON89034 CRM genomic DNA as the template, performed by Lab. 1. A. Amplification graph and standard curves for the maize reference gene, Hmg assay. B. Amplification graph and standard curves for the MON89034-event specific assay.
표 Ct value, SD and % RSD of the maize reference gene, Hmg assay performed by Lab. 2
표 Ct value, SD and % RDS of the MON89034 event-specific assay performed by Lab. 2
표 Mean value of GM % of unknown GMO samples performed by Lab. 2
표 Amplification plots and standard curves for MON89034 event-specific quantitative Real-time PCR method using gradient-diluted MON89034 CRM genomic DNA as the template, performed by Lab. 2. A. Amplification graph and standard curves for the maize reference gene, Hmg assay. B. Amplification graph and standard curves for the MON89034-event specific assay.
표 Ct value, SD and % RSD of the maize reference gene, Hmg assay performed by Lab. 3
표 Ct value, SD and % RDS of the MON89034 event-specific assay performed by Lab. 3
표 Mean value of GM % of unknown GMO samples performed by Lab. 3
표 Amplification plots and standard curves for MON89034 event-specific quantitative Real-time PCR method using gradient-diluted MON89034 CRM genomic DNA as the template, performed by Lab. 3. A. Amplification graph and standard curves for the maize reference gene, Hmg assay. B. Amplification graph and standard curves for the MON89034-event specific assay.
표 Mean values of measured GM % determined in the collaborative trial.
표 The positive rate of MON89034 event specific amplification
표 MON89034 validation data and method performance by slop, PCR efficiency and Linearity(R²)
표 Ct value, SD and % RSD of the maize reference gene, adh1 assay performed by Lab. 1
표 Ct value, SD and % RDS of the MIR162 event-specific assay performed by Lab. 1
표 Mean value of GM % of unknown GMO samples performed by Lab. 1
표 Amplification plots and standard curves for MIR162 event-specific quantitative Real-time PCR method using gradient-diluted MIR162 CRM genomic DNA as the template, performed by Lab. 1. A. Amplification graph and standard curves for the maize reference gene, adh1 assay. B. Amplification graph and standard curves for the MIR162-event specific assay.
표 Ct value, SD and % RSD of the maize reference gene, adh1 assay performed by Lab. 2
표 Ct value, SD and % RDS of the MIR162 event-specific assay performed by Lab. 2
표 Mean value of GM % of unknown GMO samples performed by Lab. 2
표 Amplification plots and standard curves for MIR162 event-specific quantitative Real-time PCR method using gradient-diluted MIR162 CRM genomic DNA as the template, performed by Lab. 2. A. Amplification graph and standard curves for the maize reference gene, adh1 assay. B. Amplification graph and standard curves for the MIR162-event specific assay.
표 Ct value, SD and % RSD of the maize reference gene, adh1 assay performed by Lab. 3
표 Ct value, SD and % RDS of the MIR162 event-specific assay performed by Lab. 3
표 Mean value of GM % of unknown GMO samples performed by Lab. 3
표 Amplification plots and standard curves for MIR162 event-specific quantitative Real-time PCR method using gradient-diluted MIR162 CRM genomic DNA as the template, performed by Lab. 3. A. Amplification graph and standard curves for the maize reference gene, adh1 assay. B. Amplification graph and standard curves for the MIR162-event specific assay.
표 Mean values of measured GM % determined in the collaborative trial.
표 The positive rate of MIR162 event specific amplification
표 MIR162 validation data and method performance by slop, PCR efficiency and Linearity(R²)
표 Combined trait products of GM maize
표 Producing countries of GMO and stacked events. Source: Clive James, 2009.
표 Theoretical combinations to produce new GM maize by stacking
표 Example of the qualitative assay for the stacked GM-maize by multiplex Real-time PCR method.

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유전자재조합식품의 정량시험법 검증 연구
Study on the verification of quantitative assay for genetically modified foods 원문보기

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유전자재조합식품의 정량시험법 검증 연구 Study on the verification of quantitative assay for genetically modified foods 원문보기

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유전자재조합식품의 정량시험법 검증 연구
Study on the verification of quantitative assay for genetically modified foods 원문보기

표/그림 (206)

표/그림 (206)