초록 ▼

Ⅲ. 연구개발의 내용 및 범위
1. 연구개발의 내용 및 범위
1년차(2010):
목 표:- 바둑돌부전나비의 분포(남부),생태특성 및 생태관 도입 기술 개발
- 남방노랑나비 서식지 환경특성조사 및 실내 사육 기술 개발
- 대벌레 생태학적 특성조사 및 대체먹이 개발
- 땅강아지 생물학적 특징조사 및 인공사료를 이용한 사육시스템 개발
내 용: - 분포조사
- 소장표본조사
- 생태특성조사
- 서식지 조사
-

Ⅲ. 연구개발의 내용 및 범위
1. 연구개발의 내용 및 범위
1년차(2010):
목 표:- 바둑돌부전나비의 분포(남부),생태특성 및 생태관 도입 기술 개발
- 남방노랑나비 서식지 환경특성조사 및 실내 사육 기술 개발
- 대벌레 생태학적 특성조사 및 대체먹이 개발
- 땅강아지 생물학적 특징조사 및 인공사료를 이용한 사육시스템 개발
내 용: - 분포조사
- 소장표본조사
- 생태특성조사
- 서식지 조사
- 생태특성 구명
- 분포 및 생태특성
- 먹이특성
- 생물학적특징조사
- 실내사육조건구명
- 인공사료개발
범 위: - 분포 : 제주 및 남부지역
- 소장표본 : 국내 대학, 연구소 등
- 생태조사 : 먹이식물, 한 살이 등
- 남방노랑나비 서식지 2개소 선정조사 및 생태 검증
- 남방노랑나비의 대량사육을 위한 사육조건 검증
- 분포 : 연천, 포천 등 경기지역
- 생태 : 서식처 환경, 단위생식 등
- 먹이특성 : 기주식물, 대체먹이
- 산란수, 발육기간, 수명
- 실내 누대 사육
- 인공사료제작
2년차(2011):
목 표: - 바둑돌부전나비의 분포(중부), 생태특성 및 생태관 도입 기술 개발
- 남방노랑나비 실내 사육을 위한 대체먹이 등 사육실험
- 대벌레 대체먹이를 이용한 사육시스템 개발
- 땅강아지 인공사육을 통한 학습용키트 개발
내 용: - 분포조사
- 소장표본조사
- 생태특성조사
- 대량사육 검증
- 대체먹이 검증
- 부화조건
- 산란특성
- 대체먹이
- 인공사육시스템개발
- 학습용 키트개발
범 위: - - 분포 : 충청 등 중부지역
- 소장표본 : 국내 대학, 연구소 등
- 생태조사 : 먹이식물, 한 살이,일본납작진딧물 생태 특성 등
- 남방노랑나비 대량사육법 개발
- 남방노랑나비 대체먹이 개발
- 부화조건 : 온도, 습도
- 산란특성 : 산란수, 산란기간
- 대체먹이 : 토끼풀
- 사육케이지, 먹이, 사육방식
- 사육키트(애완·체험학습용)
3년차(2012):
목 표: - 바둑돌부전나비의 분포(북부),생태특성 및 학습프로그램 개발
- 남방노랑나비 실내 사육 시스템확립 및 이용 기술개발
- 대벌레 대량사육시스템 확립 및 상품화 기술 개발
- 땅강아지 대량증식 시스템의 표준화 기술 개발 및 상품화
내 용: - 분포조사
- 소장표본조사
- 생태특성조사
- 동영상 및 프로그램 제작
- 사육체계 정립
- 사육메뉴얼제작
- 산업화
- 대량사육
- 상품화 기술
-대량사육시스템개발
- 상품화 기술개발
범 위: - 분포 : 경기 등 북부지역
- 소장표본 : 국내 대학, 연구소 등
- 생태조사 : 먹이식물, 한 살이,일본납작진딧물 생태 특성 등
- 바둑돌부전나비 한 살이 동영상, 장수풍뎅이 점핑크레이 만들기
- 남방노랑나비의 대량사육 시스템완성 및 매뉴얼작성
- 남방노랑나비 대량사육기술 특허출원 및 농가 기술 이전
- 대량사육밀도, 사육체계
- 상품화 : 인공먹이, 사육키트
- 인공대량생산기술 매뉴얼작성
- 온라인판매

Abstract ▼

《제1세부과제》
Taraka hamada (Druce, 1875) is the sole predaceous butterfly species, which feeds only on Ceratovacuna japonica of Telaxidae, in Korea. This study was performed to examine the extends of distribution and the ecological characteristics of the species, which has not known from the prior st

