초록

◦ 외국의 부유진균의 관리 동향 검토
– 부유미생물의 위해성
– 외국의 부유진균 관리 기준
◦ 부유진균의 시료채취 및 분리ㆍ동정 방법
– 시료채취 시료채취 방법, 배양온도, 입도분석
– PCR에 의한 분자생물학적 분석
◦ 부유진균의 분석방법 정립
◦ 다중이용시설의 부유미생물오염도 조사
– 보육시설, 노인요양원, 지하역사 등의 총부유세균 조사
– 보육시설, 노인요양원, 지하역사 등의 부유진균조사

Abstract ▼

Recently, There is increasing interest in biopollutants of indoor environments. Fungi and their spore are one of the biopollutants that is the cause of hypersensitivity reaction, bronchial asthma, allergic rhinitis. They may be associated with reaction in populations of susceptible individuals.
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Recently, There is increasing interest in biopollutants of indoor environments. Fungi and their spore are one of the biopollutants that is the cause of hypersensitivity reaction, bronchial asthma, allergic rhinitis. They may be associated with reaction in populations of susceptible individuals.
This study was performed to evaluate the characteristics of airborne bioaerosol(bacteria & fungi) in 5 public facilities such as Kindergar-ten building, elementary school, nursing homes, Seoul subway Dongdaemun station with HVAC(heating, ventilating, and air-conditioning) system, Incheoun subway Kaesan station with HVAC system. Bacteria(TSA medium, incubated at 35) and fungi(MEA medium, incubated at 25) sample were collected with Anderson single stage sampler(28.3L/min) and six stage viable particulate cascade impactor from April to December 2008. Air sampling time was for 5 to 10 minutes
As result of the survey, the mean concentration of total bacteria count of outdoor air were 305CFU/(53~938CFU/), the mean concentration of total bacteria count of indoor air were 522CFU/(101~1,563CFU/) with 5 public facilities. The mean concentration of total fungi count of outdoor air were 359CFU/(33~1,227CFU/), the mean concentration of total fungi count of indoor air were 301CFU/(25~863CFU/) with 5 public facilities and remain below WHO recommendations(500CFU/).
The mean I/O ratio of Kindergarten building, nursing homes elementary school, Seoul subway Dongdaemun station, Incheoun subway Kaesan station were 2.9, 1.0, 1.3, 1.6 and 2.2 for bacteria, 0.6, 0.7, 1.0, 1.1, and 0.8 for fungi respectively. Total bacteria count in Kindergarten were greater than the outdoor concentrations by the re-suspension of settled dust that containing bacteria. with the activities of children.

목차 Contents

• 표지 ... 1
• 요약문 ... 2
• Abstract ... 6
• 차례 ... 8
• I. 서 론 ... 14
• II. 재료 및 방법 ... 16
• 1. 조사대상시설 ... 16
• 2. 시료채취방법 ... 20
• 3. 분석방법 ... 20
• 가. 총부유세균수(TBC, total bacteria count) ... 20
• 나. 부유진균(TFC, Total fungi count) ... 21
• 4. 입경별 총부유세균 및 분유진균 ... 25
• III. 연구 결과 및 고찰 ... 26
• 1. 부유미생물(부유진균 등) 관리 동향 ... 26
• 가. 부유진균의 위해성 ... 26
• 나. 외국의 부유진균 관리기준 ... 29
• 2. 시료채취 및 분리ㆍ동정 방법 ... 31
• 가. 시료채취 유량 ... 31
• 나. 배양 온도 ... 34
• 다. 바이오에어로졸의 입경분포 ... 39
• 라. PCR 분석에 의한 진균 동정 ... 47
• 3. 다중이용시설의 부유미생물 분포 특성 ... 48
• 가. 시설별 총부유세균 및 부유진균 조사 결과 ... 48
• 나. 계절별 총부유세균 및 부유진균의 농도 변화 ... 73
• 다. 계절별 부유진균의 농도 변화 ... 82
• 4. 총부유세균 및 부유진균의 결과 고찰 ... 87
• 5. 부유진균 분석방법(안) ... 91
• IV. 결 론 ... 92
• V. 참고문헌 ... 95
• VI. 부 록 ... 101
• 1. 부유진균의 시험방법(안) ... 101