《제1세부과제》
Taraka hamada (Druce, 1875) is the sole predaceous butterfly species, which feeds only on Ceratovacuna japonica of Telaxidae, in Korea. This study was performed to examine the extends of distribution and the ecological characteristics of the species, which has not known from the prior study. First, the limit of distribution of T. hamada restricted from Jeju Island to Han river of the middle Korea throughout the 25 examined specimens preserved at the collection roos of four related institutes and 89 local sites in filed surveys.
To examine genetic divergences within T. hamada and Ceratovacuna japonica (Takahashi), we analysed DNA barcoding region of COI gene in mitochondria. the sequences of 658 bp in length were identical with the compared sequences in NCBI. The host plant of C. japonica was identified as Pseidosasa japonica (Sieb. et Zucc.) Makino. C. japonica widely distributed at a host plant, but more densely congregated at the basal of a leaf.
The larvae of T. hamada also distributed at the basal of a leaf and undergo to 4th larval stages. The adults of this species have a week spot that killed within 112 seconds, when they were exposed to direct light. To introduce the butterfly into inner insectarium, a glasshouse was examined as the most suitable rearing system. To breed C. japonica, each individual removed using a pin to others of the host plant that was recognized as the most effectiveness in this study.
Finally, we produced a video on the life cycle of T. hamata and established the making method for Allomyrina dichotoma to contribute an educational program based on experience study
《제2세부과제》
This studies performed for development of artificial rearing system of common grass yellow butterfly, Eurema hecabe. For successful study, we investigated ecological environments and indoor-rearing conditions of Eurema hecabe for artificial rearing. Two different nesting site, a open area nearby small river(A area; Daejin-ri, Gonyang-myeon, Sachun-si, Gyoungsangnam-do) and a foot of a hill area(B area;Noryang-ri, Seolcheon-myeon, Namhae-gun, Kyoungsangnam-do), selected for investigation of ecological habitat environment, and investigated two times per month,during six month, from June to November in 2010. In result, the number of butterflies, Eurema hecabe, at the hill area(B area) was 6 times more than the open area(A area). We observed the number of eggs, larvae and puapae in the B area more times compared to those in the A area. In the B area, Lespedeza cuneata was higher preference than the other host plant, Lespedeza pilosa and Albizia julibrissin.
For development of indoor-rearing conditions, common grass yellow butterfly, Eurema hecabe, was reared in a room condition. As a result of oviposition preference experiment, 100-mated-female laid significantly higher number of eggs 104.9±19.6 on Lespedeza cuneata plant, whereas the number of eggs was 12.7±4.5 on Cassia obtusifolia plant. Moreover, the eggs hatched within 5.1±0.9 days from the day of oviposition and procent of egg hatchability was 53.7% on host plant. The headwidth of each developmental larval stage were 0.36±0.02(1st), 0.61±0.02(2nd), 0.93±0.05(3rd), 1.46±0.08(4th), 2.25±0.11(5th). The larval period was 12.1±0.9 days under high temperature, long day condition(25℃, 16L:8D), showing 81.0% pupal ratio. The pupal period was 6.9±0.7 days, and the emergence rate was 79.6%. 5-artificial diet were developed for untimely rearing of butterfly. In result of diet-test, C-diet, 20% contained host plant leaf(Lespedeza cuneata), was higher survival rate than the others. There are 82% pupal ratio, 77% emergence rate on C-diet. Based on above experiment, artificial rearing system of common grass yellow butterfly, Eurema hecabe was completed in indoor condition. For effective distribution of this system, we applicated for two patent, and carried out two technology transfer.
《제3세부과제》
Baculum elongatum has several peculiarities like parthenogenesis, unique external features, changing body color and dropping oviposition, which makes it a very hopeful to use industrial insect.
The oviposition was established by only female without fertilization with sperm of male. The oviposition period was 42.2±22.7 days and the number of eggs per female was 109.5±70.5 eggs. The hatchability was 73.3% at 25℃ and 66.7% after low temperature treatment(8℃, 60 days). In nature, B. elongatum overwinter as egg, but it can be assumed from this result that cold temperature was not needed for hatching. The hatchability was 98.2% in the treatment of floral foam+fermented sawdust+eggs+leaves. The developmental period was 100.9±4.2 days at eggs, 55.3±4.6 days at nymph and 49.7±16.0 days at adult. The length of egg was 0.33±0.0cm and the lengths of the nymph were clearly classified according to instar stage. Clover(Trifolium repens) was excellent diet as like host plant and can be used alternative diet. The adult rate of insect was 66.7% in the artificial diet containing 25% of acacia leaves. For sustaining mass rearing the nymph or adult of B. elongatum, it can be used that natural diet as like acacia(Robinia seudoacacia), white oak(Quercus aliena), chestnut(Castanea crenata var dulcis) and bush clover(Lespedeza bicolor) or alternative diet as like clover or artificial diet.
《제4세부과제》
The studies were conducted to investigate on the biological characteristics of mole cricket, Gryllotalpa orientalis (Orthoptera: Gryllotalpidae) and laboratory evaluation of artificial diet for G. orientalis. The mean values of preoviposition duration and the longevity of overwintering individuals were 35.5 and 85.0 days, respectively. The mean value of fecundity 55.2 eggs and the mean number of eggs per egg cells were 35.7 eggs. The mean of egg size was 2.5 x 1.2 mm and developmental period of egg stage was 18.5 days. The nympal development period from 1st to 8th was 12.9±1.3, 9.3±0.6, 8.5±2.9, 15.8±1.9, 19.3±5.7, 16.8±2.2, 21.4±2.7 and 23.6±4.8 days, respectively. A whole mean developmental period of 116.9±11.5 days. One hundred days after the hatching of G. orientalis nymphs was investigated the body length and developmental stages at the three different temperature ranged; at 21℃, 6th nympal instar 1.7±0.1㎝, at 25℃, 6th and 7th nympal instar 1.9±0.1㎝, at 27℃, 7th and 8th nympal instar 2.4±0.4㎝. The compos tion of the nutrients in artificial diet used for G. orientalis in our study were made with wheat germ (66.1%), casein (18.4%), salt mix. (5.9%), sorbic acid (1.5%), methyl paraben (0.7%) and vitamin mix (7.3%). The survival rate of G. orientalis for a total 180 individuals reared on the artificial diet from egg to adult were 89.1%. The present study is to develop the rearing method of Gryllotalpa orientalis. In total 429 of G. orientalis were collected from the field rearing cage (25㎡) in 2012. Its sex ratio was 1: 1.15(Female : Male). Survival rate of the mole crickets was 94.4∼86.1% with the artificial diets formulated for the present study. Successful oviposition rate was 20, 20 and 80% for one, two and three pairs of adult crickets, respectively, from the indoor rearing. The mean number of hatchlings was 11.8±21.7, 15.7±26.4 and 25.8±38.8, and the mean number of dead hatchlings 1.2, 1.7 and 1.2. The mortality of nymphs on horticultural soil and clay sand mixed with ocher was 18.3 and 10.0%, respectively. The mortality of nymphs in circular and rectangular cages was, respectively, 60 and 40%.

목차 Contents

• 표지 ... 1
• 제출문 ... 2
• 요약문 ... 3
• SUMMARY ... 9
• 목차 ... 12
• 제 1 장 서 론 ... 13
• 제 1 절 : 육식성 바둑돌부전나비의 분포 및 생태학적 특성 조사 ... 13
• 제 2 절 : 남방노랑나비의 실내 사육시스템 확립 및 산업화 이용에 관한 연구 ... 14
• 제 3 절 : 대벌레류의 실내 사육시스템 확립 및 산업화 이용 연구 ... 15
• 제 4 절 : 땅강아지의 실내 사육시스템 확립 및 산업화 이용 연구 ... 15
• 제 2 장 국내외 기술개발 현황 ... 17
• 제 1 절 : 육식성 바둑돌부전나비의 분포 및 생태학적 특성 조사 ... 17
• 제 2 절 : 남방노랑나비의 실내 사육시스템 확립 및 산업화 이용에 관한 연구 ... 18
• 제 3 절 : 대벌레류의 실내 사육시스템 확립 및 산업화 이용 연구 ... 20
• 제 4 절 : 땅강아지의 실내 사육시스템 확립 및 산업화 이용 연구 ... 20
• 제 3 장 연구개발수행 내용 및 결과 ... 21
• 제1절 육식성 바둑돌부전나비의 분포 및 생태학적 특성 조사 ... 21
• 제3절 대벌레류의 실내 사육시스템 확립 및 산업화 이용 연구 ... 65
• 제4절 땅강아지의 실내 사육시스템 확립 및 산업화 이용 연구 ... 72
• 제 4 장 연구개발목표 달성도 및 대외기여도 ... 96
• 1절 목표대비 대외 달성도 ... 96
• 2절 정량적 성과 ... 98
• 제 5 장 연구개발결과의 활용계획 ... 102
• 제 6 장 연구개발과정에서 수집한 해외과학기술정보 ... 104
• 제 7 장 기타 중요 변동사항 ... 105
• 제 8 장 국가과학기술종합정보시스템에 등록한 연구장비현황 ... 106
• 제 9 장 참고문헌 ... 107